cells

human CML-derived B cell like

Cell Type:

B cell

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	FF-120	2e5	92% ±3	95% ±2	Plasmid (general)	0.4 µg	20 µl	96-well
SF	FF-120	2e5	89-95%	93-97%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	FF-120	1e6	89-95%	93-97%	Plasmid (general)	2 µg	100 µl	4D X-Unit
V	T-016	1e6	89% ±3	88%	Plasmid (general)	1 µg	100 µl	I/II/2b
V	T-003	1e6	79% ±5	89%	Plasmid (general)	1 µg	100 µl	I/II/2b

Citations (88)

Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism

Categories:

Transfection

Authors:

Sébastien Levesque , Andrea Cosentino, Archana Verma , Pietro Genovese , Daniel E Bauer

In:

Nat Biotechnol (2024) :

Variable characteristics overlooked in human K-562 leukemia cell lines with a common signature

Categories:

Cell Lines and Primary Cancer Cells

Authors:

Fumio Kasai *, Kumiko Mizukoshi & Yukio Nakamura

In:

Scientific Reports (2024) 14: 9619

Gene editing of hematopoietic stem cells restores T-cell response in familial hemophagocytic lymphohistiocytosis

Categories:

Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells

Authors:

Dettmer-Monaco V, Weißert K, Ammann S, Monaco G, Lei L, Gräßel L, Rhiel M, Rositzka J, Kaufmann MM, Geiger K, Andrieux G, Lao J, Thoulass G, Schell C, Boerries M, Illert AL, Cornu TI, Ehl S, Aichele P, Cathomen T

In:

J Allergy Clin Immunol (2023) S0091: 6749(23)00989-2

A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells

Categories:

Primary Cells and Media, Transfection

Authors:

Carusillo A, Haider S, Schäfer R, Rhiel M, Türk D, Chmielewski KO, Klermund J, Mosti L, Andrieux G, Schäfer R, Cornu TI, Cathomen T, Mussolino C

In:

Nucleic Acids Res (2023) 51(9): 4660-4673

CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells

Categories:

Primary Cells and Media, Transfection

Authors:

Lin-Shiao E, Pfeifer WG, Shy BR, Saffari Doost M, Chen E, Vykunta VS, Hamilton JR, Stahl EC, Lopez DM, Sandoval Espinoza CR, Deyanov AE, Lew RJ, Poirer MG, Marson A, Castro CE, Doudna JA

In:

Nucleic Acids Res (2022) 50(3): 1256-1268

Efficient C• G-to-G• C base editors developed using CRISPRi screens, target-library analysis, and machine learning

Categories:

Transfection

Authors:

Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, Adamson B, Liu DR.

In:

Nat Biotechnol (2021) 39(11): 1414-1425

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

Categories:

Transfection

Authors:

Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grünewald J, Joung JK

In:

Nat Biotechnol (2021) Jan;39(1): 41-46

CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells

Categories:

Transfection

Authors:

Kluesner MG, Lahr WS, Lonetree CL, Smeester BA, Qiu X, Slipek NJ, Claudio Vázquez PN, Pitzen SP, Pomeroy EJ, Vignes MJ, Lee SC, Bingea SP, Andrew AA, Webber BR, Moriarity BS

In:

Nature (2021) 12(1): 2437

Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair

Categories:

Transfection

Authors:

Schubert MS, Thommandru B, Woodley J, Turk R, Yan S, Kurgan G, McNeill MS, Rettig GR.

In:

Scientific Reports (2021) 11(1): 19482

CRISPR off-target detection with DISCOVER-seq

Categories:

Transfection

Authors:

Wienert B, Wyman SK, Yeh CD, Conklin BR, Corn JE

In:

Nat Protocols (2020) 15(5): 1775-1799

Search-and-replace genome editing without double-strand breaks or donor DNA.

Categories:

Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells

Authors:

Anzalone AV1,2,3, Randolph PB1,2,3, Davis JR1,2,3, Sousa AA1,2,3, Koblan LW1,2,3, Levy JM1,2,3, Chen PJ1,2,3, Wilson C1,2,3, Newby GA1,2,3, Raguram A1,2,3, Liu DR4,5,6.

