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Where are Lonza's Bronchial Smooth Muscle Cells isolated from?

Lonza’s Bronchial Smooth Muscle Cells (both normal and diseased) are isolated from the bronchial tubes. The region used is around the bifurcation of the bronchial tubes to the lungs and small sections of the bronchia and trachea just off of the...

Is the classical Lucetta® Luminometer compatible with MycoAlert® PLUS Assay?

Yes. You can use the MycoAlert® mode of the classical Lucetta® Luminometer AAL-1001 for running MycoAlert® PLUS Assay. However, as the MycoAlert® mode was programmed based on standard MycoAlert® Assay and has not be adapted...

What is the maximum well volume for the FlashGel Cassettes?

The maximum volume per well for the standard DNA and RNA cassettes is 5 µl. The recovery cassettes are designed to hold more and can be filled to 12 µl.

What happens if I load too much or too little sample on the FlashGel® Cassette?

The FlashGel® System is very flexible. It is best to experiment with the system and adjust the levels to optimize your results.Optimal DNA load levels are 5-20 ng per band in a 5 µl load.DNA fragments = 5 ng are visible on the...

Where are Lonza's normal Adipose Derived Stem Cells isolated from?

Lonza's normal (non-diabetic) Adipose Derived Stem Cells (ADSCs) are isolated from lipoaspirate obtained from liposuction surgeries. As liposuction surgeries typically pool lipoaspirate from multiple locations, a single isolation source is not able...

At what voltage do I need to run the FlashGel® Cassettes? Can I speed up or slow down the run?

We recommend that the FlashGel® DNA cassettes be run at 275 V and RNA cassettes at 225 V. We do not recommend running a gel at a higher voltage than 275 V.Runs at lower voltage for longer time periods may improve separation of larger fragments...

What is the FlashGel Mask?

The FlashGel™ Mask (Cat. No. 57020) is a flexible strip that slides under the FlashGel™ Cassette to block light from the second tier of wells when using double-tier cassettes.

Why do I need to store cell culture media in the dark?

Most tissue culturists will never observe effects on proliferating cells that can be attributed to light exposed media. However, several light-related factors may exert sufficient collective influence to cause the spurious results occasionally...

Which Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection kit should I use?

There are a few different things you should consider before deciding which endotoxin detection kit to order. First of all, how many samples will you be testing, and how frequently? If you are going to be testing one or two samples, one time only,...
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