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Why is the morphology of Lonza’s lung microvascular endothelial cells (HMVEC-L) CC-2527 different from most other endothelial cells?

While most endothelial cells will have a “paving stone” type morphology, Lonza’s lung microvascular endothelial cells (HMVEC-L) will generally appear more stretched and elongated, especially at low confluence. This is a normal morphological...

When running a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay why should I use glass accesory products instead of plastic?

Endotoxin adheres to plastic surfaces more strongly than to glass surfaces. Therefore, we recommend that you use only borosilicate glass dilution tubes when preparing your Control Standard Endotoxin (CSE) dilutions. In some cases, certain plastics...

Where are Lonza’s Normal Human Neural Progenitor (NHNP) and Normal Human Astrocytes (NHA) cells isolated from?

Lonza’s Normal Human Neural Progenitor (NHNP) and Normal Human Astrocytes (NHA) cells are primarily isolated from the cerebral cortex (or forebrain from cerebral hemisphere) from fragmented fetal brain tissue (16-20 weeks old).

When running a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection kit why is it important to vortex my control standard endotoxin (CSE) dilutions?

Endotoxin will adhere to glass surfaces, but this can be counteracted with proper vortexing to ensure that the solution you aliquot into your reaction tubes or microplate has the proper EU/ml concentration. As our package inserts state, the CSE vial...

How old are the donors for Lonza’s dermal cells from neonatal donors?

The neonatal foreskin tissue used to isolate Lonza’s dermal cells from neonatal donors is almost always collected within the first few days after birth, and at most a week or two after birth. At most U.S. hospitals, circumcisions are performed before...

How do I promote keratinocyte terminal differentiation towards corneocytes?

Keratinocyte differentiation can be induced by increasing the concentration of calcium and/or by adding serum to the media. Cells should be initially plated using the standard culture media and conditions and allowed ample time to recover from...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay how do I convert from EU/ml (Endotoxin Unit) to ng/ml?

Depending on the source of the endotoxin, the conversion from endotoxin units to nanograms will vary. The FDA initially defined the Endotoxin Unit (EU) as the endotoxin activity of 0.2 ng of Reference Endotoxin Standard, EC-2 or 5 EU/ng. To convert...

How do I prevent keratinocyte terminal differentiation towards corneocytes? Can Lonza’s keratinocytes be cultured without calcium?

Keratinocyte differentiation can be induced by calcium in the culture media. As such, lowering the calcium concentration in a serum free media will greatly lower the chances keratinocytes terminally differentiating towards corneocytes. While Lonza...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay should I use matched reagents, lysate/CSE?

You need to use matched reagents in order to comply with FDA requirements for endotoxin testing. Each Lonza LAL lot is tested for functionality using United States Reference Standard EC-7. We then match this LAL lot to a lot of our Control...

What are the difference between the KGM®, KGM®-2, KGM-Gold® BulletKit®?

All three of the keratinocyte media that Lonza offers have similar basal media and supplements and all are serum free, however, slight variations and modifications have made the KGM-Gold® BulletKit® Lonza’s preferred keratinocyte...

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