Reciprocal regulation of 11ß-hydroxysteroid dehydrogenase 1 and glucocorticoid receptor expression by dexamethasone inhibits human coronary artery smooth muscle cell proliferation in vitro.

Michas G, Liberman M, Becker KC, Handy DE, Loscalzo J, Leopold JA
Source: Mol Cell Biol
Publication Date: (2011)
Issue: 346(1-2): 69-79
Research Area:
Basic Research
Cells used in publication:
SMC, coronary artery, human (CASMC)
Species: human
Tissue Origin: artery
The actions of glucocorticoids are mediated, in part, by 11ß-hydroxysteroid dehydrogenase 1 (11ß-HSD1), which amplifies their effects at the pre-receptor level by converting cortisone to cortisol. Glucocorticoids, such as dexamethasone, inhibit vascular smooth muscle cell proliferation; however, the role of 11ß-HSD1 in this response remains unknown. Accordingly, we treated human coronary artery smooth muscle cells (HCSMC) with dexamethasone (10(-9)-10(-6) mol/l) and found that after 72 h dexamethasone increased 11ß-HSD1 expression (14.16 ± 1.6-fold, P < 0.001) and activity (6.21 ± 1.2-fold, P < 0.001) in a dose- and time-dependent manner, which was dependent upon glucocorticoid receptor (GR) activation and C/EBPß and C/EBPd signaling. As glucocorticoids are known to negatively regulate GR expression, we examined the effect of decreasing 11ß-HSD1 expression on GR expression. In HCSMC transfected with 11ß-HSD1 siRNA, GR expression was increased; this effect was associated with protein kinase A activation and CREB phosphorylation. To examine the role of 11ß-HSD1 in HCSMC proliferation, we decreased 11ß-HSD1 expression and stimulated cells with platelet-derived growth factor (PDGF) (10 ng/ml). Decreased 11ß-HSD1 expression was associated with increased cell proliferation in the absence of PDGF compared to scrambled control-transfected cells (236.10 ± 13.11%, n = 4, P < 0.001) and this effect was augmented by PDGF. Furthermore, the inhibitory effect of dexamethasone on cellular proliferation was abrogated in 11ß-HSD1 siRNA-transfected HCSMC. Downregulation of 11ß-HSD1 was associated with decreased p27(kip1) expression and increased phosphorylated retinoblastoma protein, consistent with a proliferative response. These findings suggest that 11ß-HSD1 plays a role in the effects of glucocorticoids on vascular smooth muscle cell phenotype.