Endothelial, MV lung, human (HMVEC-L)

Endothelial cells line the inside surface of blood vessels, heart, lymphatic vessels, body cavities and other organs, of normal human tissue.

Small vessel endothelial cells are isolated from lung microvascular tissue.

Cell Type:
Microvascular Endo.
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

EGM-2 MV has been developed for the enhanced growth of lung microvascular endothelial cells
Can be used to grow all Clonetics Endothelial cells except HUVEC
Final serum concentration is 5%
Does not contain BBE

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P5

FP-100

1e5 79% 48% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P5

96-FP-100

1e5 75-83% 43-53% Plasmid (general) 0.4 µg 20 µl Shuttle
HMVEC-L S-005 5e5 39-65% 43-61% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (71)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chiang CF, Albarino CG, Lo MK, Spiropoulou CF. 
In:
PLoS ONE (2014) 9(6): e99764 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Birukova AA, Singleton PA, Gawlak G, Tian X, Mirzapoiazova T, Mambetsariev B, Dubrovskyi O, Oskolkova OV, Bochkov VN, Birukov KG. 
In:
Mol Biol Cell (2014) mbc.E13: 12-0743 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mambetsariev I, Tian Y, Wu T, Lavoie T, Solway J, Birukov KG, Birukova AA. 
In:
PLoS ONE (2014) 9(4): e92670 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Michalik KM, You X, Manavski Y, Doddaballapur A, Zörnig M, Braun T, John D, Ponomareva Y, Chen W, Uchida S, Boon RA, Dimmeler S. 
In:
Circ Res (2014) 114(9): 1389-97 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Star GP, Giovinazzo M, Lamoureux E, Langleben D 
In:
Other (2014) 14: 00289-6 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chen F, Yu Y, Haigh S, Johnson J, Lucas R, Stepp DW, Fulton DJ. 
In:
PLoS ONE (2014) 9(2): e88405 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Speyer CL1, Hachem AH2, Assi AA2, Johnson JS3, DeVries JA4, Gorski DH5. 
In:
PLoS ONE (2014) 9(3):: e88830. 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Roberts LL, Robinson CM. 
In:
Immunology (2014) 141(1): 39 - 51 
Categories:
Primary Cells and Media 
Authors:
Yu HJ1, Serebryannyy LA, Fry M, Greene M, Chernaya O, Hu WY, Chew TL, Mahmud N, Kadkol SS, Glover S, Prins G, Strakova Z, de Lanerolle P 
In:
PLoS ONE (2013) 8(11): e79776 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Penton CM, Thomas-Ahner JM, Johnson EK, McAllister C, Montanaro F 
In:
PLoS ONE (2013) 08(1): e54553 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Petrache I, Kamocki K, Poirier C, Pewzner-Jung Y, Laviad EL, Schweitzer KS, Van Demark M, Justice MJ, Hubbard WC, Futerman AH. 
In:
PLoS ONE (2013) 8(5): e62968 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Petrache I, Kamocki K, Poirier C, Pewzner-Jung Y, Laviad EL, Schweitzer KS, Van Demark M, Justice MJ, Hubbard WC, Futerman AH 
In:
PLoS ONE (2013) 8(5): e62968 
Categories:
Primary Cells and Media 
Authors:
Dong L, Li Z, Leffler NR, Asch AS, Chi JT, Yang LV. 
In:
PLoS ONE (2013) 8(4): e61991 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pavlova NN, Pallasch C, Elia AE, Braun CJ, Westbrook TF, Hemann M, Elledge SJ. 
In:
eLife (2013) 2: e00358 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Daniel A. Dworkis1,2, Elizabeth S. Klings1, Sherene M. Shenouda, Nadia Solovieff, Efthymia Melista, Carl Giovannucci, Surinder Safaya, Guihua Li, Joseph A. Vita, Martin H. Steinberg, Clinton T. Baldwin 
In:
Open Journal of Blood Diseases (2013) 3: 25-31 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sun S, Sursal T, Adibnia Y, Zhao C, Zheng Y, Li H, Otterbein LE, Hauser CJ, Itagaki K. 
In:
PLoS ONE (2013) 8(3): e59989 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
McNulty S, Flint M, Nichol ST, Spiropoulou CF. 
In:
J Virol (2013) 87(2): 912-22 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Profirovic J, Strekalova E, Urao N, Krbanjevic A, Andreeva AV, Varadarajan S, Fukai T, Hen R, Ushio-Fukai M, Voyno-Yasenetskaya TA 
In:
Angiogenesis (2013) 16(1): 15-28 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ivarsson M, Schollin J, Björkqvist M 
In:
Acta Paediatr. (2012) 102(10): 1010-6 
Categories:
Primary Cells and Media 
Authors:
Shimizu Y, Shibata R, Shintani S, Ishii M, Murohara T 
In:
J Am Heart Assoc (2012) 1(4): e000877 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gavrilovskaya IN, Gorbunova EE, Mackow ER 
In:
J Virol (2012) 86(16): 8765-72 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cross AS, Hyun SW, Miranda-Ribera A, Feng C, Liu A, Nguyen C, Zhang L, Luzina IG, Atamas SP, Twaddell WS, Guang W, Lillehoj EP, Puché AC, Huang W, Wang LX, Passaniti A, Goldblum SE. 
In:
J Biol Chem (2012) 287(19): 15966-80 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu A, Gong P, Hyun SW, Wang KZ, Cates EA, Perkins D, Bannerman DD, Puché AC, Toshchakov VY, Fang S, Auron PE, Vogel SN, Goldblum SE. 
In:
J Biol Chem (2012) 287(20): 16132-45 
Categories:
 
