Dual biofunctional polymer modifications to address endothelialization and smooth muscle cell integration of ePTFE vascular grafts.

Bastijanic JM, Kligman FL, Marchant RE, Kottke-Marchant K
Source: J Biomed Mater Res A
Publication Date: (2016)
Issue: 104(1): 71-81
Research Area:
Cells used in publication:
SMC, coronary artery, human (CASMC)
Species: human
Tissue Origin: artery
Endothelial, pulmonary artery (HPAEC), human
Species: human
Tissue Origin: artery
Lonza\\\'s HPAECs and CASMCs were used in an experiment with ePTFE grafts. Results were compared to fibronectin controls. The grafts in this experiment were shown to be able to be simultaneously modified with two different polymers. This could help with both endothelialization and intimal hyperplasia.
Expanded polytetrafluoroethylene (ePTFE) grafts were coated on the luminal surface with a cell-adhesive fluorosurfactant (FSP) polymer to promote endothelialization, followed by ethanol hydration to degas the pores and subsequent cell-adhesive, enzymatically degradable poly(ethylene glycol)-based hydrogel incorporation into the graft interstices to accommodate potential smooth muscle cell integration in the graft wall. The FSP coating on ePTFE was stable as demonstrated by a significantly reduced receding water contact angle on FSP-coated ePTFE (14.5?±?6.4°) compared to uncoated ePTFE (105.3?±?4.5°, P?