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What is the recommended media for culturing Lonza's bone marrow stromal cells?

The recommended media for culturing Lonza's bone marrow stromal cells is MyeloCult™ H5100 from StemCell Technologies, Inc.

What cells make up the population of cells in Lonza's bone marrow stromal cells?

The stromal layer is composed of many cell types including fibroblasts, mesenchymal stem cells, adipocytes, endothelial cells, and macrophages.

What is the recommended culturing protocol for Lonza's bone marrow stromal cells (2M-302)?

Lonza's bone marrow stromal cells should be thawed according to Lonza's "Procedure for thawing Poietics™ Cells". Upon thaw, cells should be plated at 3,000-4,000 cells per cm² in MyeloCult™ H5100 from StemCell Technologies, Inc. An adherent...

What is the component labeled "MCGS" in the mesenchymal stem cell growth and differentiation media?

Mesenchymal Cell Growth Supplement (MCGS) is 100% fetal bovine serum prescreened to neither induce nor prevent differentiation of human bone marrow derived stem cells PT-2501

Do mesenchymal stem cells cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) require an extracellular matrix/plate coating?

While, cells may be able to be cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) without an extracellular matrix/plate coating, for peak performance, a 5ug/mL fibronectin coating should be used. Alternative plate...

Does the basal media portion of the TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) contain proteins?

The basal media portion of the TheraPEAK™ Mesenchymal Stem Cell Growth Medium (MSCGM™), catalog no BEBP12-934Q, contains recombinant and human serum derived proteins.

What testing methods are currently used to validate differentiation of Lonza's mesenchymal stem cells?

Lonza currently validates trilineage differentiation of human mesenchymal stem cells using the following quantitative assays:Adipogenic Differentiation: lipid vacuole binding with AdipoRed (catalog no. PT-7009)Osteogenic Differentiation: inorganic...

Where are Lonza's human Mesenchymal Stem Cells isolated from?

Lonza's human Mesenchymal Stem Cells (hMSCs) PT-2501 are isolated from bone marrow withdrawn from bilateral punctures of the posterior iliac crests of normal volunteers between the ages of 18 and 45 years old. Please see the linked document for more...

With regards to mesenchymal stem cells, what are "Dexter cultures"?

Dexter culture refers to the method of isolating stem cells proposed by Dexter et al in their 1977 paper: Dexter TM, Allen TD, Lajtha LG. Conditions controlling the proliferation of haemopoietic stem cells in vitro. JCell Physiol 1977; 91: 335–344....

Where are Lonza’s dermal cells isolated from?

Lonza is offering different dermal cell types, which are either isolated from adult or from neonatal tissue.Lonza’s adult dermal cells (including keratinocytes, microvascular endothelial cells, fibroblasts, and melanocytes) are usually isolated from...

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