Endothelial, coronary art, human (HCAEC)

Primary endothelial cells

Cell Type:
Coronary Artery Endo.
Tissue Origin:
artery
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

EGM-2 MV has been developed for the enhanced growth of lung microvascular endothelial cells
Can be used to grow all Clonetics Endothelial cells except HUVEC
Final serum concentration is 5%
Does not contain BBE

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
HCAEC S-005 5e5 57% ±11 42% ±8 Plasmid (general) 5 µg 100 µl I/II/2b

Citations (35)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tsukahara T, Tsukahara R, Haniu H, Matsuda Y, Murakami-Murofushi K 
In:
Mol Endocrinol (2015) 412: 320-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu Y, Xie A, Singh AK, Ehsan A, Choudhary G, Dudley S, Sellke FW, Feng J 
In:
J Am Heart Assoc (2015) 4(8): e002062 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Michalik KM, You X, Manavski Y, Doddaballapur A, Zörnig M, Braun T, John D, Ponomareva Y, Chen W, Uchida S, Boon RA, Dimmeler S. 
In:
Circ Res (2014) 114(9): 1389-97 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
dela Paz NG, Melchior B, Shayo FY, Frangos JA. 
In:
J Biol Chem (2014) 289(11): 7413-24 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wang T, Green LA, Gupta SK, Kim C, Wang L, Almodovar S, Flores SC, Prudovsky IA, Jolicoeur P, Liu Z, Clauss M 
In:
PLoS ONE (2014) 9(3): e91063 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Masataka Kudo, Ken Matsuda, Kaori Sugawara, Yuko Iki, Naotaka Kogure, Takako Saito-Ito, Kyoko Shimizu, Ikuko Sato, Takeo Yoshikawa, Akira Uruno, Ryo Ito, Atsushi Yokoyama, Akiko Saito-Hakoda, Sadayoshi Ito, Akira Sugawara 
In:
Other (2014) 4(1): 7-14 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Linnéa Eriksson 
In:
Other (2014) 1: 1-66 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Menghini R1, Casagrande V1, Marino A1, Marchetti V1, Cardellini M1, Stoehr R1, Rizza S1, Martelli E2, Greco S3, Mauriello A2, Ippoliti A2, Martelli F3, Lauro R1, Federici M1. 
In:
Cell Death Dis. (2014) 5: e1029 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nishijima Y, Zheng X, Lund H, Suzuki M, Mattson DL, Zhang DX 
In:
Other (2014) 2(1): e00199 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sharma J, Eickhoff CS, Hoft DF, Marentette JO, Turk J, McHowat J. 
In:
Other (2014) 2(1): e00196 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Agarwal B, Campen MJ, Channell MM, Wherry SJ, Varamini B, Davis JG, Baur JA, Smoliga JM. 
In:
Int J Cardiol (2013) 166(1): 246-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Donovan EL, Pettine SM, Hickey MS, Hamilton KL, Miller BF. 
In:
Diabetol Metab Syndr (2013) 5(1): 24 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Stavik B, Tinholt M, Sletten M, Skretting G, Sandset PM, Iversen N. 
In:
J Hematol Oncol (2013) 6: 5 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Young Park J, Wook Yun J, Whan Choi Y, Ung Bae J, Won Seo K, Jin Lee S, Youn Park S, Whan Hong K, Kim CD. 
In:
Hypertens Res (2012) 35(9):: 928-34 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Queiroz KC, Bijlsma MF, Tio RA, Zeebregts CJ, Dunaeva M, Ferreira CV, Fuhler GM, Kuipers EJ, Alves MM, Rezaee F, Spek CA, Peppelenbosch MP. 
In:
Mol Med (2012) 18: 1122-7 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Shaw JH, Lloyd PG 
In:
Microvasc Res (2012) 84(2): 155-60 
Categories:
Primary Cells and Media 
