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Does Lonza offer Cryopreserved Leukopaks?

Lonza CellBio Services is pleased to announce the global availability of cryopreserved leukopaks for research use only applications. The cryopreserved leukopaks are manufactured from peripheral blood of normal human donors at a facility in the United...

Are Lonza's Pro293A and Pro293s media compatible with downstream purification of his-tagged proteins through nickel columns?

Pro293S and Pro293a media are serum-free media that do not contain any inhibitors/chelating agents like EDTA that can interfere in Ni-NTA chromatography. For application in downstream processing, we recommend to dilute the harvest...

Can PyroTec Pro automated systems share the same WinKQCL™ Software database for trending and data security?

Yes, the system is able to leverage the robust enterprise features of the WinKQCL™ Software. This includes storing all information in a WinKQCL™ Software database that can be shared amongst the organization with fine-grain user access controls and...

Can plate dilutions schemes be transferred to WinKQCL™ Software if contained in a LIMS?

Yes, this information can be pulled from a LIMS if available. For example, an export created in CSV can be exported back into LIMS.

What components make up the PyroTec™ PRO robotic solution?

PyroTec™ PRO is comprised of a liquid handling robot developed from the Tecan Freedom EVO® 150 Series with Lonza’s proprietary dynamic scripting for endotoxin testing. The system can be equipped with up to two Sunrise microplate...

Is it possible to automate the PyroTec Pro standard dilutions?

The standard endotoxin dilutions are created by the system based on what is specified in the WinKQCL™ Software Template specific for that assay. The PyroTec™ PRO System can dynamically create the dilution scheme in the WinKQCL™ Software.The system...

What age are the rats used to make the rat aortic smooth muscle cells (R-ASM-580)?

The age of these animals is 6 to 7 weeks.

Are there any consumables that need to be purchased to run PyroTec™ PRO Robotic Solution other than standard reagents, dilution tubes, and microplates?

Yes, specific pipette tips (P/N 0000229884) and troughs (P/N 0000229888) for the robotic system are purchased from Lonza. These consumables are also tested to be free of detectable endotoxin and coa can be provided.

Can you provide more information on the PyroTec Pro validation of endotoxin standards made using pipetting vs. vortexing?

The only manual step required is for the analyst to perform the initial reconstitution and vortex the initial stock CSE (or RSE) solution prior to running the assay. The robot prepares the standard curve. As we do not place a vortex mixer on the...

How much space is required for the PyroTec PRO?

The robot is 145 cm wide, 78 cm deep and 87 cm tall. A minimum of 10 cm around the instrument is required for proper function.Additional 50 cm should be taken into account for the width if the Pyrowave is mounted to the right side of the system to...

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