Authors:
Shu Q, Amin MA, Ruth JH, Campbell PL, Koch AE. 
In:
Arthritis Res Ther (2012) 14(2): R88 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yuan L, Le Bras A, Sacharidou A, Itagaki K, Zhan Y, Kondo M, Carman CV, Davis GE, Aird WC, Oettgen P 
In:
J Biol Chem (2012) 287(9): 6582-91 
Categories:
Primary Cells and Media 
Authors:
Thomas KC, Roberts JK, Deering-Rice CE, Romero EG, Dull RO, Lee J, Yost GS, Reilly CA. 
In:
Am J Physiol Lung Cell Mol Physiol (2012) 302(1):: L111-9 
Categories:
Primary Cells and Media 
Authors:
Gatheral T, Reed DM, Moreno L, Gough PJ, Votta BJ, Sehon CA, Rickard DJ, Bertin J, Lim E, Nicholson AG, Mitchell JA. 
In:
PLoS ONE (2012) 7(8): e42386 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sharma J, Young DM, Marentette JO, Rastogi P, Turk J, McHowat J. 
In:
Am J Physiol Lung Cell Mol Physiol (2012) 301(1): L47-55 
Categories:
 
Authors:
Yoo YA1, Kang MH, Lee HJ, Kim BH, Park JK, Kim HK, Kim JS, Oh SC. 
In:
Cancer Res (2011) 71(22): 7061-70 
Categories:
 
Authors:
Garg P, Yang S, Liu A, Pallero MA, Buchsbaum DJ, Mosher DF, Murphy-Ullrich JE, Goldblum SE 
In:
Am J Physiol Lung Cell Mol Physiol (2011) 301(1): L79-90 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kuznik A, Bencina M, Svajger U, Jeras M, Rozman B, Jerala R. 
In:
J Immunol (2011) 186(8): 4794-804 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sharma-Walia N, George Paul A, Patel K, Chandran K, Ahmad W, Chandran B 
In:
J Virol (2010) 84(24): 12733-53 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yagami A, Orihara K, Morita H, Futamura K, Hashimoto N, Matsumoto K, Saito H, Matsuda A. 
In:
J Immunol (2010) 185(10): 5743-50 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hermans K, Claes F, Vandevelde W, Zheng W, Geudens I, Orsenigo F, De Smet F, Gjini E, Anthonis K, Ren B, Kerjaschki D, Autiero M, Ny A, Simons M, Dewerchin M, Schulte-Merker S, Dejana E, Alitalo K, Carmeliet P. 
In:
Blood (2010) 116(17): 3356-66 
Categories:
 
