SMC, aortic (AoSMC), human

Smooth muscle cells isoalted from human aorta.

Cell Type:
Smooth muscle cell
Tissue Origin:
aortic
Species:
human
Research Area:
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

 SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P1

EH-106

1e5 74% 92% Plasmid (general) 0.2 µg 20 µl 4D X-Unit
P1

EH-106

1e5 74% 92% Plasmid (general) 0.2 µg 20 µl Shuttle
AoSMC, human U-025 1e6 57-79% 90-96% Plasmid (general) 5 µg 100 µl I/II/2b
Smooth muscle, basic P-024 2e6 75% 69% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (37)

Categories:
Primary Cells and Media 
Authors:
Wongtrakool C1, Grooms K2, Bijli KM1, Crothers K3, Fitzpatrick AM4, Hart CM1 
In:
PLoS ONE (2014) 9(10): e109602 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Klein D, Benchellal M, Kleff V, Jakob HG, Ergün S 
In:
Scientific Reports (2013) 3: 2178 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shroff RC, Price KL, Kolatsi-Joannou M, Todd AF, Wells D, Deanfield J, Johnson RJ, Rees L, Woolf AS, Long DA 
In:
PLoS ONE (2013) 8(2): e56273 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Csányi G, Yao M, Rodríguez AI, Al Ghouleh I, Sharifi-Sanjani M, Frazziano G, Huang X, Kelley EE, Isenberg JS, Pagano PJ. 
In:
Arterioscler Thromb Vasc Biol (2012) 32(12): 2966-73 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Morimoto M, Yu Z, Stenzel P, Clewing JM, Najafian B, Mayfield C, Hendson G, Weinkauf JG, Gormley AK, Parham DM, Ponniah U, André JL, Asakura Y, Basiratnia M, Bogdanovic R, Bokenkamp A, Bonneau D, Buck A, Charrow J, Cochat P, Cordeiro I, Deschenes G, Fenkçi MS, Frange P, Fründ S, Fryssira H, Guillen-Navarro E, Keller K, Kirmani S, Kobelka C, Lamfers P, Levtchenko E, Lewis DB, Massella L, McLeod DR, Milford DV, Nobili F, Saraiva JM, Semerci CN, Shoemaker L, Stajic N, Stein A, Taha D, Wand D, Zonana J, Lücke T, Boerkoel CF. 
In:
Orphanet Journal of Rare Diseases (2012) 7: 70 
Categories:
Primary Cells and Media 
Authors:
Nabzdyk CS, Chun M, Pradhan Nabzdyk L, Yoshida S, LoGerfo FW 
In:
Biochim Biophys Acta (2012) 425(2): 261-5 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zouaoui Boudjeltia K, Daher J, Van Antwerpen P, Moguilevsky N, Delree P, Ducobu J, Raes M, Badran B, Vanhaeverbeek M, Brohee D, Remacle C, Vanhamme L. 
In:
PLoS ONE (2012) 7: 0038810 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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In:
Apoptosis (2012) ePub: e 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhao N, Liu H, Lilly B 
In:
J Biol Chem (2012) 287(20): 16111-20 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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In:
Cell Death Differ (2011) 2: e223 
Categories:
Primary Cells and Media 
Authors:
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In:
J Biol Chem (2011) 286(26): 23620 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rastogi S, Rizwani W, Joshi B, Kunigal S, Chellappan SP 
In:
Cell Death Differ (2011) 19(2): 274-83 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Willette RN, Bao W, Nerurkar S, Yue TL, Doe CP, Stankus G, Turner GH, Ju H, Thomas H, Fishman CE, Sulpizio A, Behm DJ, Hoffman S, Lin Z, Lozinskaya I, Casillas LN, Lin M, Trout RE, Votta BJ, Thorneloe K, Lashinger ES, Figueroa DJ, Marquis R, Xu X. 
In:
J Pharmacol Exp Ther (2011) 338(1): 408-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kotlarchyk MA, Shreim SG, Alvarez-Elizondo MB, Estrada LC, Singh R, Valdevit L, Kniazeva E, Gratton E, Putnam AJ, Botvinick EL 
In:
PLoS ONE (2011) 6(5): 1-12 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Leeper NJ, Raiesdana A, Kojima Y, Chun HJ, Azuma J, Maegdefessel L, Kundu RK, Quertermous T, Tsao PS, Spin JM 
