T cell, mouse - C57BL/6

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T lymphocytes isolated from mouse spleen.

Cell Type:
T Cell
Tissue Origin:
blood
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3

DN-100

 2e6 35-45% 25-30% Plasmid (general) 0.5 µg 20 µl 4D X-Unit
T cell, mouse X-001 5e6 21-33% 42-48% Plasmid (general) 2.5 µg 100 µl I/II/2b
T cell, mouse X-001 5e6 21-35% 21-29% Plasmid (general) 2.5 µg 100 µl I/II/2b
T cell, mouse X-001 5e6 18-28% 35-47% Plasmid (general) 2.5 µg 100 µl I/II/2b
T cell, mouse X-001 5e6 15-25% 12-21% Plasmid (general) 2.5 µg 100 µl I/II/2b
P3 96-DN-100 2e6 34-52% 14-32% Plasmid (general) 0.5 µg 20 µl 96-well

Citations (33)

An evolved AAV variant enables efficient genetic engineering of murine T cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY, Xie WH, Allain V, Steinhart Z, Chang C, Talbot A, Kim S, Rosales A, Havlik LP, Pimentel H, Asokan A, Eyquem J 
In:
Cell (2023) 186(2): 446-460 
Naïve Primary Mouse CD8+ T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Pfenninger P, Yerly L, Abe J 
In:
Frontiers in Immunology (2022) 13: 777113 
Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Oh SA, Seki A, Rutz S.  
In:
Curr Protoc Immunol (2019) 124(1): e69 
Brain regulatory T cells suppress astrogliosis and potentiate neurological recovery.
Categories:
Primary Cells and Media, Transfection 
Authors:
Ito M, Komai K, Mise-Omata S, Iizuka-Koga M, Noguchi Y, Kondo T, Sakai R, Matsuo K, Nakayama T, Yoshie O, Nakatsukasa H, Chikuma S, Shichita T, Yoshimura A. 
In:
Nature (2019) 565 (7738): 246-250 
Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Seki A, Rutz S. 
In:
J Exp Med (2018) 215(3): 985-997 
Comparison of Lentiviral and Sleeping Beauty Mediated alpha beta T Cell Receptor Gene Transfer
Categories:
Transfection 
Authors:
Anne-Christine Field, Conrad Vink, Richard Gabriel, Roua Al-Subki, Manfred Schmidt, Nicholas Goulden, Hans Stauss, Adrian Thrasher, Emma Morris, Waseem Qasim 
In:
PLoS ONE (2013) 8(6): e68201 
Prostaglandin E2 promotes Th1 differentiation via synergistic amplification of IL-12 signalling by cAMP and PI3-kinase.
Categories:
Transfection 
Authors:
Yao C1, Hirata T, Soontrapa K, Ma X, Takemori H, Narumiya S. 
In:
Nat Commun. (2013) 4: 1685 
Cutting edge: suppression of GM-CSF expression in murine and human T cells by IL-27.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Young A1, Linehan E, Hams E, O'Hara Hall AC, McClurg A, Johnston JA, Hunter CA, Fallon PG, Fitzgerald DC 
In:
J Immunol (2012) 189 (5): 2079-83 
Protein kinase C delta localizes to secretory lysosomes in CD8+ CTL and directly mediates TCR signals leading to granule exocytosis-mediated cytotoxicity
Categories:
Transfection 
Authors:
Ma JS, Haydar TF, Radoja S 
In:
J Immunol (2008) 181(7): 4716-22 
NFATc1 regulates PD-1 expression upon T cell activation
Categories:
Transfection 
Authors:
Oestreich KJ, Yoon H, Ahmed R, Boss JM 
In:
J Immunol (2008) 181(7): 4832-9 
IFN regulatory factor-1 negatively regulates CD4+ CD25+ regulatory T cell differentiation by repressing Foxp3 expression
Categories:
Transfection 
Authors:
Fragale A, Gabriele L, Stellacci E, Borghi P, Perrotti E, Ilari R, Lanciotti A, Remoli AL, Venditti M, Belardelli F, Battistini A 
In:
J Immunol (2008) 181(3): 1673-82 
Primary peripheral T cells become susceptible to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD)-mediated apoptosis in vitro upon activation and in the presence of dendritic cells
Categories:
Transfection 
Authors:
Singh NP, Nagarkatti M, Nagarkatti P 
In:
Mol Pharmacol (2008) 73(6): 1722-35 
SNPs upstream of the minimal promoter control IL-2 expression and are candidates for the autoimmune disease-susceptibility locus Aod2/Idd3/Eae3
Categories:
Transfection 
Authors:
Del Rio R, Noubade R, Subramanian M, Saligrama N, Diehl S, Rincon M, Teuscher C 
In:
Genes Immun (2008) 9(2): 115-21 
Loss of Suppressor of Cytokine Signaling 1 in Helper T Cells Leads to Defective Th17 Differentiation by Enhancing Antagonistic Effects of IFN-gamma on STAT3 and Smads
Categories:
Transfection 
Authors:
Tanaka K, Ichiyama K, Hashimoto M, Yoshida H, Takimoto T, Takaesu G, Torisu T, Hanada T, Yasukawa H, Fukuyama S, Inoue H, Nakanishi Y, Kobayashi T, Yoshimura A 
In:
J Immunol (2008) 180(6): 3746-56 
A peptide that ameliorates lupus up-regulates the diminished expression of early growth response factors 2 and 3
Categories:
Transfection 
Authors:
Sela U, Dayan M, Hershkoviz R, Lider O, Mozes E 
In:
J Immunol (2008) 180(3): 1584-91 