Authors:
Drake JM, Barnes JM, Madsen JM, Domann FE, Stipp CS, Henry MD. 
In:
J Biol Chem (2010) 285(44): 33940-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ngamsri KC, Wagner R, Vollmer I, Stark S, Reutershan J 
In:
J Immunol (2010) 185(7): 4374-84 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wagner R, Ngamsri KC, Stark S, Vollmer I, Reutershan J. 
In:
Am J Physiol Lung Cell Mol Physiol (2010) 299(4): L502-12 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mirza MK, Sun Y, Zhao YD, Potula HH, Frey RS, Vogel SM, Malik AB, Zhao YY. 
In:
J Exp Med (2010) 207(8): 1675-85 
Categories:
 
Authors:
Larrieu-Lahargue F, Welm AL, Thomas KR, Li DY. 
In:
Blood (2010) 115(26): 5418-26 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Masuda M, Maruyama T, Ohta T, Ito A, Hayashi T, Tsukasaki K, Kamihira S, Yamaoka S, Hoshino H, Yoshida T, Watanabe T, Stanbridge EJ, Murakami Y. 
In:
J Biol Chem (2010) 285(20): 15511-22 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schingnitz U, Hartmann K, Macmanus CF, Eckle T, Zug S, Colgan SP, Eltzschig HK. 
In:
J Immunol (2010) 184(9): 5271-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Vandercappellen J, Liekens S, Bronckaers A, Noppen S, Ronsse I, Dillen C, Belleri M, Mitola S, Proost P, Presta M, Struyf S, Van Damme J. 
In:
Mol Cancer Res (2010) 8(3): 322-34 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Decaris ML, Lee CI, Yoder MC, Tarantal AF, Leach JK. 
In:
Angiogenesis (2009) 12(4): 303-11 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sverdlov M, Shinin V, Place AT, Castellon M, Minshall RD. 
In:
Mol Biol Cell (2009) 20(21): 4531-40 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pfeil U, Aslam M, Paddenberg R, Quanz K, Chang CL, Park JI, Gries B, Rafiq A, Faulhammer P, Goldenberg A, Papadakis T, Noll T, Hsu SY, Weissmann N, Kummer W. 
In:
Am J Physiol Lung Cell Mol Physiol (2009) 297(5): L837-45 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jin N, Cho SN, Raso MG, Wistuba I, Smith Y, Yang Y, Kurie JM, Yen R, Evans CM, Ludwig T, Jeong JW, DeMayo FJ. 
In:
Development (2009) 136(19): 3347-56 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Takagi Y, Hashimoto N, Phan SH, Imaizumi K, Matsuo M, Nakashima H, Hashimoto I, Hayashi Y, Kawabe T, Shimokata K, Hasegawa Y. 
In:
Am J Physiol Lung Cell Mol Physiol (2009) 297(3): L420-31 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kolliputi N, Waxman AB. 
In:
Am J Physiol Lung Cell Mol Physiol (2009) 297(1): L6-16 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kawanami D, Mahabeleshwar GH, Lin Z, Atkins GB, Hamik A, Haldar SM, Maemura K, Lamanna JC, Jain MK. 
In:
J Biol Chem (2009) 284(31): 20522-30 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Okazaki T, Ni A, Ayeni OA, Baluk P, Yao LC, Vossmeyer D, Zischinsky G, Zahn G, Knolle J, Christner C, McDonald DM. 
In:
Am J Pathol (2009) 174(6): 2378-87 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Minegishi Y, Saito M, Nagasawa M, Takada H, Hara T, Tsuchiya S, Agematsu K, Yamada M, Kawamura N, Ariga T, Tsuge I, Karasuyama H. 
In:
J Exp Med (2009) 206(6): 1291-301 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Raghu H, Sharma-Walia N, Veettil MV, Sadagopan S, Chandran B. 
In:
J Virol (2009) 83(10): 4895-911 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Drake JM, Strohbehn G, Bair TB, Moreland JG, Henry MD. 
In:
Mol Biol Cell (2009) 20(8): 2207-17 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hao Q, Wang L, Zhao ZJ, Tang H. 
In:
J Biol Chem (2009) 284(2): 799-806 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Aleku M, Schulz P, Keil O, Santel A, Schaeper U, Dieckhoff B, Janke O, Endruschat J, Durieux B, Röder N, Löffler K, Lange C, Fechtner M, Möpert K, Fisch G, Dames S, Arnold W, Jochims K, Giese K, Wiedenmann B, Scholz A, Kaufmann J. 
In:
Cancer Res (2008) 68(23): 9788-98 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Costello CM, Howell K, Cahill E, McBryan J, Konigshoff M, Eickelberg O, Gaine S, Martin F, McLoughlin P. 
In:
Am J Physiol Lung Cell Mol Physiol (2008) 295(2): L272-84 
Categories:
 
