K-562

human CML-derived B cell like

Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-016 1e6 89% ±3 88% Plasmid (general) 1 µg 100 µl I/II/2b
V T-003 1e6 79% ±5 89% Plasmid (general) 1 µg 100 µl I/II/2b
SF 96-FF-120 2e5 92% ±3 95% ±2 Plasmid (general) 0.4 µg 20 µl Shuttle
SF

FF-120

2e5 89-95% 93-97% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF

FF-120

1e6 89-95% 93-97% Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations (75)

Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang 
In:
J Med Genetics (2019) 56: 10–17 
Categories:
Transfection 
Authors:
Bak RO, Porteus MH. 
In:
Cell Rep (2017) 20(3): 750-756 
Categories:
Transfection 
Authors:
Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J, Dellaire G, Laganière J, Doyon Y. 
In:
Nat Methods (2017) 14(6): 615-620 
Categories:
Transfection 
Authors:
DeWitt MA, Magis W, Bray NL, Wang T, Berman JR, Urbinati F, Heo SJ, Mitros T, Muñoz DP, Boffelli D, Kohn DB, Walters MC, Carroll D, Martin DI, Corn JE. 
In:
Science (2016) 8 (360): 1-9 
Categories:
Transfection 
Authors:
Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. 
In:
Cell (2016) 167(1): 219-232 
Categories:
Transfection 
Authors:
Wang J, Li T, Zhou M, Hu Z, Zhou X, Zhou S, Wang N, Huang L, Zhao L, Cao Y, Xiao M, Ma D, Zhou P, Shang Z, Zhou J 
In:
Scientific Reports (2015) 5:18454: 10.1038 
Categories:
 
Authors:
Mathieu Dalvai, Jeremy Loehr, Karine Jacquet, Caroline C. Huard, Céline Roques, Pauline Herst, Jacques Côté , Yannick Doyon 
In:
Cell Rep (2015) 13(3): 621-33 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Breese EH, Buechele C, Dawson C, Cleary ML, Porteus MH. 
In:
PLoS ONE (2015) 10(9: e0136644 
Categories:
Transfection 
Authors:
Raj Chari, Prashant Mali, Mark Moosburner, George M Church 
In:
Nat Methods (2015) 12(9): 823-6 
Categories:
Transfection 
Authors:
Mandal PK, Ferreira LM, Collins R, Meissner TB, Boutwell CL, Friesen M, Vrbanac V, Garrison BS, Stortchevoi A, Bryder D, Musunuru K, Brand H, Tager AM, Allen TM, Talkowski ME, Rossi DJ, Cowan CA 
In:
Cell Stem Cell (2014) 15(5): 643-652 
Categories:
Primary Cells and Media, Transfection 
Authors:
Zhu J, Chin K, Aerbajinai W, Kumkhaek C, Li H, Rodgers GP 
In:
Blood (2014) 124(7): 1146-56 
Categories:
Transfection 
Authors:
Kim S, Kim D, Cho SW, Kim J, Kim JS 
In:
Genome Res (2014) 24(6): 1012-9 
Categories:
Transfection 
Authors:
Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett EP3, Wandall HH, Davis GD, Hansen SH, Frödin M. 
In:
Nucleic Acids Res (2014) 42: 1-16 
Categories:
Transfection 
Authors:
Cho SW, Kim S, Kim Y, Kweon J, Kim HS, Bae S, Kim JS 
In:
Genome Res (2014) 24(1): 132–141 
Categories:
Transfection 
Authors:
Yanfang Fu, Jennifer A Foden, Cyd Khayter, Morgan L Maeder, Deepak Reyon, J Keith Joung, Jeffry D Sander 
In:
Nat Biotechnol (2013) 31(9): 822-6 
Categories:
Transfection 
Authors:
Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM 
In:
Science (2013) 339(6121): 823-826 
Categories:
Transfection 
Authors:
Kim E, Kim S, Kim DH, Choi BS, Choi IY, Kim JS 
In:
Genome Res (2012) 22(7): 1327(6) 
Categories:
 
