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523 results sorted by
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Yes. Please click on the link below
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There should be no problem with the use of the ViaLight™ Plus Kit with immobilized antibodies. You can if you wish confirm this with your own experiment by conducting a control experiment as follows:Coat triplicate wells of a 96 well plate with each...
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The TRAP kit Lonza used in the past when testing Osteoclast differentiation/functionality is the Acid Phosphatase, Leukocyte (TRAP) Kit from Sigma-Aldrich (Sigma-Aldrich catalog no. 386A). Now, we are using our OsteoLyse Assay Kit PA-1500 for testing...
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The protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for plating cells into a 96-well plate.
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Yes, by taking a sample of the culture media (usually 20µl) into a fresh luminometer plate this can be assayed with the Adenylate Kinase Detection Reagent supplied in the Toxilight kit to look for increased levels of AK over the control. The rest of...
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HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH...
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EDTA is a chelator of metal ions like calcium and magensium. It is used with trypsin because it helps bind up the divalent cations in the medium. It helps to keep the cells from sticking to each other. If its not there….you would need a higher...
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?
Yes. Just by taking a sample of the culture medium (usually 20µl) into a fresh luminometer plate and assaying it using the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit you can see if increased levels of Adenylate...
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The LGM-3™ (catalog no. CC-3211) media contains gentamicin sulfate at a final concentration of 50 mg/L. LGM-3™ also does contain phenol red. Lonza does not currently offer a phenol red or antibiotic free version or alternative as a...
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Proliferation shows increased Vialight™ Plus RLUs (Relative Light Units) but NO increase in ToxiLight™ RLUs*Cytotoxicity shows decreased Vialight™ Plus RLUs and increased ToxiLight™ RLUs*Cytostasis (where the cells are alive but unable to...
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