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When performing the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene recombinant Factor C (rFC), endotoxin detection kit how do I know my pipettes are working adequately?

Check your pipetting accuracy. This is important in every phase of LAL testing. Make sure you are adding the correct amounts of material. Good quality graduated pipettes are already calibrated by the manufacturer, but it is up to the user to use them...

What is the stain in the FlashGel® Cassette?

The stain in the FlashGel® Cassettes is a proprietary fluorescent nucleic acid stain. It is not commercially available as a separate stain.

When performing the Lonza Bacterial Endotoxin Test (BET), LAL gel clot endotoxin detection kit how do I know if I have a positive result for gel clot?

A positive result is a firm gel which maintains its integrity through inversion to 180°. Resist the temptation to look at any of the test tubes before the hour is up. When reading your test results after the one-hour incubation gently withdraw each...

What is the general anatomy of the human prostate? From which portion of the human prostate are Lonza's prostate cells are derived?

The human prostate can generally be described as being glandular epithelium (parenchymal) surrounded by a fibromuscular stroma.The parenchymal portion of the prostate, or of any organ in the body, is epithelial like in nature and contains the “worker...

Does the FlashGel System have a built-in power supply?

The dock includes a low-voltage power supply for the transilluminator. The system requires an external electrophoresis power supply with up to a 275 V capacity. A FlashGel™ Power Supply is sold separately (cat. No. 57068) or as part of the FlashGel™...

Does the FlashGel System have a built-in transilluminator?

The FlashGel™ Dock has a built-in blue light transilluminator for real time visualization of the bands.

Can Lonzas PrEC cells be co-cultured with Lonzas PrSC cells?

While Lonza has never attempted to co-culture Prostate Epithelial Cells (PrEC) and Prostate Stromal Cells (PrSC) together in-house, the recommendation of Lonza Research & Development would be to plate and culture the PrEC and PrSC cells together...

Where are Lonzas PrSC cells isolated from? What cells will be in the PrSC population?

Lonza’s Prostate Stromal Cells (PrSC) are isolated from the stroma of the prostate. Lonza’s Prostate Stromal Cells (PrSC) are a mixed population of cells, however, the majority of the cells in this population are fibroblastic in origin. It is not...

What wavelength will excite the stain in the FlashGel Cassettes? Can I use a UV transilluminator?

The FlashGel™ Dock has a blue light transilluminator that excites the fluorescent dye at 450 nm. The stain has a secondary excitation peak in the UV range (i.e., 302 nm and 312 nm). Therefore, the bands can also be detected on a UV transilluminator.

How should I prepare and handle my product samples for Bacterial Endotoxin Testing (BET) when using Lonza endotoxin detection assays? How do I adjust the pH?

As with handling of the lysate test reagents, the key word is cleanliness. Use aseptic and apyrogenic technique. Check the product for inhibition. Some products, especially members of the small volume parenteral family, may contain substances at...
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