Endothelial, coronary art, human (HCAEC)

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Primary endothelial cells

Cell Type:
Coronary Artery Endo.
Tissue Origin:
artery
Species:
human
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Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

Endothelial Cell Growth Medium-2 Microvascular

EGM-2 MV has been developed for the enhanced growth of lung microvascular endothelial cells
Can be used to grow all Clonetics Endothelial cells except HUVEC
Final serum concentration is 5%
Does not contain BBE

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
HCAEC S-005 5e5 57% ±11 42% ±8 Plasmid (general) 5 µg 100 µl I/II/2b

Citations (35)

Switch in Laminin ß2 to Laminin ß1 Isoforms During Aging Controls Endothelial Cell Functions-Brief Report
Categories:
Primary Cells and Media 
Authors:
Wagner JUG1,2, Chavakis E3, Rogg EM1, Muhly-Reinholz M1, Glaser SF1,2, Günther S4, John D1, Bonini F1, Zeiher AM3,2, Schaefer L5, Hannocks MJ2, Boon RA1,2, Dimmeler S6,2. 
In:
Arterioscler Thromb Vasc Biol (2018) 38: 1170-1177 
Cyclic phosphatidic acid inhibits the secretion of vascular endothelial growth factor from diabetic human coronary artery endothelial cells through peroxisome proliferator-activated receptor gamma
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tsukahara T, Tsukahara R, Haniu H, Matsuda Y, Murakami-Murofushi K 
In:
Mol Endocrinol (2015) 412: 320-9 
Inactivation of Endothelial Small/Intermediate Conductance of Calcium-Activated Potassium Channels Contributes to Coronary Arteriolar Dysfunction in Diabetic Patients
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu Y, Xie A, Singh AK, Ehsan A, Choudhary G, Dudley S, Sellke FW, Feng J 
In:
J Am Heart Assoc (2015) 4(8): e002062 
Long noncoding RNA MALAT1 regulates endothelial cell function and vessel growth.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Michalik KM, You X, Manavski Y, Doddaballapur A, Zörnig M, Braun T, John D, Ponomareva Y, Chen W, Uchida S, Boon RA, Dimmeler S. 
In:
Circ Res (2014) 114(9): 1389-97 
Heparan sulfates mediate the interaction between platelet endothelial cell adhesion molecule-1 (PECAM-1) and the Gaq/11 subunits of heterotrimeric G proteins.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
dela Paz NG, Melchior B, Shayo FY, Frangos JA. 
In:
J Biol Chem (2014) 289(11): 7413-24 
Transfer of intracellular HIV Nef to endothelium causes endothelial dysfunction.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wang T, Green LA, Gupta SK, Kim C, Wang L, Almodovar S, Flores SC, Prudovsky IA, Jolicoeur P, Liu Z, Clauss M 
In:
PLoS ONE (2014) 9(3): e91063 
ARB affects nicotine-induced gene expression profile in human coronary artery endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Masataka Kudo, Ken Matsuda, Kaori Sugawara, Yuko Iki, Naotaka Kogure, Takako Saito-Ito, Kyoko Shimizu, Ikuko Sato, Takeo Yoshikawa, Akira Uruno, Ryo Ito, Atsushi Yokoyama, Akiko Saito-Hakoda, Sadayoshi Ito, Akira Sugawara 
In:
Other (2014) 4(1): 7-14 
ON ANTIDIABETIC AGENTS AND THE VASCULATURE
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Linnéa Eriksson 
In:
Other (2014) 1: 1-66 
MiR-216a: a link between endothelial dysfunction and autophagy.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Menghini R1, Casagrande V1, Marino A1, Marchetti V1, Cardellini M1, Stoehr R1, Rizza S1, Martelli E2, Greco S3, Mauriello A2, Ippoliti A2, Martelli F3, Lauro R1, Federici M1. 
In:
Cell Death Dis. (2014) 5: e1029 
