Search Results

Environmental Toxin Screening Using Human-Derived 3D Bioengineered Liver and Cardiac Organoids

INTRODUCTION:Environmental toxins, such as lead and other heavy metals, pesticides, and other compounds, represent a significant health concern within the USA and around the world. Even in the twenty-first century, a plethora of cities and towns...

Authors: Forsythe SD, Devarasetty M, Shupe T, Bishop C, Atala A, Soker S, Skardal A

SST Peer Reviewed

CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells.

T cells engineered with chimeric antigen receptor (CAR) have been successfully applied to treat advanced refractory B cell malignancy. However, many challenges remain in extending its application toward the treatment of solid tumors. The...

Authors: Zhang Y, Zhang X, Cheng C, Mu W, Liu X, Li N, Wei X, Liu X, Xia C, Wang H.

SST Peer Reviewed

Quantification of focal adhesion dynamics of cell movement based on cell-induced collagen matrix deformation using second-harmonic generation microscopy

Mechanical interactions of living cells with the surrounding environment via focal adhesion (FA) in three dimensions (3-D) play a key role in dynamic biological events, such as tissue regeneration, wound healing, and cancer invasion. Recently,...

Authors: Kang YG1, Jang H2, Yang TD1, Notbohm J3, Choi Y1, Park Y2, Kim BM1

Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells.

Genetic diseases of blood cells are prime candidates for treatment through ex vivo gene editing of CD34+ hematopoietic stem/progenitor cells (HSPCs), and a variety of technologies have been proposed to treat these disorders. Sickle cell disease (SCD)...

Authors: DeWitt MA, Magis W, Bray NL, Wang T, Berman JR, Urbinati F, Heo SJ, Mitros T, Muñoz DP, Boffelli D,...

SST Peer Reviewed

Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR

Insertional mutagenesis is an important risk with all genetically modified cell therapies, including chimeric antigen receptor (CAR)-T cell therapy used for hematological malignancies. Here we describe a new tagmentation-assisted PCR (tag-PCR) system...

Authors: Hamada M1, Nishio N2, Okuno Y3, Suzuki S3, Kawashima N1, Muramatsu H1, Tsubota S4, Wilson MH5,...

SST Peer Reviewed

The Effect of Antidepressants on Mesenchymal Stem Cell Differentiation

Background:Use of antidepressant medications has been linked to detrimental impacts on bone mineral density and osteoporosis; however, the cellular basis behind these observations remains poorly understood. The effect does not appear to be...

Authors: Kruk JS, Bermeo S, Skarratt KK, Fuller SJ, Duque G.

Rhesus iPSC Safe Harbor Gene-Editing Platform for Stable Expression of Transgenes in Differentiated Cells of All Germ Layers.

Nonhuman primate (NHP) induced pluripotent stem cells (iPSCs) offer the opportunity to investigate the safety, feasibility, and efficacy of proposed iPSC-derived cellular delivery in clinically relevant in vivo models. However, there is need for...

Authors: Hong SG, Yada RC, Choi K, Carpentier A, Liang TJ, Merling RK, Sweeney CL, Malech HL, Jung M, Corat...

SST Peer Reviewed

Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis.

BACKGROUND:In this study, we examined the effect of oxidative stress on cellular energy metabolism and pro-angiogenic/pro-inflammatory mechanisms of primary rheumatoid arthritis synovial fibroblast cells (RASFC) and human umbilical vein endothelial...

Authors: Balogh E, Veale DJ, McGarry T, Orr C, Szekanecz Z, Ng CT, Fearon U, Biniecka M

Plocabulin, a novel tubulin-binding agent, inhibits angiogenesis by modulation of microtubule dynamics in endothelial cells

BACKGROUND:Vascular supply of tumors is one of the main targets for cancer therapy. Here, we investigated if plocabulin (PM060184), a novel marine-derived microtubule-binding agent, presents antiangiogenic and vascular-disrupting...

Authors: Galmarini CM1, Martin M2, Bouchet BP2, Guillen-Navarro MJ3, Martínez-Diez M3, Martinez-Leal JF3,...

Preclinical Evaluation of Discorhabdins in Antiangiogenic and Antitumor Models

Elements of the hypoxia inducible factor (HIF) transcriptional system, a key regulator of the cellular hypoxic response, are up-regulated in a range of cancer cells. HIF is fundamentally involved in tumor angiogenesis, invasion, and energy...

Authors: Harris EM1, Strope JD2, Beedie SL3, Huang PA4, Goey AKL5, Cook KM6, Schofield CJ7, Chau CH8, Cadelis...

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Environmental Toxin Screening Using Human-Derived 3D Bioengineered Liver and Cardiac Organoids

CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells.

Quantification of focal adhesion dynamics of cell movement based on cell-induced collagen matrix deformation using second-harmonic generation microscopy

Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells.

Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR

The Effect of Antidepressants on Mesenchymal Stem Cell Differentiation

Rhesus iPSC Safe Harbor Gene-Editing Platform for Stable Expression of Transgenes in Differentiated Cells of All Germ Layers.

Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis.

Plocabulin, a novel tubulin-binding agent, inhibits angiogenesis by modulation of microtubule dynamics in endothelial cells

Preclinical Evaluation of Discorhabdins in Antiangiogenic and Antitumor Models