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The dock includes a low-voltage power supply for the transilluminator. The system requires an external electrophoresis power supply with up to a 275 V capacity. A FlashGel™ Power Supply is sold separately (cat. No. 57068) or as part of the FlashGel™...
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The FlashGel™ Dock has a built-in blue light transilluminator for real time visualization of the bands.
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The FlashGel™ Dock has a blue light transilluminator that excites the fluorescent dye at 450 nm. The stain has a secondary excitation peak in the UV range (i.e., 302 nm and 312 nm). Therefore, the bands can also be detected on a UV transilluminator.
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The maximum volume per well for the standard DNA and RNA cassettes is 5 µl. The recovery cassettes are designed to hold more and can be filled to 12 µl.
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Optimal assay working temperature for all components is 18°C-22°C (for MycoAlert® Assay) and 20-22°C (for MycoAlert® PLUS Assay). In case room temperature significantly deviates from this optimal temperature range, it is recommended to use...
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The FlashGel® System is very flexible. It is best to experiment with the system and adjust the levels to optimize your results.Optimal DNA load levels are 5-20 ng per band in a 5 µl load.DNA fragments = 5 ng are visible on the...
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Both assays use the same assay principle, but MycoAlert® PLUS Assay luminesces much brighter than the original MycoAlert® Assay. Because of the higher light output MycoAlert® PLUS Assay may be used on luminometers that were not...
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We recommend that the FlashGel® DNA cassettes be run at 275 V and RNA cassettes at 225 V. We do not recommend running a gel at a higher voltage than 275 V.Runs at lower voltage for longer time periods may improve separation of larger fragments...
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The FlashGel™ Mask (Cat. No. 57020) is a flexible strip that slides under the FlashGel™ Cassette to block light from the second tier of wells when using double-tier cassettes.
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Most tissue culturists will never observe effects on proliferating cells that can be attributed to light exposed media. However, several light-related factors may exert sufficient collective influence to cause the spurious results occasionally...
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