In:

Nature (2019) 10: 1038

Highly efficient editing of the ß-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell disease

Categories:

Transfection

Authors:

So Hyun Park , Ciaran M Lee , Daniel P Dever , Timothy H Davis , Joab Camarena , Waracharee Srifa , Yankai Zhang , Alireza Paikari , Alicia K Chang , Matthew H Porteus , Vivien A Sheehan , Gang Bao

In:

Nucleic Acids Res (2019) 47(15): 7955-7972

Bacteria-free minicircle DNA system to generate integration-free CAR-T cells

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells

Authors:

Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang

In:

J Med Genetics (2019) 56: 10–17

CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors.

Categories:

Transfection

Authors:

Bak RO, Porteus MH.

In:

Cell Rep (2017) 20(3): 750-756

Marker-free coselection for CRISPR-driven genome editing in human cells.

Categories:

Transfection

Authors:

Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J, Dellaire G, Laganière J, Doyon Y.

In:

Nat Methods (2017) 14(6): 615-620

MiRNA182 regulates percentage of myeloid and erythroid cells in chronic myeloid leukemia

Categories:

Transfection

Authors:

Arya D, Sachithanandan SP, Ross C, Palakodeti D, Li S, Krishna S.

In:

Cell Death Dis. (2017) 2017;8(1): e2547

Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells.

Categories:

Transfection

Authors:

DeWitt MA, Magis W, Bray NL, Wang T, Berman JR, Urbinati F, Heo SJ, Mitros T, Muñoz DP, Boffelli D, Kohn DB, Walters MC, Carroll D, Martin DI, Corn JE.

In:

Science (2016) 8 (360): 1-9

Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing.

Categories:

Transfection

Authors:

Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A.

In:

Cell (2016) 167(1): 219-232

TALENs-mediated gene disruption of FLT3 in leukemia cells: Using genome-editing approach for exploring the molecular basis of gene abnormality.

Categories:

Transfection

Authors:

Wang J, Li T, Zhou M, Hu Z, Zhou X, Zhou S, Wang N, Huang L, Zhao L, Cao Y, Xiao M, Ma D, Zhou P, Shang Z, Zhou J

In:

Scientific Reports (2015) 5:18454: 10.1038

A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells.

Categories:

Authors:

Mathieu Dalvai, Jeremy Loehr, Karine Jacquet, Caroline C. Huard, Céline Roques, Pauline Herst, Jacques Côté , Yannick Doyon

In:

Cell Rep (2015) 13(3): 621-33

Use of Genome Engineering to Create Patient Specific MLL Translocations in Primary Human Hematopoietic Stem and Progenitor Cells.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media, Transfection

Authors:

Breese EH, Buechele C, Dawson C, Cleary ML, Porteus MH.

In:

PLoS ONE (2015) 10(9: e0136644

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

Categories:

Transfection

Authors:

Raj Chari, Prashant Mali, Mark Moosburner, George M Church

In:

Nat Methods (2015) 12(9): 823-6

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

Categories:

Transfection

Authors:

Mandal PK, Ferreira LM, Collins R, Meissner TB, Boutwell CL, Friesen M, Vrbanac V, Garrison BS, Stortchevoi A, Bryder D, Musunuru K, Brand H, Tager AM, Allen TM, Talkowski ME, Rossi DJ, Cowan CA

In:

Cell Stem Cell (2014) 15(5): 643-652

Hydroxyurea-inducible SAR1 gene acts through the Gia/JNK/Jun pathway to regulate ?-globin expression.

Categories:

Primary Cells and Media, Transfection

Authors:

Zhu J, Chin K, Aerbajinai W, Kumkhaek C, Li H, Rodgers GP

In:

Blood (2014) 124(7): 1146-56

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

Categories:

Transfection

Authors:

Kim S, Kim D, Cho SW, Kim J, Kim JS

In:

Genome Res (2014) 24(6): 1012-9

High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs.

Categories:

Transfection

Authors:

Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett EP3, Wandall HH, Davis GD, Hansen SH, Frödin M.

