Epithelial, prostate (PrEC), human

Primary epithelial cells isolated from human prostate.

Prostate cells provide a glandular function in the body by generating fluid which serves several functions in
reproduction.

Cell Type:
Prostate Epith.
Tissue Origin:
prostate
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Prostate Epithelial Cell Growth Medium (PrEGM) BulletKit has been specially formulated for the serum-free maintenance and expansion of human prostate epithelial cells.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P1

CM-102

1e5

65-70%

45-50%

Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P1 96-CM-102 1e5 65-67% 40-50% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
Epithelial, basic

W-001

5e5 43-48% 65-70% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (83)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pan J1, Chen Y1, Mo C1, Wang D1, Chen J1, Mao X1, Guo S2, Zhuang J1, Qiu S1. 
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PLoS ONE (2014) 9(3): e92815 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Huang EC, Zhao Y, Chen G, Baek SJ, McEntee MF, Minkin S, Biggerstaff JP, Whelan J1. 
In:
BMC Complemen Altern Med (2014) 14:68: 1472 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Wong CP1, Hsu A1, Buchanan A2, Palomera-Sanchez Z1, Beaver LM1, Houseman EA2, Williams DE3, Dashwood RH3, Ho E4 
In:
PLoS ONE (2014) 9(1): e86787 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Bijnsdorp IV1, Geldof AA1, Lavaei M2, Piersma SR2, van Moorselaar RJ1, Jimenez CR 
In:
Other (2013) 23(2): 22097 
Categories:
Primary Cells and Media 
Authors:
Lee YM1, Lee SH1, Lee KB2, Nguyen MP1, Lee MY3, Park GH3, Kwon MJ 
In:
PLoS ONE (2013) 8(12): e84520 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yu Zeng1,3, Dong Gao1, John J Kim1,5, Takumi Shiraishi1, Naoki Terada1, Yoshiyuki Kakehi4, Chuize Kong3, Robert H Getzenberg1, Prakash Kulkarni1,2 
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Other (2013) 1(1): 39-52 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gao S1, Hsieh CL, Zhou J, Shemshedini L 
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PLoS ONE (2013) 8(11): e78766 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Paschke L1, Rucinski M, Ziolkowska A, Zemleduch T, Malendowicz W, Kwias Z, Malendowicz LK 
In:
Other (2013) 20: 453-59 
Categories:
Primary Cells and Media 
Authors:
Yu HJ1, Serebryannyy LA, Fry M, Greene M, Chernaya O, Hu WY, Chew TL, Mahmud N, Kadkol SS, Glover S, Prins G, Strakova Z, de Lanerolle P 
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PLoS ONE (2013) 8(11): e79776 
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PLoS ONE (2013) 8(8): 70515 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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PLoS ONE (2013) 8(8): 71637 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Biosci Rep (2013) 33(3): 00042 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Mol Cell (2013) 49(1): 80-93 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Reiner T, Parrondo R, de Las Pozas A, Palenzuela D, Perez-Stable C. 
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PLoS ONE (2013) 8(2): 56234 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sarveswaran S1, Gautam SC, Ghosh J. 
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Int J Oncol. (2012) 41(6): 2191-9 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Sakao K, Desineni S, Hahm ER, Singh SV. 
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Prostate (2012) 72(10): 1104-16 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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PLoS ONE (2012) 7(6): 40195 
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Primary Cells and Media 
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Prostate (2012) 72(6): 597-611 
Categories:
Primary Cells and Media 
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BMC Cancer (2012) 12: 145 
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J Med Chem (2012) 3827-36: 3827-36 
Categories:
Primary Cells and Media 
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Carcinogenesis (2012) 33(2): 413-9 
Categories:
Primary Cells and Media 
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Other (2012) 315(1): 48-58 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Free Radic Biol Med (2012) 52(2): 452-61 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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Cell Death Differ (2012) 3: 258 
Categories:
Primary Cells and Media 
Authors:
