Data Type
Cell Information
(864)
Citations
(5011)
FAQ
(524)
Culture Media
(61)
Category
Primary Cells and Media
(201)
Transfection
(169)
Laboratory Instrumentation
(73)
Endotoxin and Pyrogen Testing
(63)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
Classical Media and Reagents
(7)
3D Cell Culture
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(136)
Cancer Research/Cell Biology
(78)
Immunotherapy / Hematology
(62)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(41)
Neurobiology
(28)
Cardiovascular
(27)
Respiratory Research
(27)
Molecular Biology
(26)
Toxicology
(23)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(15)
Drug Discovery
(8)
Parasitology
(3)
Gastroenterology
(2)
+ Show All
524 results sorted by
relevance
alphabetical
newest first
oldest first
W
h
a
t
i
s
t
h
e
a
m
o
u
n
t
o
f
D
N
A
I
c
a
n
u
s
e
i
n
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
®
E
x
p
e
r
i
m
e
n
t
?
This totally depends on the Nucleofector® system you are working with. While in the 100 µL reaction vessels usually 1 - 5 µg DNA is being used, for the 20 µL reaction vessels (e.g. X Unit, 96-well Unit and HT...
W
h
i
c
h
p
r
o
m
o
t
e
r
c
a
n
b
e
r
e
c
o
m
m
e
n
d
e
d
f
o
r
t
r
a
n
s
i
e
n
t
g
e
n
e
e
x
p
r
e
s
s
i
o
n
i
n
m
a
m
m
a
l
i
a
n
c
e
l
l
s
?
Vectors with a CMV promoter typically work fine for transient protein expression in mammalian cells, like HEK293, CHO or NSO. For insect cells, like Sf9 or Sf21, vectors with insect promoters are required. In HEK293E (EBNA) cells, the use of...
W
h
e
n
s
h
o
u
l
d
I
b
e
g
i
n
l
o
o
k
i
n
g
f
o
r
t
h
e
L
u
c
i
f
e
r
a
s
e
s
i
g
n
a
l
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
The time course of plasmid expression after Nucleofection® might be different from the time course seen with other transfection methods. We recommend looking at different time points and start with the first time point as early as 4 to 6 hours post...
W
h
a
t
i
s
t
h
e
b
e
s
t
i
n
c
u
b
a
t
i
o
n
t
i
m
e
f
o
r
t
r
a
n
s
i
e
n
t
g
e
n
e
e
x
p
r
e
s
s
i
o
n
i
n
N
u
c
l
e
o
f
e
c
t
i
o
n
®
E
x
p
e
r
i
m
e
n
t
s
?
The optimal incubation time ranges from 2 to 6 days depending on various factors like the stability of the protein in the medium, potential toxicity of the protein, cell density and stability of the plasmid in the nucleus. To check the timepoint for...
W
h
y
i
s
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
T
e
c
h
n
o
l
o
g
y
i
d
e
a
l
f
o
r
t
r
a
n
s
i
e
n
t
p
r
o
t
e
i
n
p
r
o
d
u
c
t
i
o
n
?
For many cell lines in suspension, especially sCHO, conventional transfection methods are very limited in efficiency. For protein production, suspension cells are favored over adherent cells because they are much easier to cultivate. Unlike...
W
h
a
t
s
h
o
u
l
d
I
p
a
y
a
t
t
e
n
t
i
o
n
t
o
w
h
e
n
t
r
a
n
s
f
e
r
r
i
n
g
m
y
r
e
p
o
r
t
e
r
g
e
n
e
s
e
t
u
p
f
r
o
m
a
c
e
l
l
l
i
n
e
t
o
a
p
r
i
m
a
r
y
c
e
l
l
?
In general the overall expression level differs between different cell types. Don't expect the same level of gene induction when working with primary cells. You might need to change to other DNA amounts or ratios.
W
h
y
i
s
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
T
e
c
h
n
o
l
o
g
y
i
d
e
a
l
f
o
r
p
r
o
t
e
i
n
p
r
o
d
u
c
t
i
o
n
f
r
o
m
s
t
a
b
l
e
c
l
o
n
e
s
?
The generation of a stable clone often requires six months or more due to selection procedures and adaption to serum-free conditions after transfection.With the help of the Nucleofector® Technology suspension cells can be transfected directly in...
F
o
r
L
o
n
z
a
'
s
a
n
i
m
a
l
n
e
u
r
o
n
s
,
h
o
w
m
a
n
y
c
o
r
t
i
c
a
l
n
e
u
r
o
n
s
c
a
n
I
e
x
p
e
c
t
f
r
o
m
a
s
i
n
g
l
e
p
u
p
?
According to our manufacturer, you can expect 12 million cortical neurons from an average E18-E19 rat pup.You can expect 4 million cortical neurons from an average mouse pup.
C
a
n
I
a
p
p
l
y
m
o
r
e
t
h
a
n
o
n
e
p
r
o
g
r
a
m
(
t
r
a
n
s
f
e
c
t
i
o
n
c
o
n
d
i
t
i
o
n
)
p
e
r
p
l
a
t
e
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
S
y
s
t
e
m
?
With the 96-well Shuttle® system, it is possible to apply up to 96 different programs (transfection conditions) per 96-well Nucleocuvette® Plate. Therefore, optimization of Nucleofection® Conditions for a particular cell line can be...
W
h
a
t
c
e
l
l
n
u
m
b
e
r
d
o
I
n
e
e
d
p
e
r
w
e
l
l
w
h
e
n
u
s
i
n
g
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
D
e
v
i
c
e
o
r
t
h
e
4
D
-
N
u
c
l
e
o
c
u
v
e
t
t
e
®
S
t
r
i
p
e
s
?
The cell number is very much dependent on the cell type you use. For high throughput Nucleofection®, we usually start with one fifth of the optimal amount recommended for the standard Nucleofection® in the 100µl cuvette. Currently the absolute...
PREVIOUS
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
NEXT