B cell, human

One of the two major classes of lymphocytes, B lymphocytes are white blood cells of the immune system that are derived from the bone marrow and spleen.

Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and human or recombinant transferrin.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
B cell, human U-015 1e6 25-47% 84-92% Plasmid (general) 5 µg 100 µl I/II/2b
P3 96-EO-117 1e6 22-34% 63-77% Plasmid (general) 0.4 µg 20 µl Shuttle
P3

EO-117

1e6 22-34% 63-77% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (26)

Categories:
Transfection 
Authors:
Hartweger H, McGuire AT, Horning M, Taylor JJ, Dosenovic P, Yost D, Gazumyan A, Seaman MS, Stamatatos L, Jankovic M, Nussenzweig MC. 
In:
J Exp Med (2019) 216 (6): 1301-1310 
Categories:
Primary Cells and Media, Transfection 
Authors:
Greiner V, Bou Puerto R, Liu S, Herbel C, Carmona EM, Goldberg MS. 
In:
iScience (2019) 12: 369-378 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Larman HB, Scott ER, Wogan M, Oliveira G, Torkamani A, Schultz PG 
In:
Nucleic Acids Res (2014) 42(4): 9146-57 
Categories:
Transfection 
Authors:
Rappocciolo G, Jais M, Piazza P, Reinhart TA, Berendam SJ, Garcia-Exposito L, Gupta P, Rinaldo CR 
In:
mBio (2014) 5(3): 1-11 
Categories:
Transfection 
Authors:
Snow AL, Xiao W, Stinson JR, Lu W, Chaigne-Delalande B, Zheng L, Pittaluga S, Matthews HF, Schmitz R, Jhavar S, Kuchen S, Kardava L, Wang W, Lamborn IT, Jing H, Raffeld M, Moir S, Fleisher TA, Staudt LM, Su HC, Lenardo MJ. 
In:
J Exp Med (2012) 209(12): 2247-2261 
Categories:
Transfection 
Authors:
Kardava L, Moir S, Wang W, Ho J, Buckner CM, Posada JG, O'Shea MA, Roby G, Chen J, Sohn HW, Chun TW, Pierce SK, Fauci AS. 
In:
J Clin Invest (2011) 121(7): 2614-24 
Categories:
Transfection 
Authors:
Garaud S, Le Dantec C, Berthou C, Lydyard PM, Youinou P, Renaudineau Y 
In:
J Immunol (2008) 181(3): 2010-8 
Categories:
Transfection 
Authors:
Mason NJ, Coughlin CM, Overley B, Cohen JN, Mitchell EL, Colligon TA, Clifford CA, Zurbriggen A, Sorenmo KU, Vonderheide RH 
In:
Gene Ther (2008) 15(13): 955-65 
Categories:
Transfection 
Authors:
Zhou HJ, Pham LV, Tamayo AT, Lin-Lee YC, Fu L, Yoshimura LC, Ford RJ 
In:
Blood (2007) 110(6): 2121-7 
Categories:
Transfection 
Authors:
Seiffert M, Stilgenbauer S, Döhner H, Lichter P 
In:
Leukemia (2007) 21(9): 1977-83 
Categories:
Transfection 
Authors:
Good KL, Tangye SG 
In:
Proc Natl Acad Sci USA (2007) 104(33): 13420-5 
Categories:
Transfection 
Authors:
Sakurai D, Hase H, Kanno Y, Kojima H, Okumura K, Kobata T 
In:
Blood (2007) 109(7): 2961-7 
Categories:
Transfection 
Authors:
Lin K, Glenn MA, Harris RJ, Duckworth AD, Dennett S, Cawley JC, Zuzel M, Slupsky JR 
In:
Cancer Res (2006) 66(15): 7801-9 
Categories:
Transfection 
Authors:
Lin-Lee YC, Pham LV, Tamayo AT, Fu L, Zhou HJ, Yoshimura LC, Decker GL, Ford RJ 
In:
J Biol Chem (2006) 281(27): 18878-87 
Categories:
Transfection 
Authors:
Sainz-Perez A, Gary-Gouy H, Portier A, Davi F, Merle-Beral H, Galanaud P, Dalloul A 
In:
Leukemia (2006) 20(3): 498-504 
Categories:
Transfection 
Authors:
Lee CH, Melchers M, Wang H, Torrey TA, Slota R, Qi CF, Kim JY, Lugar P, Kong HJ, Farrington L, van der Zouwen B, Zhou JX, Lougaris V, Lipsky PE, Grammer AC and Morse HC 3rd 
In:
J Exp Med (2006) 203(1): 63-72 
Categories:
Transfection 
Authors:
Pham LV, Tamayo AT, Yoshimura LC, Lin-Lee YC and Ford RJ 
In:
Blood (2005) 106(12): 3940-3947 
Categories:
Transfection 
Authors:
Roesler J, Izquierdo JM, Ryser M, Rosen-Wolff A, Gahr M, Valcarcel J, Lenardo MJ and Zheng L 
In:
Blood (2005) 106(5): 1652-1659 
Categories:
Transfection 
Authors:
Gorgun G, Holderried TA, Zahrieh D, Neuberg D and Gribben JG 
In:
J Clin Invest (2005) 115(7): 1797-1805 
Categories:
Transfection 
Authors:
Laichalk LL and Thorley-Lawson DA 
In:
J Virol (2005) 79(2): 1296-1307 
Categories:
Transfection 
Authors:
Van der Stoep N, Quinten E, Rezende MM and Van den Elsen PJ 
In:
Blood (2004) 104(9): 2849-2857 
Categories:
Transfection 
Authors:
Hofmann SR, Lam AQ, Frank S, Zhou YJ, Ramos HL, Kanno Y, Agnello D, Youle RJ and O'Shea JJ 
In:
Mol Cell Biol (2004) 24(11): 5039-5049 
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
Categories:
Transfection 
Authors:
Coughlin CM, Vance BA, Grupp SA and Vonderheide RH 
In:
Blood (2004) 103: 2046-2054 
Categories:
Transfection 
Authors:
Tolnay M, Vereshchagina LA and Tsokos GC 
In:
J Immunol (2002) 169: 6236-6243 
Categories:
Transfection 
Authors:
Shi GX, Harrison K, Wilson GL, Moratz C and Kehrl JH 
In:
J Immunol (2002) 169(5): 2507-2515 
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