Authors:
Padera TP, Kuo AH, Hoshida T, Liao S, Lobo J, Kozak KR, Fukumura D, Jain RK. 
In:
Mol Cancer Ther (2008) 7(8): 2272-9 
Categories:
 
Authors:
Carla Danussi, Paola Spessotto, Alessandra Petrucco, Bruna Wassermann, Patrizia Sabatelli, Monica Montesi, Roberto Doliana, Giorgio M. Bressan, and Alfonso Colombatti 
In:
Mol Cell Biol (2008) 28(12): 4026-39 
Categories:
 
Authors:
Mikhaylova M, Mori N, Wildes FB, Walczak P, Gimi B, Bhujwalla ZM 
In:
Neoplasia (2008) 10(4): 380-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA 
In:
J Immunol (2008) 1;180(5): 3399-405 
Categories:
Transfection 
Authors:
Kapoor M, Zhou Q, Otero F, Myers CA, Bates A, Belani R, Liu J, Luo JK, Tzima E, Zhang DE, Yang XL, Schimmel P 
In:
J Biol Chem (2008) 283(4): 2070-7 
Categories:
Transfection 
Authors:
Zhang Y, Brovkovych V, Brovkovych S, Tan F, Lee BS, Sharma T, Skidgel RA 
In:
J Biol Chem (2007) 282(44): 32453-61 
Categories:
Primary Cells and Media 
Authors:
Dietrich T, Onderka J, Bock F, Kruse FE, Vossmeyer D, Stragies R, Zahn G, Cursiefen C 
In:
Am J Pathol (2007) 171(1): 361-72 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Reynolds AM, Xia W, Holmes MD, Hodge SJ, Danilov S, Curiel DT, Morrell NW, Reynolds PN 
In:
Am J Physiol Lung Cell Mol Physiol (2007) 292(5): L1182-92 
Categories:
Transfection 
Authors:
Laramee M, Chabot C, Cloutier M, Stenne R, Holgado-Madruga M, Wong AJ, Royal I 
In:
J Biol Chem (2007) 282(11): 7758-69 
Categories:
Transfection 
Authors:
Ralph JA, Zocco D, Bresnihan B, Fitzgerald O, McEvoy AN, Murphy EP 
In:
Am J Pathol (2007) 170(3): 1121-33 
Categories:
Transfection 
Authors:
Favier B, Alam A, Barron P, Bonnin J, Laboudie P, Fons P, Mandron M, Herault JP, Neufeld G, Savi P, Herbert JM, Bono F 
In:
Blood (2006) 108(4): 1243-50 
Categories:
Transfection 
Authors:
Ye SQ, Simon BA, Maloney JP, Zambelli-Weiner A, Gao L, Grant A, Easley RB, McVerry BJ, Tuder RM, Standiford T, Brower R, Barnes KC and Garcia JG 
In:
Am J Respir Crit Care Med (2005) 171(4): 361-370 
Categories:
Transfection 
Authors:
Stan RV, Tkachenko E and Niesman IR 
In:
Mol Biol Cell (2004) 15(8): 3615-3630 
Categories:
Primary Cells and Media 
Authors:
Chi JT, Chang HY, Haraldsen G, Jahnsen FL, Troyanskaya OG, Chang DS, Wang Z, Rockson SG, van de Rijn M, Botstein D, Brown PO. 
In:
Proc Natl Acad Sci USA (2003) 100 (19): 10623-8 
+ Show All