SMC, coronary artery, human (CASMC)

Primary smooth muscle cells isolated from human coronary artery

Cell Type:
Smooth muscle cell
Tissue Origin:
artery
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

 SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Smooth muscle, basic A-033 2e6 90% 84% Plasmid (general) 2 µg 100 µl I/II/2b
P1

FP-113

1e5 67% 86% Plasmid (general) 0.2 µg 20 µl 4D X-Unit
P1

FP-113

1e5 67% 86% Plasmid (general) 0.2 µg 20 µl Shuttle

Citations (27)

Categories:
Primary Cells and Media, 3D Cell Culture , Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bastijanic JM, Kligman FL, Marchant RE, Kottke-Marchant K 
In:
J Biomed Mater Res A (2016) 104(1): 71-81 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Santulli G, Wronska A, Uryu K, Diacovo TG, Gao M, Marx SO, Kitajewski J, Chilton JM, Akat KM, Tuschl T, Marks AR, Totary-Jain H 
In:
J Clin Invest (2014) 124(9): 4102-14 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bigo C1, Kaeding J1, El Husseini D1, Rudkowska I2, Verreault M1, Vohl MC3, Barbier O1 
In:
Other (2014) 00: 747014 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Miller CL, Anderson DR, Kundu RK, Raiesdana A, Nürnberg ST, Diaz R, Cheng K, Leeper NJ, Chen CH, Chang IS, Schadt EE, Hsiung CA, Assimes TL, Quertermous T 
In:
PLoS ONE (2013) 9(7): e1003652 
Categories:
Primary Cells and Media 
Authors:
Bignone PA, Krupa RA, Sternberg H, Funk WD, Snyder EY, West MD, Larocca D 
In:
PLoS ONE (2013) 8(3): e58200 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Balderman JA, Lee HY, Mahoney CE, Handy DE, White K, Annis S, Lebeche D, Hajjar RJ, Loscalzo J, Leopold JA 
In:
J Am Heart Assoc (2012) 1(6): E003905 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Shaw JH, Lloyd PG 
In:
Microvasc Res (2012) 84(2): 155-60 
Categories:
Primary Cells and Media 
Authors:
Nabzdyk CS, Chun M, Pradhan Nabzdyk L, Yoshida S, LoGerfo FW 
In:
Biochim Biophys Acta (2012) 425(2): 261-5 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Palayoor ST, J-Aryankalayil M, Makinde AY, Cerna D, Falduto MT, Magnuson SR, Coleman CN. 
In:
J Cardiovasc Pharmacol (2012) 59(6): 487-99 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhao N, Liu H, Lilly B 
In:
J Biol Chem (2012) 287(20): 16111-20 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Maddaluno M, Grassia G, Di Lauro MV, Parisi A, Maione F, Cicala C, De Filippis D, Iuvone T, Guglielmotti A, Maffia P, Mascolo N, Ialenti A 
In:
PLoS ONE (2011) 7(10): e47464 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lin S, Sandig M, Mequanint K 
In:
Tissue Eng (2011) 17(11-12): 1561-71 
Categories:
Primary Cells and Media 
Authors:
van der Meer JJ, de Boer OJ, Teeling P, van der Loos CM, Dessing MC, van der Wal AC 
In:
Int J Clin Exp Pathol (2011) 4(3): 287-94 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Michas G, Liberman M, Becker KC, Handy DE, Loscalzo J, Leopold JA 
In:
Mol Cell Biol (2011) 346(1-2): 69-79 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gong Z, Niklason LE 
In:
Methods Mol Biol (2011) 698: 279-94 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mendonça MC, Koles N, Doi SQ, Sellitti DF. 
In:
Am J Physiol Heart Circ Physiol (2010) 299(6): H2018-27 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Spin JM, Quertermous T, Tsao PS 
In:
PLoS ONE (2010) 5(12): e14301 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Merfeld-Clauss S, Gollahalli N, March KL, Traktuev DO. 
In:
Tissue Eng (2010) 16(9): 2953-66 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chan KM, Rajab NF, Siegel D, Din LB, Ross D, Inayat-Hussain SH 
In:
Toxicol Sci (2010) 116(2): 533-48 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
White GE, Tan TC, John AE, Whatling C, McPheat WL, Greaves DR 
In:
Cardiovasc Res (2010) 85(4): 825-35 
Categories:
Primary Cells and Media 
Authors:
Natarajan A, Han G, Chen SY, Yu P, White R, Jose P 
In:
J Pharmacol Exp Ther (2010) 332(2): 640-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Barlic J, Zhu W, Murphy PM 
In:
J Immunol (2009) 182(12): 7928-36 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Heiss C, Wong ML, Block VI, Lao D, Real WM, Yeghiazarians Y, Lee RJ, Springer ML 
In:
J Cell Physiol (2008) 215(2): 366-73 
Categories:
Transfection 
Authors:
Kiyan J, Kiyan R, Haller H and Dumler I 
In:
EMBO J (2005) 24(10): 1787-1797 
Categories:
Transfection 
Authors:
Iversen N, Birkenes B, Torsdalen K and Djurovic S 
In:
Genet Vaccines Ther (2005) 3(1): 2 
Categories:
Transfection 
Authors:
Blindt R, Zeiffer U, Krott N, Filzmaier K, Voss M, Hanrath P, vom Dahl J, Bosserhoff AK 
In:
Cardiovasc Res (2002) 54(3): 630-9 
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