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What is the stain in the FlashGel® Cassette?

The stain in the FlashGel® Cassettes is a proprietary fluorescent nucleic acid stain. It is not commercially available as a separate stain.

Does the FlashGel System have a built-in power supply?

The dock includes a low-voltage power supply for the transilluminator. The system requires an external electrophoresis power supply with up to a 275 V capacity. A FlashGel™ Power Supply is sold separately (cat. No. 57068) or as part of the FlashGel™...

Does the FlashGel System have a built-in transilluminator?

The FlashGel™ Dock has a built-in blue light transilluminator for real time visualization of the bands.

What wavelength will excite the stain in the FlashGel Cassettes? Can I use a UV transilluminator?

The FlashGel™ Dock has a blue light transilluminator that excites the fluorescent dye at 450 nm. The stain has a secondary excitation peak in the UV range (i.e., 302 nm and 312 nm). Therefore, the bands can also be detected on a UV transilluminator.

What is the maximum well volume for the FlashGel Cassettes?

The maximum volume per well for the standard DNA and RNA cassettes is 5 µl. The recovery cassettes are designed to hold more and can be filled to 12 µl.

What happens if I load too much or too little sample on the FlashGel® Cassette?

The FlashGel® System is very flexible. It is best to experiment with the system and adjust the levels to optimize your results.Optimal DNA load levels are 5-20 ng per band in a 5 µl load.DNA fragments = 5 ng are visible on the...

At what voltage do I need to run the FlashGel® Cassettes? Can I speed up or slow down the run?

We recommend that the FlashGel® DNA cassettes be run at 275 V and RNA cassettes at 225 V. We do not recommend running a gel at a higher voltage than 275 V.Runs at lower voltage for longer time periods may improve separation of larger fragments...

What is the FlashGel Mask?

The FlashGel™ Mask (Cat. No. 57020) is a flexible strip that slides under the FlashGel™ Cassette to block light from the second tier of wells when using double-tier cassettes.

Why do I need to store cell culture media in the dark?

Most tissue culturists will never observe effects on proliferating cells that can be attributed to light exposed media. However, several light-related factors may exert sufficient collective influence to cause the spurious results occasionally...

Which Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection kit should I use?

There are a few different things you should consider before deciding which endotoxin detection kit to order. First of all, how many samples will you be testing, and how frequently? If you are going to be testing one or two samples, one time only,...
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