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Is it possible to quantify the
mycoplasma
with the MycoAlert®
Mycoplasma
Detection Assay?
No, the MycoAlert®
Mycoplasma
Detection Assay (as well as the MycoAlert® PLUS
Mycoplasma
Detection Assay) is not quantitative; it has been designed to give a simple Yes or No answer, pertaining to the presence of
Mycoplasma
.
What are
mycoplasma
s?
Mycoplasmas
belong to the family Mollicutes, which includes Acholeplasma, Ureaplasma and other species.However the term "
Mycoplasma
" is most often used as a "cover-all".More than 180 species have been identified of which 20 distinct
Mycoplasma
...
Do the MycoAlert® and MycoAlert® PLUS
Mycoplasma
Detection Kits detect dead
mycoplasma
?
No. Lonza's MycoAlert® and MycoAlert® PLUS Kits only detect live
mycoplasma
which gives an accurate assesment of contamination. PCR based detection methods dectect both living and dead
mycoplasma
, which can lead to false positives.
Can
mycoplasma
grow outside of a cell?
This is an area that is up for debate. There is evidence that
mycoplasma
can survive in the absence of cells, and some may proliferate, but as a rule they are much happier if cells are present.
Can
mycoplasma
grow in the absence of cells?
There is evidence to suggest that
mycoplasma
can survive in the absence of cells, some may even proliferate, but as a rule they are much more viable if cells are present.
How can cells be cured of
mycoplasma
contamination?
Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™
Mycoplasma
Elimination Reagent has been optimized to eliminate
mycoplasma
with minimal toxic effects on the cells. The MycoZap™ Reagent eliminates...
How can cells be cured of
mycoplasma
contamination?
Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™
Mycoplasma
Elimination Reagent has been optimized to eliminate
mycoplasma
with minimal toxic effects on the cells.The MycoZap™ Reagent eliminates...
Does the MycoAlert® Assay recognize the whole spectrum of
mycoplasma
?
The
mycoplasma
specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (
Mycoplasma
, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in cell culture). 95...
I tested my cell culture supernatant negative for
mycoplasma
contamination using the MycoAlert®
Mycoplasma
Detection Kit or the MycoAlert® PLUS
Mycoplasma
Detection Kit. But when I tested the same cells using a PCR-based Assay, I obtained a positive result.
It is possible that the PCR is picking up dead
mycoplasma
whereas the MycoAlert® and MycoAlert® Plus Assay will pick up only viable
mycoplasma
. It is possible of course that there is contamination in the PCR giving a false positive.
How can I detect
mycoplasma
contamination of my cells?
We recommend using the 20-minute MycoAlert®
Mycoplasma
Detection Kit from Lonza. The simple four-step MycoAlert® Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid...
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