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No. Serum will induce squamous differentiation of primary airway epithelial cells. Our SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit CC-3118 does not contain FBS or any other serum.
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There are usually ciliated cells present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process. However, the cilia do not persist during submers culture and are...
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HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH...
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EDTA is a chelator of metal ions like calcium and magensium. It is used with trypsin because it helps bind up the divalent cations in the medium. It helps to keep the cells from sticking to each other. If its not there….you would need a higher...
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The lysis of the cells with the Cell Lysis Reagent included in the ViaLight™ Plus Kit should not affect the DNA. Whilst we haven't conducted this experiment ourselves we cannot see any real reason why it should not be possible to...
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There should be no problem with the use of the ViaLight™ Plus Kit with immobilized antibodies. You can if you wish confirm this with your own experiment by conducting a control experiment as follows:Coat triplicate wells of a 96 well plate with each...
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The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors).Transfection protocols can be found...
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Yes. Please see our Techincal Reference Guide which contains many helpful tips.You can find additional information in the Optimized Nucleofection Protocol for your cell type of interest.
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Yes, by taking a sample of the culture media (usually 20µl) into a fresh luminometer plate this can be assayed with the Adenylate Kinase Detection Reagent supplied in the Toxilight kit to look for increased levels of AK over the control. The rest of...
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Yes. Just by taking a sample of the culture medium (usually 20µl) into a fresh luminometer plate and assaying it using the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit you can see if increased levels of Adenylate...
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