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Are Lonza's Pro293A and Pro293s media compatible with downstream purification of his-tagged proteins through nickel columns?

Pro293S and Pro293a media are serum-free media that do not contain any inhibitors/chelating agents like EDTA that can interfere in Ni-NTA chromatography. For application in downstream processing, we recommend to dilute the harvest...

What components make up the PyroTec™ PRO robotic solution?

PyroTec™ PRO is comprised of a liquid handling robot developed from the Tecan Freedom EVO® 150 Series with Lonza’s proprietary dynamic scripting for endotoxin testing. The system can be equipped with up to two Sunrise microplate...

Are there any consumables that need to be purchased to run PyroTec™ PRO Robotic Solution other than standard reagents, dilution tubes, and microplates?

Yes, specific pipette tips (P/N 0000229884) and troughs (P/N 0000229888) for the robotic system are purchased from Lonza. These consumables are also tested to be free of detectable endotoxin and coa can be provided.

How much space is required for the PyroTec PRO?

The robot is 145 cm wide, 78 cm deep and 87 cm tall. A minimum of 10 cm around the instrument is required for proper function.Additional 50 cm should be taken into account for the width if the Pyrowave is mounted to the right side of the system to...

What are the PyroTec Pro power requirements?

100-240 Volts50/60 MHz1,200 VA

Do you offer on-site maintencance services for the 4D-Nucleofector® Core, X, Y, 96well or LV Unit?

No, currently we can only offer in-house maintenance services, but we are working on a process for on-site services.

Which type of liquid handling arm does the PyroTec™ PRO use, liquid or air driven?

The air LiHa ™ forms a core element of Lonza’s PyroTec™ PRO automated solution for endotoxin testing. The air LiHa™ uses air displacement technology to offer increased flexibility and greater convenience for a variety of applications requiring...

Can you provide more information on the PyroTec Pro validation of endotoxin standards made using pipetting vs. vortexing?

The only manual step required is for the analyst to perform the initial reconstitution and vortex the initial stock CSE (or RSE) solution prior to running the assay. The robot prepares the standard curve. As we do not place a vortex mixer on the...

Can the PyroTec PRO System interface with LIMS?

The automated endotoxin detection system is operated by our WinKQCL software. In this software the end-user can setup and define the trigger for the data transfer. Lonza offers services to assist with this.

How does PyroTec Pro liquid level detection work if different sample containers are used (conductive or pressure based)?

Conductive liquid detection is used by the PyroTec PRO™ System. The system currently supports sample handling in 13 x 100 mm tubes. An option to allow use of additional 5 ml tubes with caps is planned for a future release.

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