Q:

Is Lonza's Pro293-A media compatible with downstream purification of his-tagged proteins through nickel columns?

A:

Pro293S media is serum-free medium that does not contain any inhibitors/chelating agents like EDTA that can interfere in Ni-NTA chromatography. For application in downstream processing, we recommend to dilute the harvest to 5 to 10-fold using equilibration buffer and adjust the pH 8.0 before binding.

Related Cells:
Categories:
Serum-free and Speciality Media
Research Areas:
Uncategorized