In:

Nucleic Acids Res (2014) 42: 1-16

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

Categories:

Transfection

Authors:

Cho SW, Kim S, Kim Y, Kweon J, Kim HS, Bae S, Kim JS

In:

Genome Res (2014) 24(1): 132–141

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

Categories:

Transfection

Authors:

Yanfang Fu, Jennifer A Foden, Cyd Khayter, Morgan L Maeder, Deepak Reyon, J Keith Joung, Jeffry D Sander

In:

Nat Biotechnol (2013) 31(9): 822-6

RNA-guided human genome engineering via Cas9

Categories:

Transfection

Authors:

Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM

In:

Science (2013) 339(6121): 823-826

Precision genome engineering with programmable DNA-nicking enzymes

Categories:

Transfection

Authors:

Kim E, Kim S, Kim DH, Choi BS, Choi IY, Kim JS

In:

Genome Res (2012) 22(7): 1327(6)

Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme

Categories:

Authors:

Jianbin Wang, Geoffrey Friedman, Yannick Doyon, Nathaniel S. Wang, Carrie Jiaxin Li, Jeffrey C. Miller, Kevin L. Hua, Jenny Jiacheng Yan, Joshua E. Babiarz, Philip D. Gregory, and Michael C. Holmes

In:

Genome Res (2012) 22 (7): 1316-26

The forkhead transcription factor FOXO3a increases phosphoinositide-3 kinase/Akt activity in drug-resistant leukemic cells through induction of PIK3CA expression

Categories:

Transfection

Authors:

Hui RC, Gomes AR, Constantinidou D, Costa JR, Karadedou CT, Fernandez de Mattos S, Wymann MP, Brosens JJ, Schulze A, Lam EW

In:

Mol Cell Biol (2008) 28(19): 5886-98

Mutation in the DC-SIGN cytoplasmic triacidic cluster motif markedly attenuates receptor activity for phagocytosis and endocytosis of mannose-containing ligands by human myeloid cells

Categories:

Transfection

Authors:

Azad AK, Torrelles JB, Schlesinger LS

In:

J Leukoc Biol (2008) epub: online

RNA-loaded CD40-activated B cells stimulate antigen-specific T-cell responses in dogs with spontaneous lymphoma

Categories:

Transfection

Authors:

Mason NJ, Coughlin CM, Overley B, Cohen JN, Mitchell EL, Colligon TA, Clifford CA, Zurbriggen A, Sorenmo KU, Vonderheide RH

In:

Gene Ther (2008) 15(13): 955-65

Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels

Categories:

Transfection

Authors:

Morishita D, Katayama R, Sekimizu K, Tsuruo T, Fujita N

In:

Cancer Res (2008) 68(13): 5076-85

upar induces conformational changes in the integrin alphambeta2 headpiece and re-orientation of its transmembrane domains

Categories:

Transfection

Authors:

Tang ML, Vararattanavech A, Tan SM

In:

J Biol Chem (2008) epub: online

Human AQP5 plays a role in the progression of chronic myelogenous leukemia (CML)

Categories:

Transfection

Authors:

Chae YK, Kang SK, Kim MS, Woo J, Lee J, Chang S, Kim DW, Kim M, Park S, Kim I, Keam B, Rhee J, Koo NH, Park G, Kim SH, Jang SE, Kweon IY, Sidransky D, Moon C

In:

PLoS ONE (2008) 3(7): e2594

Expression of myeloperoxidase enhances the chemosensitivity of leukemia cells through the generation of reactive oxygen species and the nitration of protein

Categories:

Transfection

Authors:

Sawayama Y, Miyazaki Y, Ando K, Horio K, Tsutsumi C, Imanishi D, Tsushima H, Imaizumi Y, Hata T, Fukushima T, Yoshida S, Onimaru Y, Iwanaga M, Taguchi J, Kuriyama K, Tomonaga M

In:

Leukemia (2008) 22(5): 956-64

Subversion of CtBP1-controlled macropinocytosis by human adenovirus serotype 3

Categories:

Transfection

Authors:

Amstutz B, Gastaldelli M, Kälin S, Imelli N, Boucke K, Wandeler E, Mercer J, Hemmi S, Greber UF

In:

EMBO J (2008) 27(7): 956-69

Regulating gene expression in human leukemia cells using light-activated oligodeoxynucleotides

Categories:

Transfection

Authors:

Tang X, Swaminathan J, Gewirtz AM, Dmochowski IJ

In:

Nucleic Acids Res (2008) 36(2): 559-69

Expression of the CTCF-paralogous cancer-testis gene, brother of the regulator of imprinted sites (BORIS), is regulated by three alternative promoters modulated by CpG methylation and by CTCF and p53 transcription factors

Categories:

Transfection

Authors:

Renaud S, Pugacheva EM, Delgado MD, Braunschweig R, Abdullaev Z, Loukinov D, Benhattar J, Lobanenkov V

In:

Nucleic Acids Res (2007) 35(21): 7372-88

Target proteins of C/EBPp30 in AML: C/EBPp30 enhances sumoylation of C/EBPp42 via up-regulation of Ubc9

Categories:

Transfection

Authors:

Geletu M, Balkhi MY, Peer Zada AA, Christopeit M, Anto Pulikkan J, Kumar Trivedi A, Tenen DG, Behre G

In:

Blood (2007) 110(9): 3301-9

Novel RUNX1 isoforms determine the fate of acute myeloid leukemia cells by controlling CD56 expression

Categories:

Transfection

Authors:

Gattenloehner S, Chuvpilo S, Langebrake C, Reinhardt D, Muller-Hermelink HK, Serfling E, Vincent A, Marx A

In:

Blood (2007) 110(6): 2027-33

A novel natural compound, a cycloanthranilylproline derivative (Fuligocandin B), sensitizes leukemia cells to apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) through 15-deoxy-12, 14 prostaglandin J2 production

Categories:

Transfection

Authors:

Hasegawa H, Yamada Y, Komiyama K, Hayashi M, Ishibashi M, Sunazuka T, Izuhara T, Sugahara K, Tsuruda K, Masuda M, Takasu N, Tsukasaki K, Tomonaga M, Kamihira S

In:

Blood (2007) 110(5): 1664-74

Hip is a pro-survival substrate of granzyme B

Categories:

Transfection

Authors:

Hostetter DR, Loeb CR, Chu F, Craik CS

In:

J Biol Chem (2007) 282(38): 27865-74

Mammalian NOTCH-1 activates beta1 integrins via the small GTPase R-Ras

Categories:

Transfection

Authors:

Hodkinson PS, Elliot PA, Lad Y, McHugh BJ, Mackinnon AC, Haslett C, Sethi T

In:

J Biol Chem (2007) 282(39): 28991-9001

Myeloperoxidase Is a Key Regulator of Oxidative Stress Mediated Apoptosis in Myeloid Leukemic Cells

Categories:

Transfection

Authors:

Nakazato T, Sagawa M, Yamato K, Xian M, Yamamoto T, Suematsu M, Ikeda Y, Kizaki M

In:

Clin Cancer Res (2007) 13(18): 5436-5445

Cytokine-Independent Upregulation of MDA5 in Viral Infection

Categories:

Transfection

Authors:

Yount JS, Moran TM, Lopez CB

In:

J Virol (2007) 81(13): 7316-9

An improved zinc-finger nuclease architecture for highly specific genome editing

Categories:

Transfection

Authors:

Miller JC, Holmes MC, Wang J, Guschin DY, Lee YL, Rupniewski I, Beausejour CM, Waite AJ, Wang NS, Kim KA, Gregory PD, Pabo CO, Rebar EJ

In:

Nat Biotechnol (2007) 25(7): 778-85

Synergistic interactions between vorinostat and sorafenib in chronic myelogenous leukemia cells involve Mcl-1 and p21CIP1 down-regulation

Categories:

Transfection

Authors:

Dasmahapatra G, Yerram N, Dai Y, Dent P, Grant S

In:

Clin Cancer Res (2007) 13(14): 4280-90

MicroRNA gene expression during retinoic acid-induced differentiation of human acute promyelocytic leukemia

Categories:

Transfection

Authors:

Garzon R, Pichiorri F, Palumbo T, Visentini M, Aqeilan R, Cimmino A, Wang H, Sun H, Volinia S, Alder H, Calin GA, Liu CG, Andreeff M, Croce CM

In:

Oncogene (2007) 26(28): 4148-57

Proteomic Analysis Identifies Oxidative Stress Induction by Adaphostin

Categories:

Transfection

Authors:

Stockwin LH, Bumke MA, Yu SX, Webb SP, Collins JR, Hollingshead MG, Newton DL

In:

Clin Cancer Res (2007) 13(12): 3667-3681

MEK1/2 inhibitors sensitize BCR/ABL+ human leukemia cells to the dual ABL/SRC inhibitor BMS354825

Categories:

Transfection

Authors:

Nguyen TK, Rahmani M, Harada H, Dent P, Grant S

In:

Blood (2007) 109(9): 4006-15

The chromatin remodeling factor Mi-2alpha acts as a novel co-activator for human c-Myb

Categories:

Transfection

Authors:

Saether T, Berge T, Ledsaak M, Matre V, Alm-Kristiansen AH, Dahle O, Aubry F, Gabrielsen OS

In:

J Biol Chem (2007) 282(19): 13994-4005

Iron deprivation blocks multilineage haematopoietic differentiation by inhibiting induction of p21(WAF1/CIP1).

Categories:

Primary Cells and Media

Authors:

Alcantara O, Boldt DH

In:

Br J of Haematol (2007) 137(3): 252-61

Many NK cell receptors activate ERK2 and JNK1 to trigger microtubule organizing center and granule polarization and cytotoxicity

Categories:

Transfection

Authors:

Chen X, Trivedi PP, Ge B, Krzewski K, Strominger JL

In:

Proc Natl Acad Sci USA (2007) 104(15): 6329-34

c-Myb contributes to G2/M cell cycle transition in human hematopoietic cells by direct regulation of cyclin B1 expression

Categories:

Transfection

Authors:

Nakata Y, Shetzline S, Sakashita C, Kalota A, Rallapalli R, Rudnick SI, Zhang Y, Emerson SG, Gewirtz AM

In:

Mol Cell Biol (2007) 27(6): 2048-58

Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46

Categories:

Transfection

Authors:

Walzer T, Blery M, Chaix J, Fuseri N, Chasson L, Robbins SH, Jaeger S, Andre P, Gauthier L, Daniel L, Chemin K, Morel Y, Dalod M, Imbert J, Pierres M, Moretta A, Romagne F, Vivier E

In:

Proc Natl Acad Sci USA (2007) 104(9): 3384-9

Overexpression of the heat-shock protein 70 is associated to imatinib resistance in chronic myeloid leukemia

Categories:

Transfection

Authors:

Pocaly M, Lagarde V, Etienne G, Ribeil JA, Claverol S, Bonneu M, Moreau-Gaudry F, Guyonnet-Duperat V, Hermine O, Melo JV, Dupouy M, Turcq B, Mahon FX, Pasquet JM

In:

Leukemia (2007) 21(1): 93-101

Histone deacetylase inhibitors deplete enhancer of zeste 2 and associated polycomb repressive complex 2 proteins in human acute leukemia cells

Categories:

Transfection

Authors:

Fiskus W, Pranpat M, Balasis M, Herger B, Rao R, Chinnaiyan A, Atadja P, Bhalla K

In:

Mol Cancer Ther (2006) 5(12): 3096-104

Identification of key genes responsible for cytokine-induced erythroid and myeloid differentiation and switching of hematopoietic stem cells by RAGE

Categories:

Transfection

Authors:

Chen L, Zhang H, Shi Y, Chin KL, Tang DC, Rodgers GP

In:

Cell Res (2006) 16(12): 923-39

A sequential blockade strategy for the design of combination therapies to overcome oncogene addiction in chronic myelogenous leukemia

Categories:

Transfection

Authors:

Chen R, Gandhi V, Plunkett W

In:

Cancer Res (2006) 66(22): 10959-10966

Identification of 12/15-lipoxygenase as a suppressor of myeloproliferative disease

Categories:

Transfection

Authors:

Middleton MK, Zukas AM, Rubinstein T, Jacob M, Zhu P, Zhao L, Blair I, Pure E

In:

J Exp Med (2006) 203(11): 2529-40

Gene therapy for BCR-ABL+ human CML with dual phosphorylation resistant p27Kip1 and stable RNA interference using an EBV vector

Categories:

Transfection

Authors:

Sengupta A, Banerjee D, Chandra S, Banerjee S

In:

J Gene Med (2006) 8(10): 1251-61

Stable gene transfer to human CD34(+) hematopoietic cells using the Sleeping Beauty transposon

Categories:

Transfection

Authors:

