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From the bottom of one well to the top of the next, the distance is 31mm.
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No. The DNA or RNA will need to be extracted and purified before the samples can be loaded and run on the FlashGel™ Cassettes.
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Lonza's mononuclear cells are isolated from bone marrow, cord blood, or peripheral blood via density gradient centrifugation
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Mononuclear cells are any blood cells with a rounded nucleus including lymphocytes (T-cells, B-cells, and Natural Killer [NK] Cells), monocytes, and dendritic cells.
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Lonza's CD14+ monocytes are isolated first by obtaining mononuclear cells from peripheral blood via density gradient centrifugation. The CD14+ cells are then isolated form the mononuclear cell population using either positive or negative...
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The recommended media for culturing Lonza's bone marrow stromal cells is MyeloCult™ H5100 from StemCell Technologies, Inc.
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Optimal assay working temperature for all components is 18°C-22°C (for MycoAlert® Assay) and 20-22°C (for MycoAlert® PLUS Assay). In case room temperature significantly deviates from this optimal temperature range, it is recommended to use...
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The stromal layer is composed of many cell types including fibroblasts, mesenchymal stem cells, adipocytes, endothelial cells, and macrophages.
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Lonza's bone marrow stromal cells should be thawed according to Lonza's "Procedure for thawing Poietics™ Cells". Upon thaw, cells should be plated at 3,000-4,000 cells per cm² in MyeloCult™ H5100 from StemCell Technologies, Inc. An adherent...
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The TRAP kit Lonza used in the past when testing Osteoclast differentiation/functionality is the Acid Phosphatase, Leukocyte (TRAP) Kit from Sigma-Aldrich (Sigma-Aldrich catalog no. 386A). Now, we are using our OsteoLyse Assay Kit PA-1500 for testing...
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