Authors:
Jianbin Wang, Geoffrey Friedman, Yannick Doyon, Nathaniel S. Wang, Carrie Jiaxin Li, Jeffrey C. Miller, Kevin L. Hua, Jenny Jiacheng Yan, Joshua E. Babiarz, Philip D. Gregory, and Michael C. Holmes 
In:
Genome Res (2012) 22 (7): 1316-26 
Categories:
Transfection 
Authors:
Hui RC, Gomes AR, Constantinidou D, Costa JR, Karadedou CT, Fernandez de Mattos S, Wymann MP, Brosens JJ, Schulze A, Lam EW 
In:
Mol Cell Biol (2008) 28(19): 5886-98 
Categories:
Transfection 
Authors:
Mason NJ, Coughlin CM, Overley B, Cohen JN, Mitchell EL, Colligon TA, Clifford CA, Zurbriggen A, Sorenmo KU, Vonderheide RH 
In:
Gene Ther (2008) 15(13): 955-65 
Categories:
Transfection 
Authors:
Morishita D, Katayama R, Sekimizu K, Tsuruo T, Fujita N 
In:
Cancer Res (2008) 68(13): 5076-85 
Categories:
Transfection 
Authors:
Tang ML, Vararattanavech A, Tan SM 
In:
J Biol Chem (2008) epub: online 
Categories:
Transfection 
Authors:
Chae YK, Kang SK, Kim MS, Woo J, Lee J, Chang S, Kim DW, Kim M, Park S, Kim I, Keam B, Rhee J, Koo NH, Park G, Kim SH, Jang SE, Kweon IY, Sidransky D, Moon C 
In:
PLoS ONE (2008) 3(7): e2594 
Categories:
Transfection 
Authors:
Sawayama Y, Miyazaki Y, Ando K, Horio K, Tsutsumi C, Imanishi D, Tsushima H, Imaizumi Y, Hata T, Fukushima T, Yoshida S, Onimaru Y, Iwanaga M, Taguchi J, Kuriyama K, Tomonaga M 
In:
Leukemia (2008) 22(5): 956-64 
Categories:
Transfection 
Authors:
Amstutz B, Gastaldelli M, Kälin S, Imelli N, Boucke K, Wandeler E, Mercer J, Hemmi S, Greber UF 
In:
EMBO J (2008) 27(7): 956-69 
Categories:
Transfection 
Authors:
Tang X, Swaminathan J, Gewirtz AM, Dmochowski IJ 
In:
Nucleic Acids Res (2008) 36(2): 559-69 
Categories:
Transfection 
Authors:
Renaud S, Pugacheva EM, Delgado MD, Braunschweig R, Abdullaev Z, Loukinov D, Benhattar J, Lobanenkov V 
In:
Nucleic Acids Res (2007) 35(21): 7372-88 
Categories:
Transfection 
Authors:
Geletu M, Balkhi MY, Peer Zada AA, Christopeit M, Anto Pulikkan J, Kumar Trivedi A, Tenen DG, Behre G 
In:
Blood (2007) 110(9): 3301-9 
Categories:
Transfection 
Authors:
Gattenloehner S, Chuvpilo S, Langebrake C, Reinhardt D, Muller-Hermelink HK, Serfling E, Vincent A, Marx A 
In:
Blood (2007) 110(6): 2027-33 
Categories:
Transfection 
Authors:
Hasegawa H, Yamada Y, Komiyama K, Hayashi M, Ishibashi M, Sunazuka T, Izuhara T, Sugahara K, Tsuruda K, Masuda M, Takasu N, Tsukasaki K, Tomonaga M, Kamihira S 
In:
Blood (2007) 110(5): 1664-74 
Categories:
Transfection 
Authors:
Hostetter DR, Loeb CR, Chu F, Craik CS 
In:
J Biol Chem (2007) 282(38): 27865-74 
Categories:
Transfection 
Authors:
Hodkinson PS, Elliot PA, Lad Y, McHugh BJ, Mackinnon AC, Haslett C, Sethi T 
In:
J Biol Chem (2007) 282(39): 28991-9001 
Categories:
Transfection 
Authors:
Nakazato T, Sagawa M, Yamato K, Xian M, Yamamoto T, Suematsu M, Ikeda Y, Kizaki M 
In:
Clin Cancer Res (2007) 13(18): 5436-5445 
Categories:
Transfection 
Authors:
Yount JS, Moran TM, Lopez CB 
In:
J Virol (2007) 81(13): 7316-9 
Categories:
Transfection 
Authors:
Miller JC, Holmes MC, Wang J, Guschin DY, Lee YL, Rupniewski I, Beausejour CM, Waite AJ, Wang NS, Kim KA, Gregory PD, Pabo CO, Rebar EJ 
