Q:

Do the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits detect dead mycoplasma?

A:

No. Lonza's MycoAlert® and MycoAlert® PLUS Kits only detect live mycoplasma which gives an accurate assesment of contamination. PCR based detection methods dectect both living and dead mycoplasma, which can lead to false positives. 

Categories:
Mycoplasma Detection and Prevention
Bioassay
Research Areas:
Basic Research

You may also want to know:

Is it possible to quantify the mycoplasma with the MycoAlert® Mycoplasma Detection Assay?
What are mycoplasmas?
Can mycoplasma grow outside of a cell?
Can mycoplasma grow in the absence of cells?
How can cells be cured of mycoplasma contamination?
How can cells be cured of mycoplasma contamination?
Does the MycoAlert® Assay recognize the whole spectrum of mycoplasma?
I tested my cell culture supernatant negative for mycoplasma contamination using the MycoAlert® Mycoplasma Detection Kit or the MycoAlert® PLUS Mycoplasma Detection Kit. But when I tested the same cells using a PCR-based Assay, I obtained a positive result.
How can I detect mycoplasma contamination of my cells?
For Lonza’s MycoAlert® Mycoplasma Detection Assays is it possible to convert the B/A value of positive control included in the MycoAlert® Assay Control Set to CFU of mycoplasma?
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