GP2-293

GP2-293 cell line stably expresses the viral gag and pol proteins. HEK-293 derivative.

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
human
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V A-020 30-40% 100% Plasmid (general) 2 µg 100 µl I/II/2b
V X-001 80-90% 40-50% Plasmid (general) 2 µg 100 µl I/II/2b
V X-005 80-90% 40-50% Plasmid (general) 2 µg 100 µl I/II/2b
V L-029 70-80% 40-50% Plasmid (general) 2 µg 100 µl I/II/2b