In:
Nat Biotechnol (2007) 25(7): 778-85 
Categories:
Transfection 
Authors:
Dasmahapatra G, Yerram N, Dai Y, Dent P, Grant S 
In:
Clin Cancer Res (2007) 13(14): 4280-90 
Categories:
Transfection 
Authors:
Garzon R, Pichiorri F, Palumbo T, Visentini M, Aqeilan R, Cimmino A, Wang H, Sun H, Volinia S, Alder H, Calin GA, Liu CG, Andreeff M, Croce CM 
In:
Oncogene (2007) 26(28): 4148-57 
Categories:
Transfection 
Authors:
Stockwin LH, Bumke MA, Yu SX, Webb SP, Collins JR, Hollingshead MG, Newton DL 
In:
Clin Cancer Res (2007) 13(12): 3667-3681 
Categories:
Transfection 
Authors:
Nguyen TK, Rahmani M, Harada H, Dent P, Grant S 
In:
Blood (2007) 109(9): 4006-15 
Categories:
Transfection 
Authors:
Saether T, Berge T, Ledsaak M, Matre V, Alm-Kristiansen AH, Dahle O, Aubry F, Gabrielsen OS 
In:
J Biol Chem (2007) 282(19): 13994-4005 
Categories:
Primary Cells and Media 
Authors:
Alcantara O, Boldt DH 
In:
Br J of Haematol (2007) 137(3): 252-61 
Categories:
Transfection 
Authors:
Chen X, Trivedi PP, Ge B, Krzewski K, Strominger JL 
In:
Proc Natl Acad Sci USA (2007) 104(15): 6329-34 
Categories:
Transfection 
Authors:
Nakata Y, Shetzline S, Sakashita C, Kalota A, Rallapalli R, Rudnick SI, Zhang Y, Emerson SG, Gewirtz AM 
In:
Mol Cell Biol (2007) 27(6): 2048-58 
Categories:
Transfection 
Authors:
Walzer T, Blery M, Chaix J, Fuseri N, Chasson L, Robbins SH, Jaeger S, Andre P, Gauthier L, Daniel L, Chemin K, Morel Y, Dalod M, Imbert J, Pierres M, Moretta A, Romagne F, Vivier E 
In:
Proc Natl Acad Sci USA (2007) 104(9): 3384-9 
Categories:
Transfection 
Authors:
Pocaly M, Lagarde V, Etienne G, Ribeil JA, Claverol S, Bonneu M, Moreau-Gaudry F, Guyonnet-Duperat V, Hermine O, Melo JV, Dupouy M, Turcq B, Mahon FX, Pasquet JM 
In:
Leukemia (2007) 21(1): 93-101 
Categories:
Transfection 
Authors:
Fiskus W, Pranpat M, Balasis M, Herger B, Rao R, Chinnaiyan A, Atadja P, Bhalla K 
In:
Mol Cancer Ther (2006) 5(12): 3096-104 
Categories:
Transfection 
Authors:
Chen L, Zhang H, Shi Y, Chin KL, Tang DC, Rodgers GP 
In:
Cell Res (2006) 16(12): 923-39 
Categories:
Transfection 
Authors:
Chen R, Gandhi V, Plunkett W 
In:
Cancer Res (2006) 66(22): 10959-10966 
Categories:
Transfection 
Authors:
Middleton MK, Zukas AM, Rubinstein T, Jacob M, Zhu P, Zhao L, Blair I, Pure E 
In:
J Exp Med (2006) 203(11): 2529-40 
Categories:
Transfection 
Authors:
Sengupta A, Banerjee D, Chandra S, Banerjee S 
In:
J Gene Med (2006) 8(10): 1251-61 
Categories:
Transfection 
Authors:
Hollis RP, Nightingale SJ, Wang X, Pepper KA, Yu XJ, Barsky L, Crooks GM, Kohn DB 
In:
Exp Hematol (2006) 34(10): 1333-43 
Categories:
Transfection 
Authors:
McCallum L, Price S, Planque N, Perbal B, Pierce A, Whetton AD, Irvine AE 
In:
Blood (2006) 108(5): 1716-23 
Categories:
Transfection 
Authors:
Kimura H, Morii E, Ikeda JI, Ezoe S, Xu JX, Nakamichi N, Tomita Y, Shibayama H, Kanakura Y, Aozasa K 
In:
Leukemia (2006) 20(9): 1551-6 
Categories:
Transfection 
Authors:
Crusselle-Davis VJ, Vieira KF, Zhou Z, Anantharaman A, Bungert J 
In:
Mol Cell Biol (2006) 26(18): 6832-43 