Characterization of blood pressure and endothelial function in TRPV4-deficient mice with l-NAME- and angiotensin II-induced hypertension.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nishijima Y, Zheng X, Lund H, Suzuki M, Mattson DL, Zhang DX 
In:
Other (2014) 2(1): e00199 
Absence of calcium-independent phospholipase A2 ß impairs platelet-activating factor production and inflammatory cell recruitment in Trypanosoma cruzi-infected endothelial cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sharma J, Eickhoff CS, Hoft DF, Marentette JO, Turk J, McHowat J. 
In:
Other (2014) 2(1): e00196 
Resveratrol for primary prevention of atherosclerosis: clinical trial evidence for improved gene expression in vascular endothelium.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Agarwal B, Campen MJ, Channell MM, Wherry SJ, Varamini B, Davis JG, Baur JA, Smoliga JM. 
In:
Int J Cardiol (2013) 166(1): 246-8 
Lipidomic analysis of human plasma reveals ether-linked lipids that are elevated in morbidly obese humans compared to lean
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Donovan EL, Pettine SM, Hickey MS, Hamilton KL, Miller BF. 
In:
Diabetol Metab Syndr (2013) 5(1): 24 
TFPIa and TFPIß are expressed at the surface of breast cancer cells and inhibit TF-FVIIa activity.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Stavik B, Tinholt M, Sletten M, Skretting G, Sandset PM, Iversen N. 
In:
J Hematol Oncol (2013) 6: 5 
Antihypertensive effect of gomisin A from Schisandra chinensis on angiotensin II-induced hypertension via preservation of nitric oxide bioavailability
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Young Park J, Wook Yun J, Whan Choi Y, Ung Bae J, Won Seo K, Jin Lee S, Youn Park S, Whan Hong K, Kim CD. 
In:
Hypertens Res (2012) 35(9):: 928-34 
Dichotomy in Hedgehog signaling between human healthy vessel and atherosclerotic plaques
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Queiroz KC, Bijlsma MF, Tio RA, Zeebregts CJ, Dunaeva M, Ferreira CV, Fuhler GM, Kuipers EJ, Alves MM, Rezaee F, Spek CA, Peppelenbosch MP. 
In:
Mol Med (2012) 18: 1122-7 
Post-transcriptional regulation of placenta growth factor mRNA by hydrogen peroxide
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Shaw JH, Lloyd PG 
In:
Microvasc Res (2012) 84(2): 155-60 
Differential susceptibility of human primary aortic and coronary artery vascular cells to RNA interference.
Categories:
Primary Cells and Media 
Authors:
Nabzdyk CS, Chun M, Pradhan Nabzdyk L, Yoshida S, LoGerfo FW 
In:
Biochim Biophys Acta (2012) 425(2): 261-5 
Gene expression profile of coronary artery cells treated with nonsteroidal anti-inflammatory drugs reveals off-target effects
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Palayoor ST, J-Aryankalayil M, Makinde AY, Cerna D, Falduto MT, Magnuson SR, Coleman CN. 
In:
J Cardiovasc Pharmacol (2012) 59(6): 487-99 
Circulating factors induce coronary endothelial cell activation following exposure to inhaled diesel exhaust and nitrogen dioxide in humans: evidence from a novel translational in vitro model
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Channell MM, Paffett ML, Devlin RB, Madden MC, Campen MJ. 
In:
Toxicol Sci (2012) 127(1): 179-86 
Effects of some anti-diabetic and cardioprotective agents on proliferation and apoptosis of human coronary artery endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Eriksson L, Erdogdu O, Nyström T, Zhang Q, Sjöholm Å. 
In:
Cardiovasc Diabetol (2012) 11: 27 
A role for VEGFR2 activation in endothelial responses caused by barrier disruptive OxPAPC concentrations.
Categories:
Primary Cells and Media 
Authors:
Birukova AA, Lee S, Starosta V, Wu T, Ho T, Kim J, Berliner JA, Birukov KG. 