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Br J Cancer (2012) 106(1): 99-106 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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PLoS ONE (2012) 7(2): 32572 
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Primary Cells and Media 
Authors:
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PLoS ONE (2012) 7(10): 46617 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Mol Cancer Res (2012) 10(1): 34-9 
Categories:
Primary Cells and Media 
Authors:
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PLoS ONE (2012) 7(6): 39801 
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Primary Cells and Media 
Authors:
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OncoTarget (2011) 2(12): 1176-90 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gong Y, Li Y, Lu Y, Li L, Abdolmaleky H, Blackburn GL, Zhou JR. 
In:
Int J Cancer (2011) 129(5): 1042-52 
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Primary Cells and Media 
Authors:
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In:
J Med Chem (2011) 54(18): 6364-74 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cao Q, Mani RS, Ateeq B, Dhanasekaran SM, Asangani IA, Prensner JR, Kim JH, Brenner JC, Jing X, Cao X, Wang R, Li Y, Dahiya A, Wang L, Pandhi M, Lonigro RJ, Wu YM, Tomlins SA, Palanisamy N, Qin Z, Yu J, Maher CA, Varambally S, Chinnaiyan AM. 
In:
Cancer Cell (2011) 20(2): 187-99 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ross AE, Emadi A, Marchionni L, Hurley PJ, Simons BW, Schaeffer EM, Vuica-Ross M. 
In:
BJU Int. (2011) 108(3): 447-54 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Clarke JD, Hsu A, Yu Z, Dashwood RH, Ho E. 
In:
Mol Nutr Food Res (2011) 55(7): 999-1009 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kim JH, Dhanasekaran SM, Prensner JR, Cao X, Robinson D, Kalyana-Sundaram S, Huang C, Shankar S, Jing X, Iyer M, Hu M, Sam L, Grasso C, Maher CA, Palanisamy N, Mehra R, Kominsky HD, Siddiqui J, Yu J, Qin ZS, Chinnaiyan AM. 
In:
Genome Res (2011) 21(7): 1028-41 
Categories:
Primary Cells and Media 
Authors:
Prensner JR, Iyer MK, Balbin OA, Dhanasekaran SM, Cao Q, Brenner JC, Laxman B, Asangani IA, Grasso CS, Kominsky HD, Cao X, Jing X, Wang X, Siddiqui J, Wei JT, Robinson D, Iyer HK, Palanisamy N, Maher CA, Chinnaiyan AM. 
In:
Nat Biotechnol (2011) 29(8): 742-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Vo BT1, Khan SA. 
In:
Prostate (2011) 71(10): 1084-96 
Categories:
Primary Cells and Media 
Authors:
Basu A, Banerjee H, Rojas H, Martinez SR, Roy S, Jia Z, Lilly MB, De León M, Casiano CA. 
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Prostate (2011) 71(7): 755-65 
Categories:
Primary Cells and Media 
Authors:
Wang X1, Raulji P, Mohapatra SS, Patel R, Hellermann G, Kong X, Vera PL, Meyer-Siegler KL, Coppola D, Mohapatra S. 
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Mol Cancer (2011) 10:56: 1476-4598 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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Prostate (2011) 71(4): 333-43 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sekine Y, Osei-Hwedieh D, Matsuda K, Raghavachari N, Liu D, Furuya Y, Koike H, Suzuki K, Remaley AT. 
In:
Prostate (2011) doi: 10: 1002 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Xiao D1, Zeng Y, Prakash L, Badmaev V, Majeed M, Singh SV. 
In:
Mol Pharmacol (2011) 79(3): 499-507 
Categories:
Primary Cells and Media 
Authors:
Solier S1, Pommier Y. 
In:
Cancer Res (2011) 71(3): 906-13 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chaiswing L, Zhong W, Oberley TD. 
In:
Cancers (2011) 3(3): 3557-3584 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Leshem O, Madar S, Kogan-Sakin I, Kamer I, Goldstein I, Brosh R, Cohen Y, Jacob-Hirsch J, Ehrlich M, Ben-Sasson S, Goldfinger N, Loewenthal R, Gazit E, Rotter V, Berger R. 
In:
PLoS ONE (2011) 6(7): 21650 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Place TL, Fitzgerald MP, Venkataraman S, Vorrink SU, Case AJ, Teoh ML, Domann FE. 
In:
PLoS ONE (2011) 6(1): 14617 
Categories:
Primary Cells and Media 
Authors:
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In:
Nat Commun. (2011) 2: 162 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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PLoS ONE (2011) 6(10): 26595 
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PLoS ONE (2011) 6(9): 25187 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhang H1, Gogada R, Yadav N, Lella RK, Badeaux M, Ayres M, Gandhi V, Tang DG, Chandra D. 
In:
PLoS ONE (2011) 6(1): e16379 
Categories:
Primary Cells and Media, Transfection 
