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How many 100 µl Nucleocuvettes® can be transfected in parallel using the 4D-Nucleofector® System?

The 4D-Nucleofector® X Unit can work with two 100µl Nucleocuvettes® in parallel. However, the operation software allows defining up to 50 Nucleocuvettes® per experiment. These cuvettes will be transfected by the system continuously in...

Does the recommended media for each of the diabetic cells contain glucose?

The recommended media does contain glucose. Please contact Scientific Support for glucose concentrations.

What is a GTG™ Agarose?

GTG™ stands for Genetic Technology Grade™. GTG™ Grade Agarose is recommended for preparative DNA electrophoresis, or when further enzymatic manipulation of DNA is required. These agaroses are extensively tested to ensure maximum compatibility with...

Can I optimize the transfection efficiency or cell viability after the 4D-Nucleofector® Cell Line Optimization any further?

We offer a program fine tuning matrix for Optimization of Nucleofection® Conditions - A Short Guideline (lonza.com)Based on your current most satisfactory pulse(s) it provides guidelines which further programs to test to either increase cell...

Do I need to purchase a special chamber to use Reliant™ and Latitude™ Precast Gels?

Reliant™ and Latitude™ Precast Agarose Gels are designed to run in standard horizontal electrophoresis chambers.As long as there is room on the chamber platform for the gel, the chamber should be suitable. Measure the chamber platform and check...

Is the viability of cells from diseased or diabetic donors less than cells from normal donors?

We guarantee > 70 % viability for cells from both diseased/diabetic donors and normal donors.

Are the materials in the FlashGel™ Cassette hazardous?

The stain in the FlashGel™ Cassette is present at such low levels that it is not considered hazardous according to OSHA and EU hazard criteria. A copy of the MSDS is available online. However, as the stain in the cassette is a potential mutagen, wear...

Do we have any Diseased Human Bronchial/Tracheal Epithelial Cells (DHBE) screened for Air Liquid Interface (ALI) differentiation using the B-ALI™ BulletKit™?

Yes. All our DHBEs are also offered in a version screened for ALI differentiation. The lots which do pass this additional ALI testing are electronically designated by adding an “S” to the end of the original catalog number.

How many passages can I use the Clonetics™ diseased cells?

Proliferative capacity of Lonza's Clonetics™ diseased cells is tested through one or more passages (depending on the cell type) for information only.It is NOT a specification where a minimum number of doublings must be obtained in order to pass...

I stained my gel with SYBR Green and recovered my band of interest. However, my DNA is still green. Can I remove the SYBR stain?

Yes. The SYBR stains can easily be removed from nucleic acids by ethanol precipitation. Isopropyl alcohol precipitation is somewhat less effective at removing the dye. Butyl alcohol extraction, chloroform extraction and phenol extraction do not...

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