Search Results

Can I also use the Lucetta® 2 Luminometer for other assays besides the MycoAlert® Mycoplasma Detection Assay?

Yes. In addition to the tailor-made MycoAlert® Mode it also has a "Single Read" mode which is suited for unprocessed luminescence readings, e.g. ATP-based cell proliferation or cytotoxicity assays, or Luciferase reporter gene assays.

How much sample do you need for accurate results with MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits? Do you need cells or just supernatant?

The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.

Are there positive controls for the MycoAlert® Assay?

...are designed for use with the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit for the detection of mycoplasma contaminations in cell cultures. The MycoAlert® Assay Control Set may be used to ensure that the operator and all...

Why can’t I obtain a Certificate of Analysis (COA) for a MycoAlert®/MycoAlert® PLUS/ToxiLight®/ViaLight® Kit via your Webpage-tool?

Lonza’s luminescent assays (including MycoAlert® Mycoplasma detection assay, MycoAlert® PLUS Mycoplasma detection assay, MycoAlert® Control set, ToxiLight® Non-Destructive Cytotoxicity Assay, and ViaLight® Plus Cell Proliferation...

Do bacteria lead to false positive MycoAlert® Results?

...is characterized by rapid appearance of turbidity in cell culture media. One of the dangers of mycoplasma infection is that they do not produce turbidity, hence the need for a test such as the MycoAlert® Assay....

Which luminometer is suited for performing the MycoAlert® Assay?

For performing the MycoAlert® Assay in theory every luminometer is suitable as long as a specific sensitivity is given. Please find attached our list with luminometers we know to be suited to run the MycoAlert® and MycoAlert® Plus Mycoplasma...

Why do we recommend that MycoZap™ Reagent 1 treatment must be conducted in media containing a maximum of 5% (v/v) serum? What happens if by mistake a higher serum percentage was used?

As serum is complexing the MycoZap™ Reagent 1 you would decrease the effective concentration and thus inhibit the elimination reaction. If by mistake the elimination was started with higher serum concentration, no elimination took place. The process...

Data Type

Category

Research Area