Syndecan-4 Mediates the Coinhibitory Function of DC-HIL on T Cell Activation
Categories:
Transfection 
Authors:
Chung JS, Dougherty I, Cruz PD Jr, Ariizumi K 
In:
J Immunol (2007) 179(9): 5778-84 
A biochemical signature for rapid recall of memory CD4 T cells
Categories:
Transfection 
Authors:
Chandok MR, Okoye FI, Ndejembi MP, Farber DL 
In:
J Immunol (2007) 179(6): 3689-98 
Perforin activity and immune homeostasis: the common A91V polymorphism in perforin results in both pre- and post-synaptic defects in function
Categories:
Transfection 
Authors:
Voskoboinik I, Sutton VR, Ciccone A, House C, Chia J, Darcy PK, Yagita H, Trapani JA 
In:
Blood (2007) 110(4): 1184-90 
Comprehensive epigenetic profiling identifies multiple distal regulatory elements directing transcription of the gene encoding interferon-gamma
Categories:
Transfection 
Authors:
Schoenborn JR, Dorschner MO, Sekimata M, Santer DM, Shnyreva M, Fitzpatrick DR, Stamatoyonnapoulos JA, Wilson CB 
In:
Nat Immunol (2007) 8(7): 732-42 
Preferential expression of very late antigen-4 on type 1 CTL cells plays a critical role in trafficking into central nervous system tumors
Categories:
Transfection 
Authors:
Sasaki K, Zhu X, Vasquez C, Nishimura F, Dusak JE, Huang J, Fujita M, Wesa A, Potter DM, Walker PR, Storkus WJ, Okada H 
In:
Cancer Res (2007) 67(13): 6451-8 
Cyclic adenosine monophosphate is a key component of regulatory T cell-mediated suppression
Categories:
Transfection 
Authors:
Bopp T, Becker C, Klein M, Klein-Heßling S, Palmetshofer A, Serfling E, Heib V, Becker M, Kubach J, Schmitt S, Stoll S, Schild H, Staege MS, Stassen M, Jonuleit H, Schmitt E 
In:
J Exp Med (2007) 204(6): 1303-1310 
Protein Kinase C Regulates Antigen Receptor-Induced Lytic Granule Polarization in Mouse CD8+ CTL
Categories:
Transfection 
Authors:
Ma JS, Monu N, Shen DT, Mecklenbräuker I, Radoja N, Haydar TF, Leitges M, Frey AB, Vukmanovic S, Radoja S 
In:
J Immunol (2007) 178(12): 7814-21 
Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3
Categories:
Transfection 
Authors:
Hosokawa H, Kimura MY, Shinnakasu R, Suzuki A, Miki T, Koseki H, van Lohuizen M, Yamashita M, Nakayama T 
In:
J Immunol (2006) 177(11): 7656-64 
A critical role for TNF receptor-associated factor 1 and Bim down-regulation in CD8 memory T cell survival
Categories:
Transfection 
Authors:
Sabbagh L, Srokowski CC, Pulle G, Snell LM, Sedgmen BJ, Liu Y, Tsitsikov EN, Watts TH 
In:
Proc Natl Acad Sci USA (2006) 103(49): 18703-8 
Transcription factors TFE3 and TFEB are critical for CD40 ligand expression and thymus-dependent humoral immunity
Categories:
Transfection 
Authors:
Huan C, Kelly ML, Steele R, Shapira I, Gottesman SR, Roman CA 
In:
Nat Immunol (2006) 7(10): 1082-1091 
Enrichment of regulatory CD4(+)CD25(+) T cells by inhibition of phospholipase D signaling
Categories:
Transfection 
Authors:
Singh N, Seki Y, Takami M, Baban B, Chandler PR, Khosravi D, Zheng X, Takezaki M, Lee JR, Mellor AL, Bollag WB, Iwashima M 
In:
Nat Methods (2006) 3(8): 629-36 
Absence of the steroid receptor coactivator-3 induces B-cell lymphoma
Categories:
Transfection 
Authors:
Coste A, Antal MC, Chan S, Kastner P, Mark M, O'malley BW, Auwerx J 
In:
EMBO J (2006) 25(11): 2453-64 
Cutting Edge: Itk-Dependent Signals Required for CD4+ T Cells to Exert, but Not Gain, Th2 Effector Function
Categories:
Transfection 
Authors:
Au-Yeung BB, Katzman SD, Fowell DJ 
In:
J Immunol (2006) 176(7): 3895-9 
Coordination of NF-B and NFAT Antagonism by the Forkhead Transcription Factor Foxd1
Categories:
Transfection 
Authors:
Lin L, Peng SL 
In:
J Immunol (2006) 176(8): 4793-803 
Essential role of the T cell-specific adapter protein in the activation of LCK in peripheral T cells
Categories:
Transfection 
Authors:
Marti F, Garcia GG, Lapinski PE, Macgregor JN and King PD 
In:
J Exp Med (2006) 203(2): 281-7 
Recruitment and activation of PLCgamma1 in T cells: a new insight into old domains
Categories:
Transfection 
Authors:
Braiman A, Barda-Saad M, Sommers CL, Samelson LE 
In:
EMBO J (2006) 25(4): 774-84 
Transcriptional repressor DREAM regulates T-lymphocyte proliferation and cytokine gene expression
Categories:
Transfection 
Authors:
Savignac M, Pintado B, Gutierrez-Adan A, Palczewska M, Mellstrom B and Naranjo JR 
In:
EMBO J (2005) 24(20): 3555-3564 
c-Rel-Dependent Priming of Naive T Cells by Inflammatory Cytokines
Categories:
Transfection 
Authors:
Banerjee D, Liou HC and Sen R 
In:
Immunity (2005) 23(4): 445-458 
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