Authors:
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Cancer Res (2010) 70(23): 9544-9548 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Shan W, Zhong W, Zhao R, Oberley TD. 
In:
Free Radic Biol Med (2010) 49(12): 2078-87 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Theodore SC1, Rhim JS, Turner T, Yates C. 
In:
Other (2010) 20(1 Suppl 1: 96-100 
Categories:
Primary Cells and Media 
Authors:
Yu J1, Cao Q, Yu J, Wu L, Dallol A, Li J, Chen G, Grasso C, Cao X, Lonigro RJ, Varambally S, Mehra R, Palanisamy N, Wu JY, Latif F, Chinnaiyan AM. 
In:
Oncogene (2010) 29(39): 5370-80 
Categories:
Primary Cells and Media 
Authors:
Bhosle S, Suppiah S, Molinaro R, Liang Y, Arnold R, Diehl W, Makarova N, Blackwell J, Petros J, Liotta D, Hunter E, Ly H. 
In:
J Virol (2010) 84(13): 6288-96 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Healey MA, Deaton SL, Alder JK, Winnepenninckx V, Casero RA Jr, Herman JG. 
In:
Epigenetics (2010) 5(5): 410-21 
Categories:
Primary Cells and Media 
Authors:
Fitchev PP, Wcislak SM, Lee C, Bergh A, Brendler CB, Stellmach VM, Crawford SE, Mavroudis CD, Cornwell ML, Doll JA. 
In:
Lab Invest (2010) 90(7): 1078-90 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lee MY, Lu A, Gudas LJ. 
In:
J Cell Physiol (2010) 224(1): 17-27 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Heemers HV, Schmidt LJ, Kidd E, Raclaw KA, Regan KM, Tindall DJ. 
In:
Prostate (2010) 70(9): 959-70 
Categories:
Primary Cells and Media 
Authors:
Pandey M, Shukla S, Gupta S. 
In:
Int J Cancer (2010) 126(11): 2520-33 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sauer L, Gitenay D, Vo C, Baron VT. 
In:
Oncogene (2010) 29(18): 2628-37 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sun Y1, St Clair DK, Xu Y, Crooks PA, St Clair WH. 
In:
Cancer Res (2010) 70(7): 2880-90 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Romero D, Terzic A, Conley BA, Craft CS, Jovanovic B, Bergan RC, Vary CP 
In:
Carcinogenesis (2010) 31(3): 359-66 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Murakami M, Ugai H, Belousova N, Pereboev A, Dent P, Fisher PB, Everts M, Curiel DT. 
In:
Prostate (2010) 70(4): 362-76 
Categories:
Primary Cells and Media 
Authors:
Zhang P1, Singh A, Yegnasubramanian S, Esopi D, Kombairaju P, Bodas M, Wu H, Bova SG, Biswal S. 
In:
Mol Cancer Ther (2010) 9(2): 336-46 
Categories:
Primary Cells and Media 
Authors:
Oberg D, Yanover E, Adam V, Sweeney K, Costas C, Lemoine NR, Halldén G. 
In:
Clin Cancer Res (2010) 16(2): 541-53 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Krycer JR, Kristiana I, Brown AJ. 
In:
PLoS ONE (2009) 4(12): 8496 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lin C, Yang L, Tanasa B, Hutt K, Ju BG, Ohgi K, Zhang J, Rose DW, Fu XD, Glass CK, Rosenfeld MG. 
In:
Cell (2009) 139(6): 1069-83 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lagisetti C, Pourpak A, Goronga T, Jiang Q, Cui X, Hyle J, Lahti JM, Morris SW, Webb TR. 
In:
J Med Chem (2009) 52(22): 6979-90 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hsieh TC. 
In:
Asian J Androl (2009) 11(6): 653-61 
Categories:
Primary Cells and Media 
Authors:
Huang PH, Wang D, Chuang HC, Wei S, Kulp SK, Chen CS. 
In:
Carcinogenesis (2009) 30(7): 1125-31 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hong S, Klein EA, Das Gupta J, Hanke K, Weight CJ, Nguyen C, Gaughan C, Kim KA, Bannert N, Kirchhoff F, Munch J, Silverman RH. 
In:
J Virol (2009) 83(14): 6995-7003 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Abend JR, Joseph AE, Das D, Campbell-Cecen DB, Imperiale MJ. 
In:
J Gen Virol (2009) 90(Pt 5): 1238-45 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schauer IG, Ressler SJ, Rowley DR. 
In:
Prostate (2009) 69(4): 373-84 
Categories:
Primary Cells and Media 
Authors:
Solier S1, Sordet O, Kohn KW, Pommier Y. 
In:
Mol Cell Biol (2009) 29(1): 68-82 
Categories:
Primary Cells and Media 
Authors:
Dai Y, Liu M, Tang W, DeSano J, Burstein E, Davis M, Pienta K, Lawrence T, Xu L. 
In:
Clin Cancer Res (2008) 14(23): 7701-10 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Dillard PR, Lin MF, Khan SA. 
In:
Mol Endocrinol (2008) 295(1-2): 115-20 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Floryk D, Thompson TC. 
In:
Int J Cancer (2008) 123(10): 2294-302 
Categories:
Primary Cells and Media 
Authors:
Wang J1, Cai Y, Yu W, Ren C, Spencer DM, Ittmann M. 
In:
Cancer Res (2008) 68(20): 8516-24 
Categories:
Transfection 
Authors:
Zhang L, Smit-McBride Z, Pan X, Rheinhardt J, Hershey JW 
In:
J Biol Chem (2008) epub: online 
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