In:
PLoS ONE (2012) 1: e30957 
Dehydroepiandrosterone (DHEA) inhibition of monocyte binding by vascular endothelium is associated with sialylation of neural cell adhesion molecule
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Curatola AM, Huang K, Naftolin F. 
In:
Regenerative Med (2012) 19(1): 86-91 
Phytochemical activation of Nrf2 protects human coronary artery endothelial cells against an oxidative challenge
Categories:
Primary Cells and Media 
Authors:
Donovan EL, McCord JM, Reuland DJ, Miller BF, Hamilton KL. 
In:
Oxid Med Cell Longev (2012) 2012: 132931 
Cyanate is a novel inducer of endothelial icam-1 expression
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
El-Gamal D, Holzer M, Gauster M, Schicho R, Binder V, Konya V, Wadsack C, Schuligoi R, Heinemann A, Marsche G. 
In:
Antioxid Redox Signal (2012) 16(2): 129-37 
Role of histone acetylation in the stimulatory effect of valproic acid on vascular endothelial tissue-type plasminogen activator expression
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Larsson P, Ulfhammer E, Magnusson M, Bergh N, Lunke S, El-Osta A, Medcalf RL, Svensson PA, Karlsson L, Jern S. 
In:
PLoS ONE (2012) 7(2): e31573 
IL-33 Mediates Inflammatory Responses in Human Lung Tissue Cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yagami A, Orihara K, Morita H, Futamura K, Hashimoto N, Matsumoto K, Saito H, Matsuda A. 
In:
J Immunol (2010) 185(10): 5743-50 
Functional erythropoietin receptor is undetectable in endothelial, cardiac, neuronal, and renal cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sinclair AM, Coxon A, McCaffery I, Kaufman S, Paweletz K, Liu L, Busse L, Swift S, Elliott S, Begley CG. 
In:
Blood (2010) 115(21): 4264-72 
MicroRNA 217 modulates endothelial cell senescence via silent information regulator 1.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Menghini R, Casagrande V, Cardellini M, Martelli E, Terrinoni A, Amati F, Vasa-Nicotera M, Ippoliti A, Novelli G, Melino G, Lauro R, Federici M. 
In:
Circulation (2009) 120(15): 1524-32 
Identification of protein kinase D2 as a pivotal regulator of endothelial cell proliferation, migration, and angiogenesis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hao Q, Wang L, Zhao ZJ, Tang H. 
In:
J Biol Chem (2009) 284(2): 799-806 
Endothelial S100A1 Modulates Vascular Function via Nitric Oxide
Categories:
Transfection 
Authors:
Pleger ST, Harris DM, Shan C, Vinge LE, Chuprun JK, Berzins B, Pleger W, Druckman C, Völkers M, Heierhorst J, Oie E, Remppis A, Katus HA, Scalia R, Eckhart AD, Koch WJ, Most P 
In:
Circ Res (2008) 102(7): 786-94 
Bone morphogenetic protein-2 induces proinflammatory endothelial phenotype
Categories:
Transfection 
Authors:
Csiszar A, Ahmad M, Smith KE, Labinskyy N, Gao Q, Kaley G, Edwards JG, Wolin MS and Ungvari Z 
In:
Am J Pathol (2006) 168(2): 629-638 
Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells
Categories:
Transfection 
Authors:
Iversen N, Birkenes B, Torsdalen K and Djurovic S 
In:
Genet Vaccines Ther (2005) 3(1): 2 
Endothelial cell diversity revealed by global expression profiling
Categories:
Primary Cells and Media 
Authors:
Chi JT, Chang HY, Haraldsen G, Jahnsen FL, Troyanskaya OG, Chang DS, Wang Z, Rockson SG, van de Rijn M, Botstein D, Brown PO. 
In:
Proc Natl Acad Sci USA (2003) 100 (19): 10623-8 
PHACTR1 splicing isoforms and eQTLs in atherosclerosis-relevant human cells.
Categories:
Primary Cells and Media 
Authors:
Codina-Fauteux VA1,2, Beaudoin M1, Lalonde S1, Lo KS1, Lettre G3,4. 
In:
BMC Genet () 1: 97 
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