Authors:
Nabzdyk CS, Chun M, Pradhan Nabzdyk L, Yoshida S, LoGerfo FW 
In:
Biochim Biophys Acta (2012) 425(2): 261-5 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Palayoor ST, J-Aryankalayil M, Makinde AY, Cerna D, Falduto MT, Magnuson SR, Coleman CN. 
In:
J Cardiovasc Pharmacol (2012) 59(6): 487-99 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Channell MM, Paffett ML, Devlin RB, Madden MC, Campen MJ. 
In:
Toxicol Sci (2012) 127(1): 179-86 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Eriksson L, Erdogdu O, Nyström T, Zhang Q, Sjöholm Å. 
In:
Cardiovasc Diabetol (2012) 11: 27 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Birukova AA, Lee S, Starosta V, Wu T, Ho T, Kim J, Berliner JA, Birukov KG. 
In:
PLoS ONE (2012) 1: e30957 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Curatola AM, Huang K, Naftolin F. 
In:
Regenerative Med (2012) 19(1): 86-91 
Categories:
Primary Cells and Media 
Authors:
Donovan EL, McCord JM, Reuland DJ, Miller BF, Hamilton KL. 
In:
Oxid Med Cell Longev (2012) 2012: 132931 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
El-Gamal D, Holzer M, Gauster M, Schicho R, Binder V, Konya V, Wadsack C, Schuligoi R, Heinemann A, Marsche G. 
In:
Antioxid Redox Signal (2012) 16(2): 129-37 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Larsson P, Ulfhammer E, Magnusson M, Bergh N, Lunke S, El-Osta A, Medcalf RL, Svensson PA, Karlsson L, Jern S. 
In:
PLoS ONE (2012) 7(2): e31573 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yagami A, Orihara K, Morita H, Futamura K, Hashimoto N, Matsumoto K, Saito H, Matsuda A. 
In:
J Immunol (2010) 185(10): 5743-50 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sinclair AM, Coxon A, McCaffery I, Kaufman S, Paweletz K, Liu L, Busse L, Swift S, Elliott S, Begley CG. 
In:
Blood (2010) 115(21): 4264-72 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Menghini R, Casagrande V, Cardellini M, Martelli E, Terrinoni A, Amati F, Vasa-Nicotera M, Ippoliti A, Novelli G, Melino G, Lauro R, Federici M. 
In:
Circulation (2009) 120(15): 1524-32 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hao Q, Wang L, Zhao ZJ, Tang H. 
In:
J Biol Chem (2009) 284(2): 799-806 
Categories:
Transfection 
Authors:
Pleger ST, Harris DM, Shan C, Vinge LE, Chuprun JK, Berzins B, Pleger W, Druckman C, Völkers M, Heierhorst J, Oie E, Remppis A, Katus HA, Scalia R, Eckhart AD, Koch WJ, Most P 
In:
Circ Res (2008) 102(7): 786-94 
Categories:
Transfection 
Authors:
Csiszar A, Ahmad M, Smith KE, Labinskyy N, Gao Q, Kaley G, Edwards JG, Wolin MS and Ungvari Z 
In:
Am J Pathol (2006) 168(2): 629-638 
Categories:
Transfection 
Authors:
Iversen N, Birkenes B, Torsdalen K and Djurovic S 
In:
Genet Vaccines Ther (2005) 3(1): 2 
Categories:
Primary Cells and Media 
Authors:
Chi JT, Chang HY, Haraldsen G, Jahnsen FL, Troyanskaya OG, Chang DS, Wang Z, Rockson SG, van de Rijn M, Botstein D, Brown PO. 
In:
Proc Natl Acad Sci USA (2003) 100 (19): 10623-8 
Categories:
Primary Cells and Media 
Authors:
Codina-Fauteux VA1,2, Beaudoin M1, Lalonde S1, Lo KS1, Lettre G3,4. 
In:
BMC Genet () 1: 97 
Categories:
Primary Cells and Media 
Authors:
Wagner JUG1,2, Chavakis E3, Rogg EM1, Muhly-Reinholz M1, Glaser SF1,2, Günther S4, John D1, Bonini F1, Zeiher AM3,2, Schaefer L5, Hannocks MJ2, Boon RA1,2, Dimmeler S6,2. 
In:
Arterioscler Thromb Vasc Biol () 38: 1170-1177 
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