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What microtiter plates would you recommend for running a MycoAlert® and MycoAlert® Plus Assay on a plate luminometer?

We would recommend either Porvair Sciences 204003 or Corning Costar 3912 plates to use with the MycoAlert® and MycoAlert® Plus Assay. Both of these are solid white 96-well plates and give low background and optimal results when used...

What is the difference between the MycoAlert® and MycoAlert® PLUS Assay?

Both assays use the same assay principle, but MycoAlert® PLUS Assay luminesces much brighter than the original MycoAlert® Assay. Because of the higher light output MycoAlert® PLUS Assay may be used on luminometers that were not...
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Is it possible to store reconstituted MycoAlert®/MycoAlert® PLUS Reagent and Substrate?

For optimal assay conditions, reconstituted reagent and substrate should be used fresh within 2 hours after reconstitution.During this time they can be kept at room temperature.For the MycoAlert® Kit: Unused, reconstituted components can...
Missing: plate

Do the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits detect dead mycoplasma?

No. Lonza's MycoAlert® and MycoAlert® PLUS Kits only detect live mycoplasma which gives an accurate assesment of contamination. PCR based detection methods dectect both living and dead mycoplasma, which can lead to false positives. 
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Do antibiotics effect the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits?

Gentamycin and all commonly used antiboitics do not effect MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Assay. We have tested amphotericin, ampicillin, cephalothin, kanamycin, lincomycin, in addition to gentamycin, penicillin and streptomycin...
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Are there positive controls for the MycoAlert® Assay?

The MycoAlert® Assay Control Set (LT07-518) is available separately and includes a lyophilized positive control (1 ml) and assay buffer (2 ml) for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls...
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For hepatocytes, is plating density a concern post Nucleofection®?

Yes, the optimized protocols for the standard Nucleofector® recommend the use of 6 well plates. Plating into a 12-well plate will pose a problem as less cells will attach at high densities.
Missing: mycoalert

Which luminometer is suited for performing the MycoAlert® Assay?

For performing the MycoAlert® Assay in theory every luminometer is suitable as long as a specific sensitivity is given. Please find attached our list with luminometers we know to be suited to run the MycoAlert® and MycoAlert® Plus Mycoplasma...
Missing: plate

Can I use the ViaLight™ Plus Assay also, if my cells are cultured in 24well plates, 6well plates or 12 well plates?

To do the VialIght™ Plus Assay on larger volume tissue culture ( eg 6 well , 24 well) add the cell lysis reagent equal to half the volume in the plate ( ie 100 µl culture use 50 µl cell lysis). Wait 10 minutes for the lysis . Then follow...
Missing: mycoalert

Is the MycoAlert® Assay validated for use in GMP environment?

No. The MycoAlert® Assay is sold for Research Use Only.
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