Q:

Is it possible to quantify the mycoplasma with the MycoAlert® Mycoplasma Detection Assay?

A:

No, the MycoAlert® Mycoplasma Detection Assay (as well as the MycoAlert® PLUS Mycoplasma Detection Assay) is not quantitative;

it has been designed to give a simple Yes or No answer, pertaining to the presence of Mycoplasma.

Categories:
Mycoplasma Detection and Prevention
Research Areas:
Cancer Research/Cell Biology
Basic Research
Molecular Biology

You may also want to know:

What are mycoplasmas?
Do the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits detect dead mycoplasma?
Can mycoplasma grow outside of a cell?
Can mycoplasma grow in the absence of cells?
How can cells be cured of mycoplasma contamination?
How can cells be cured of mycoplasma contamination?
Does the MycoAlert® Assay recognize the whole spectrum of mycoplasma?
I tested my cell culture supernatant negative for mycoplasma contamination using the MycoAlert® Mycoplasma Detection Kit or the MycoAlert® PLUS Mycoplasma Detection Kit. But when I tested the same cells using a PCR-based Assay, I obtained a positive result.
How can I detect mycoplasma contamination of my cells?
For Lonza’s MycoAlert® Mycoplasma Detection Assays is it possible to convert the B/A value of positive control included in the MycoAlert® Assay Control Set to CFU of mycoplasma?
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