In:
J Cell Physiol (2011) 226(4): 1035-43 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nabzdyk CS, Chun M, Pradhan L, Logerfo FW 
In:
J Transl Med (2011) 9(48): 1-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Vigetti D, Clerici M, Deleonibus S, Karousou E, Viola M, Moretto P, Heldin P, Hascall VC, De Luca G, Passi A 
In:
J Biol Chem (2011) 286(10): 7917-24 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Perez VA, Ali Z, Alastalo TP, Ikeno F, Sawada H, Lai YJ, Kleisli T, Spiekerkoetter E, Qu X, Rubinos LH, Ashley E, Amieva M, Dedhar S, Rabinovitch M 
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J Cell Biol (2011) 192(1): 171-88 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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In:
Am J Physiol Heart Circ Physiol (2010) 299(6): H2018-27 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shai SY, Sukhanov S, Higashi Y, Vaughn C, Kelly J, Delafontaine P 
In:
Arterioscler Thromb Vasc Biol (2010) 30(10): 1916-24 
Categories:
Primary Cells and Media 
Authors:
Montes de Oca A, Madueño JA, Martinez-Moreno JM, Guerrero F, Muñoz-Castañeda J, Rodriguez-Ortiz ME, Mendoza FJ, Almaden Y, Lopez I, Rodriguez M, Aguilera-Tejero E 
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Other (2010) 25(9): 1996-2005 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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In:
Am J Physiol Cell Physiol (2010) 299(3): C638-42 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Merfeld-Clauss S, Gollahalli N, March KL, Traktuev DO. 
In:
Tissue Eng (2010) 16(9): 2953-66 
Categories:
Primary Cells and Media 
Authors:
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Circulation (2010) 121(18): 2001-11 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Andukuri A, Minor WP, Kushwaha M, Anderson JM, Jun HW 
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Other (2010) 6(2): 289-97 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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Biomaterials (2010) 31(7): 1502-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Van Campenhout A, Moran CS, Parr A, Clancy P, Rush C, Jakubowski H, Golledge J 
In:
Other (2009) 202(2): 557-566 
Categories:
Transfection 
Authors:
Nieves-Cintron M, Amberg GC, Navedo MF, Molkentin JD, Santana LF 
In:
Proc Natl Acad Sci USA (2008) 105(40): 15623-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Isenberg BC, Tsuda Y, Williams C, Shimizu T, Yamato M, Okano T, Wong JY 
In:
Biomaterials (2008) 29(17): 2565-72 
Categories:
Transfection 
Authors:
Raman P, Krukovets I, Marinic TE, Bornstein P, Stenina OI 
In:
J Biol Chem (2007) 282(8): 5704-14 
Categories:
Transfection 
Authors:
Sukhanov S, Higashi Y, Shai SY, Itabe H, Ono K, Parthasarathy S, Delafontaine P 
In:
Circ Res (2006) 99(2): 191-200 
Categories:
Transfection 
Authors:
Vigetti D, Moretto P, Viola M, Genasetti A, Rizzi M, Karousou E, Pallotti F, De Luca G, Passi A 
In:
FASEB J (2006) 20(8): 1118-1130 
Categories:
Transfection 
Authors:
Reddy MA, Li SL, Sahar S, Kim YS, Xu ZG, Lanting L, Natarajan R 
In:
J Biol Chem (2006) 281(19): 13685-93 
Categories:
Transfection 
Authors:
Vigetti D, Ori M, Viola M, Genasetti A, Karousou E, Rizzi M, Pallotti F, Nardi I, Hascall VC, De Luca G and Passi A 
In:
J Biol Chem (2006) 281(12): 8254-8263 
Categories:
Transfection 
Authors:
Tomura H, Wang JQ, Komachi M, Damirin A, Mogi C, Tobo M, Kon J, Misawa N, Sato K and Okajima F 
In:
J Biol Chem (2005) 280(41): 34458-34464 
Categories:
Transfection 
Authors:
Iversen N, Birkenes B, Torsdalen K and Djurovic S 
In:
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