Categories:
Transfection 
Authors:
Eguchi S, Tokunaga C, Hidayat S, Oshiro N, Yoshino K, Kikkawa U, Yonezawa K 
In:
Genes Cells (2006) 11(7): 757-66 
Categories:
Transfection 
Authors:
Pichiorri F, Trapasso F, Palumbo T, Aqeilan RI, Drusco A, Blaser BW, Iliopoulos D, Caligiuri MA, Huebner K, Croce CM 
In:
Clin Cancer Res (2006) 12(11): 3494-501 
Categories:
Transfection 
Authors:
Boissel N, Rea D, Tieng V, Dulphy N, Brun M, Cayuela JM, Rousselot P, Tamouza R, Le Bouteiller P, Mahon FX, Steinle A, Charron D, Dombret H, Toubert A 
In:
J Immunol (2006) 176(8): 5108-5116 
Categories:
Transfection 
Authors:
Nguyen TK, Rahmani M, Gao N, Kramer L, Corbin AS, Druker BJ, Dent P, Grant S 
In:
Clin Cancer Res (2006) 12(7): 2239-2247 
Categories:
Transfection 
Authors:
Booth AM, Fang Y, Fallon JK, Yang JM, Hildreth JE, Gould SJ 
In:
J Cell Biol (2006) 172(6): 923-35 
Categories:
Transfection 
Authors:
Garzon R, Pichiorri F, Palumbo T, Iuliano R, Cimmino A, Aqeilan R, Volinia S, Bhatt D, Alder H, Marcucci G, Calin GA, Liu CG, Bloomfield CD, Andreeff M, Croce CM 
In:
Proc Natl Acad Sci USA (2006) 103(13): 5078-83 
Categories:
Transfection 
Authors:
Tang DC, Zhu J, Liu W, Chin K, Sun J, Chen L, Hanover JA and Rodgers GP 
In:
Blood (2005) 94(5): 3278-3291 
Categories:
Transfection 
Authors:
Opalinska JB, Gewirtz AM 
In:
Ann NY Acad Sci (2005) 1058: 39-51 
Categories:
Transfection 
Authors:
Guo F, Rocha K, Bali P, Pranpat M, Fiskus W, Boyapalle S, Kumaraswamy S, Balasis M, Greedy B, Armitage ES, Lawrence N, Bhalla K 
In:
Cancer Res (2005) 65(22): 10536-44 
Categories:
Transfection 
Authors:
Rahmani M, Maynard Davis E, Bauer C, Dent P and Grant S 
In:
J Biol Chem (2005) 280(42): 35217-35227 
Categories:
Transfection 
Authors:
Yu C, Dasmahapatra G, Dent P and Grant S 
In:
Leukemia (2005) 19(9): 1579-1589 
Categories:
Transfection 
Authors:
Bali P, Pranpat M, Bradner J, Balasis M, Fiskus W, Guo F, Rocha K, Kumaraswamy S, Boyapalle S, Atadja P, Seto E and Bhalla K 
In:
J Biol Chem (2005) 280(29): 26729-26734 
Categories:
Transfection 
Authors:
Urnov FD, Miller JC, Lee YL, Beausejour CM, Rock JM, Augustus S, Jamieson AC, Porteus MH, Gregory PD and Holmes MC 
In:
Nature (2005) 435(7042): 646-651 
Categories:
Transfection 
Authors:
Ceballos E, Munoz-Alonso MJ, Berwanger B, Acosta JC, Hernandez R, Krause M, Hartmann O, Eilers M and Leon J 
In:
Oncogene (2005) 24(28): 4559-4571 
Categories:
Transfection 
Authors:
Tominaga K, Kagata T, Johmura Y, Hishida T, Nishizuka M, Imagawa M 
In:
FASEB J (2005) 272(7): 1590-1599 
Categories:
Transfection 
Authors:
Takagaki K, Katsuma S, Kaminishi Y, Horio T, Tanaka T, Ohgi T and Yano J 
In:
Genes Cells (2005) 10(2): 97-106 
Categories:
Transfection 
Authors:
Ptasznik A, Nakata Y, Kalota A, Emerson SG and Gewirtz AM 
In:
Nat Med (2004) 10(11): 1187-1189 
Categories:
Transfection 
Authors:
Shetzline SE, Rallapalli R, Dowd KJ, Zou S, Nakata Y, Swider CR, Kalota A, Choi JK and Gewirtz AM 
In:
Blood (2004) 104(6): 1833-1840 
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
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