SMC, coronary artery, human (CASMC)

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Primary smooth muscle cells isolated from human coronary artery

Cell Type:
Smooth muscle cell
Tissue Origin:
artery
Species:
human
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Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

Smooth Muscle Growth Medium-2

 SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Smooth muscle, basic A-033 2e6 90% 84% Plasmid (general) 2 µg 100 µl I/II/2b
P1

FP-113

 1e5 67% 86% Plasmid (general) 0.2 µg 20 µl 4D X-Unit
P1

FP-113

 1e5 67% 86% Plasmid (general) 0.2 µg 20 µl 96-well

Citations (27)

Dual biofunctional polymer modifications to address endothelialization and smooth muscle cell integration of ePTFE vascular grafts.
Categories:
Primary Cells and Media, 3D Cell Culture , Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bastijanic JM, Kligman FL, Marchant RE, Kottke-Marchant K 
In:
J Biomed Mater Res A (2016) 104(1): 71-81 
A selective microRNA-based strategy inhibits restenosis while preserving endothelial function
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Santulli G, Wronska A, Uryu K, Diacovo TG, Gao M, Marx SO, Kitajewski J, Chilton JM, Akat KM, Tuschl T, Marks AR, Totary-Jain H 
In:
J Clin Invest (2014) 124(9): 4102-14 
PPARa: A Master Regulator of Bilirubin Homeostasis.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bigo C1, Kaeding J1, El Husseini D1, Rudkowska I2, Verreault M1, Vohl MC3, Barbier O1 
In:
Other (2014) 00: 747014 
Disease-related growth factor and embryonic signaling pathways modulate an enhancer of TCF21 expression at the 6q23.2 coronary heart disease locus
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Miller CL, Anderson DR, Kundu RK, Raiesdana A, Nürnberg ST, Diaz R, Cheng K, Leeper NJ, Chen CH, Chang IS, Schadt EE, Hsiung CA, Assimes TL, Quertermous T 
In:
PLoS ONE (2013) 9(7): e1003652 
Identification of human embryonic progenitor cell targeting peptides using phage display
Categories:
Primary Cells and Media 
Authors:
Bignone PA, Krupa RA, Sternberg H, Funk WD, Snyder EY, West MD, Larocca D 
In:
PLoS ONE (2013) 8(3): e58200 
Bone morphogenetic protein-2 decreases microRNA-30b and microRNA-30c to promote vascular smooth muscle cell calcification
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Balderman JA, Lee HY, Mahoney CE, Handy DE, White K, Annis S, Lebeche D, Hajjar RJ, Loscalzo J, Leopold JA 
In:
J Am Heart Assoc (2012) 1(6): E003905 
Post-transcriptional regulation of placenta growth factor mRNA by hydrogen peroxide
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Shaw JH, Lloyd PG 
In:
Microvasc Res (2012) 84(2): 155-60 
Differential susceptibility of human primary aortic and coronary artery vascular cells to RNA interference.
Categories:
Primary Cells and Media 
Authors:
Nabzdyk CS, Chun M, Pradhan Nabzdyk L, Yoshida S, LoGerfo FW 
In:
Biochim Biophys Acta (2012) 425(2): 261-5 
Gene expression profile of coronary artery cells treated with nonsteroidal anti-inflammatory drugs reveals off-target effects
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Palayoor ST, J-Aryankalayil M, Makinde AY, Cerna D, Falduto MT, Magnuson SR, Coleman CN. 
In:
J Cardiovasc Pharmacol (2012) 59(6): 487-99 
Reciprocal regulation of syndecan-2 and Notch signaling in vascular smooth muscle cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhao N, Liu H, Lilly B 
In:
J Biol Chem (2012) 287(20): 16111-20 
Bindarit inhibits human coronary artery smooth muscle cell proliferation, migration and phenotypic switching
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Maddaluno M, Grassia G, Di Lauro MV, Parisi A, Maione F, Cicala C, De Filippis D, Iuvone T, Guglielmotti A, Maffia P, Mascolo N, Ialenti A 
In:
PLoS ONE (2011) 7(10): e47464 
Three-dimensional topography of synthetic scaffolds induces elastin synthesis by human coronary artery smooth muscle cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lin S, Sandig M, Mequanint K 
In:
Tissue Eng (2011) 17(11-12): 1561-71 
Smooth muscle homeostasis in human atherosclerotic plaques through interleukin 15 signalling