Hollis RP, Nightingale SJ, Wang X, Pepper KA, Yu XJ, Barsky L, Crooks GM, Kohn DB

In:

Exp Hematol (2006) 34(10): 1333-43

A novel mechanism for BCR-ABL action: stimulated secretion of CCN3 is involved in growth and differentiation regulation

Categories:

Transfection

Authors:

McCallum L, Price S, Planque N, Perbal B, Pierce A, Whetton AD, Irvine AE

In:

Blood (2006) 108(5): 1716-23

Role of DNA methylation for expression of novel stem cell marker CDCP1 in hematopoietic cells

Categories:

Transfection

Authors:

Kimura H, Morii E, Ikeda JI, Ezoe S, Xu JX, Nakamichi N, Tomita Y, Shibayama H, Kanakura Y, Aozasa K

In:

Leukemia (2006) 20(9): 1551-6

Antagonistic Regulation of -Globin Gene Expression by Helix-Loop-Helix Proteins USF and TFII-I

Categories:

Transfection

Authors:

Crusselle-Davis VJ, Vieira KF, Zhou Z, Anantharaman A, Bungert J

In:

Mol Cell Biol (2006) 26(18): 6832-43

Different roles for the TOS and RAIP motifs of the translational regulator protein 4E-BP1 in the association with raptor and phosphorylation by mTOR in the regulation of cell size

Categories:

Transfection

Authors:

Eguchi S, Tokunaga C, Hidayat S, Oshiro N, Yoshino K, Kikkawa U, Yonezawa K

In:

Genes Cells (2006) 11(7): 757-66

Preclinical Assessment of FHIT Gene Replacement Therapy in Human Leukemia Using a Chimeric Adenovirus, Ad5/F35

Categories:

Transfection

Authors:

Pichiorri F, Trapasso F, Palumbo T, Aqeilan RI, Drusco A, Blaser BW, Iliopoulos D, Caligiuri MA, Huebner K, Croce CM

In:

Clin Cancer Res (2006) 12(11): 3494-501

BCR/ABL Oncogene Directly Controls MHC Class I Chain-Related Molecule A Expression in Chronic Myelogenous Leukemia

Categories:

Transfection

Authors:

Boissel N, Rea D, Tieng V, Dulphy N, Brun M, Cayuela JM, Rousselot P, Tamouza R, Le Bouteiller P, Mahon FX, Steinle A, Charron D, Dombret H, Toubert A

In:

J Immunol (2006) 176(8): 5108-5116

Synergistic Interactions between DMAG and Mitogen-Activated Protein Kinase Kinase 1/2 Inhibitors in Bcr/abl+ Leukemia Cells Sensitive and Resistant to Imatinib Mesylate

Categories:

Transfection

Authors:

Nguyen TK, Rahmani M, Gao N, Kramer L, Corbin AS, Druker BJ, Dent P, Grant S

In:

Clin Cancer Res (2006) 12(7): 2239-2247

Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane

Categories:

Transfection

Authors:

Booth AM, Fang Y, Fallon JK, Yang JM, Hildreth JE, Gould SJ

In:

J Cell Biol (2006) 172(6): 923-35

MicroRNA fingerprints during human megakaryocytopoiesis

Categories:

Transfection

Authors:

Garzon R, Pichiorri F, Palumbo T, Iuliano R, Cimmino A, Aqeilan R, Volinia S, Bhatt D, Alder H, Marcucci G, Calin GA, Liu CG, Bloomfield CD, Andreeff M, Croce CM

In:

Proc Natl Acad Sci USA (2006) 103(13): 5078-83

The hydroxyurea-induced small GTP-binding protein SAR modulates -globin gene expression in human erythroid cells

Categories:

Transfection

Authors:

Tang DC, Zhu J, Liu W, Chin K, Sun J, Chen L, Hanover JA and Rodgers GP

In:

Blood (2005) 94(5): 3278-3291

Rationally targeted, conformationally constrained, oxetane-modified oligonucleotides demonstrate efficient gene-silencing activity in a cellular system

Categories:

Transfection

Authors:

Opalinska JB, Gewirtz AM

In:

Ann NY Acad Sci (2005) 1058: 39-51

Abrogation of heat shock protein 70 induction as a strategy to increase antileukemia activity of heat shock protein 90 inhibitor 17-allylamino-demethoxy geldanamycin

