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What microtiter plates would you recommend for running a MycoAlert® and MycoAlert® Plus Assay on a plate luminometer?

We would recommend either Porvair Sciences 204003 or Corning Costar 3912 plates to use with the MycoAlert® and MycoAlert® Plus Assay. Both of these are solid white 96-well plates and give low background and optimal results when used...

What is the difference between the MycoAlert® and MycoAlert® PLUS Assay?

Both assays use the same assay principle, but MycoAlert® PLUS Assay luminesces much brighter than the original MycoAlert® Assay. Because of the higher light output MycoAlert® PLUS Assay may be used on luminometers that were not...
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Is it possible to store reconstituted MycoAlert®/MycoAlert® PLUS Reagent and Substrate?

For optimal assay conditions, reconstituted reagent and substrate should be used fresh within 2 hours after reconstitution.During this time they can be kept at room temperature.For the MycoAlert® Kit: Unused, reconstituted components can...
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Do the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits detect dead mycoplasma?

No. Lonza's MycoAlert® and MycoAlert® PLUS Kits only detect live mycoplasma which gives an accurate assesment of contamination. PCR based detection methods dectect both living and dead mycoplasma, which can lead to false positives. 
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Do antibiotics effect the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits?

Gentamycin and all commonly used antiboitics do not effect MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Assay. We have tested amphotericin, ampicillin, cephalothin, kanamycin, lincomycin, in addition to gentamycin, penicillin and streptomycin...
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Are there positive controls for the MycoAlert® Assay?

The MycoAlert® Assay Control Set (LT07-518) is available separately and includes a lyophilized positive control (1 ml) and assay buffer (2 ml) for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls...
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Which luminometer is suited for performing the MycoAlert® Assay?

For performing the MycoAlert® Assay in theory every luminometer is suitable as long as a specific sensitivity is given. Please find attached our list with luminometers we know to be suited to run the MycoAlert® and MycoAlert® Plus Mycoplasma...
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Is the MycoAlert® Assay validated for use in GMP environment?

No. The MycoAlert® Assay is sold for Research Use Only.
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What are inhibitors of the MycoAlert® Mycoplasma Detection Kit

The main inhibitors of the MycoAlert® Reaction would be things that affect the luciferase detection. Agents which potentially quench the light signal include high levels of salt, EDTA and other chelating agents, very high levels of sera,...
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Is the classical Lucetta® Luminometer compatible with MycoAlert® PLUS Assay?

Yes. You can use the MycoAlert® mode of the classical Lucetta® Luminometer AAL-1001 for running MycoAlert® PLUS Assay. However, as the MycoAlert® mode was programmed based on standard MycoAlert® Assay and has not be adapted...
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