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How to prepare a BulletKit Medium?

The SingleQuots™ Kit provided in the BulletKit™ Media have been premeasured for the bottle of basal medium also provided in the kit. For most BulletKit™ Media, simply thaw the SingleQuots™ and add them to the bottle of basal medium. The complete...

How does Lonza perform the cell count on the fresh, unprocessed bone marrow? Does Lonza guarantee a minimum viability for the fresh, unprocessed bone marrow?

Lonza use a Coulter Counter to perform the total cell count on the fresh, unprocessed bone marrow. Lonza does not perform viability testing on this product, but when shipped overnight, viability is typically around 80%.

Are Lonza Bacterial Endotoxin Detection (BET) reagents FDA approved?

All Lonza LAL based products, as well as the facility they are produced in, are FDA licensed.

How do I cryopreserve Lonzas primary cells? What media cocktail are Lonzas primary cells cryopreserved in?

Please be aware that cryopreservation may compromise cell quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™ Cells that have been cryopreserved outside of Lonza. To avoid loss of cells and forfeiture of your...

What concentration of Trypsin/EDTA do you recommend for subculturing primary cells?

For most cell types, Lonza recommends the Trypsin/EDTA provided as one of the components in our ReagentPack™ Subculturing Reagents Kit (CC-5034).The concentration of this solution is proprietary.

Are the horseshoe crabs killed or injured during the harvest process?

Lonza takes great care to not harm the crabs during the harvesting of their blood. During harvesting blood is collected by puncturing the septum on the underside of the animal with a small needle. The animal will only give as much blood as it can...

How long does it take for the fresh bone marrow sample to arrive within the United states from the moment of harvest?

Barring any delays at airport customs, and assuming there are no weather related delays, the sample can be delivered anywhere in the continental United States within 26 hours of collection (same day delivery may be available for local deliveries,...

Does Lonza have products for oven validation depyrogenation studies?

Lonza offers Endotoxin Challenge Vials with part code 00192568 and High Potency Endotoxin with part code 00193763 for oven validation.

When preforming Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene recombinant Factor C (rFC), endotoxin detection kit to establish the potency of Endotoxin Challenge Vials (ECV) can I use a syringe to reconstitute the ECV and other assay reagents?

We do not recommend that a needle and syringe be used to reconstitute ECV or other LAL reagents. The plungers of plastic syringes are generally coated with a lubricant and tend to interfere with the assay. We recommend the tops be removed and diluent...

Where are Lonzas Human Skeletal Muscle Myoblasts (HSMM) cells isolated from?

The Human Skeletal Muscle Myoblasts are sourced from normal post-gestational tissue of cadavers from 1 day to 40 years old. The HSMMs are isolated from the quadriceps or psoas muscle.
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