Categories:

Transfection

Authors:

Guo F, Rocha K, Bali P, Pranpat M, Fiskus W, Boyapalle S, Kumaraswamy S, Balasis M, Greedy B, Armitage ES, Lawrence N, Bhalla K

In:

Cancer Res (2005) 65(22): 10536-44

Apoptosis Induced by the Kinase Inhibitor BAY 43-9006 in Human Leukemia Cells Involves Down-regulation of Mcl-1 through Inhibition of Translation

Categories:

Transfection

Authors:

Rahmani M, Maynard Davis E, Bauer C, Dent P and Grant S

In:

J Biol Chem (2005) 280(42): 35217-35227

Synergistic interactions between MEK1/2 and histone deacetylase inhibitors in BCR/ABL+ human leukemia cells

Categories:

Transfection

Authors:

Yu C, Dasmahapatra G, Dent P and Grant S

In:

Leukemia (2005) 19(9): 1579-1589

Inhibition of histone deacetylase 6 acetylates and disrupts the chaperone function of heat shock protein 90: a novel basis for antileukemia activity of histone deacetylase inhibitors

Categories:

Transfection

Authors:

Bali P, Pranpat M, Bradner J, Balasis M, Fiskus W, Guo F, Rocha K, Kumaraswamy S, Boyapalle S, Atadja P, Seto E and Bhalla K

In:

J Biol Chem (2005) 280(29): 26729-26734

Highly efficient endogenous human gene correction using designed zinc-finger nucleases

Categories:

Transfection

Authors:

Urnov FD, Miller JC, Lee YL, Beausejour CM, Rock JM, Augustus S, Jamieson AC, Porteus MH, Gregory PD and Holmes MC

In:

Nature (2005) 435(7042): 646-651

Inhibitory effect of c-Myc on p53-induced apoptosis in leukemia cells. Microarray analysis reveals defective induction of p53 target genes and upregulation of chaperone genes

Categories:

Transfection

Authors:

Ceballos E, Munoz-Alonso MJ, Berwanger B, Acosta JC, Hernandez R, Krause M, Hartmann O, Eilers M and Leon J

In:

Oncogene (2005) 24(28): 4559-4571

Cotreatment with Suberanoylanilide Hydroxamic Acid and 17-Allylamino 17-demethoxygeldanamycin Synergistically Induces Apoptosis in Bcr-Abl+ Cells Sensitive and Resistant to STI571 (Imatinib Mesylate) in Association with Down-Regulation of Bcr-Abl, Abrogation of Signal Transducer and Activator of Transcription 5 Activity, and Bax Conformational Change

Categories:

Transfection

Authors:

Rahmani M, Reese E, Dai Y, Bauer C, Kramer LB, Huang M, Jove R, Dent P and Grant S

In:

Mol Pharmacol (2005) 67(4): 1166-1176

SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc

Categories:

Transfection

Authors:

Tominaga K, Kagata T, Johmura Y, Hishida T, Nishizuka M, Imagawa M

In:

FASEB J (2005) 272(7): 1590-1599

Role of Chk1 and Chk2 in Ara-C-induced differentiation of human leukemia K562 cells

Categories:

Transfection

Authors:

Takagaki K, Katsuma S, Kaminishi Y, Horio T, Tanaka T, Ohgi T and Yano J

In:

Genes Cells (2005) 10(2): 97-106

Short interfering RNA (siRNA) targeting the Lyn kinase induces apoptosis in primary, and drug-resistant, BCR-ABL1(+) leukemia cells

Categories:

Transfection

Authors:

Ptasznik A, Nakata Y, Kalota A, Emerson SG and Gewirtz AM

In:

Nat Med (2004) 10(11): 1187-1189

Neuromedin U: a Myb-regulated autocrine growth factor for human myeloid leukemias

Categories:

Transfection

Authors:

Shetzline SE, Rallapalli R, Dowd KJ, Zou S, Nakata Y, Swider CR, Kalota A, Choi JK and Gewirtz AM

In:

Blood (2004) 104(6): 1833-1840

New non-viral method for gene transfer into primary cells

Categories:

Transfection

Authors:

Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K

In:

Methods (2004) 33(2): 151-163

+ Show All