Categories:
Primary Cells and Media 
Authors:
van der Meer JJ, de Boer OJ, Teeling P, van der Loos CM, Dessing MC, van der Wal AC 
In:
Int J Clin Exp Pathol (2011) 4(3): 287-94 
Reciprocal regulation of 11ß-hydroxysteroid dehydrogenase 1 and glucocorticoid receptor expression by dexamethasone inhibits human coronary artery smooth muscle cell proliferation in vitro.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Michas G, Liberman M, Becker KC, Handy DE, Loscalzo J, Leopold JA 
In:
Mol Cell Biol (2011) 346(1-2): 69-79 
Use of human mesenchymal stem cells as alternative source of smooth muscle cells in vessel engineering
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gong Z, Niklason LE 
In:
Methods Mol Biol (2011) 698: 279-94 
Transforming growth factor-beta1 regulation of C-type natriuretic peptide expression in human vascular smooth muscle cells: dependence on TSC22D1
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mendonça MC, Koles N, Doi SQ, Sellitti DF. 
In:
Am J Physiol Heart Circ Physiol (2010) 299(6): H2018-27 
Chromatin remodeling pathways in smooth muscle cell differentiation, and evidence for an integral role for p300
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Spin JM, Quertermous T, Tsao PS 
In:
PLoS ONE (2010) 5(12): e14301 
Adipose tissue progenitor cells directly interact with endothelial cells to induce vascular network formation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Merfeld-Clauss S, Gollahalli N, March KL, Traktuev DO. 
In:
Tissue Eng (2010) 16(9): 2953-66 
Goniothalamin Induces Coronary Artery Smooth Muscle Cells Apoptosis: The p53-Dependent Caspase-2 Activation Pathway
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chan KM, Rajab NF, Siegel D, Din LB, Ross D, Inayat-Hussain SH 
In:
Toxicol Sci (2010) 116(2): 533-48 
Fractalkine has anti-apoptotic and proliferative effects on human vascular smooth muscle cells via epidermal growth factor receptor signalling
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
White GE, Tan TC, John AE, Whatling C, McPheat WL, Greaves DR 
In:
Cardiovasc Res (2010) 85(4): 825-35 
The d5 dopamine receptor mediates large-conductance, calcium- and voltage-activated potassium channel activation in human coronary artery smooth muscle cells
Categories:
Primary Cells and Media 
Authors:
Natarajan A, Han G, Chen SY, Yu P, White R, Jose P 
In:
J Pharmacol Exp Ther (2010) 332(2): 640-9 
Atherogenic lipids induce high-density lipoprotein uptake and cholesterol efflux in human macrophages by up-regulating transmembrane chemokine CXCL16 without engaging CXCL16-dependent cell adhesion
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Barlic J, Zhu W, Murphy PM 
In:
J Immunol (2009) 182(12): 7928-36 
Pleiotrophin induces nitric oxide dependent migration of endothelial progenitor cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Heiss C, Wong ML, Block VI, Lao D, Real WM, Yeghiazarians Y, Lee RJ, Springer ML 
In:
J Cell Physiol (2008) 215(2): 366-73 
Atherogenic lipids induce adhesion of human coronary artery smooth muscle cells to macrophages by upregulating chemokine CX3CL1 on smooth muscle cells in a TNFalpha -NFkappa B-dependent manner
Categories:
Transfection 
Authors:
Barlic J, Zhang Y, Murphy PM 
In:
J Biol Chem (2007) 282(26): 19167-76 
Urokinase-induced signaling in human vascular smooth muscle cells is mediated by PDGFR-beta
Categories:
Transfection 
Authors:
Kiyan J, Kiyan R, Haller H and Dumler I 
In:
EMBO J (2005) 24(10): 1787-1797 
Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells
Categories:
Transfection 
Authors:
Iversen N, Birkenes B, Torsdalen K and Djurovic S 
In:
Genet Vaccines Ther (2005) 3(1): 2 
Upregulation of the cytoskeletal-associated protein Moesin in the neointima of coronary arteries after balloon angioplasty: a new marker of smooth muscle cell migration?
Categories:
Transfection 
Authors:
Blindt R, Zeiffer U, Krott N, Filzmaier K, Voss M, Hanrath P, vom Dahl J, Bosserhoff AK 
In:
Cardiovasc Res (2002) 54(3): 630-9 
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