Data Type
Cell Information
(857)
Citations
(4966)
FAQ
(515)
Culture Media
(61)
Category
Primary Cells and Media
(200)
Transfection
(170)
Laboratory Instrumentation
(73)
Endotoxin Detection
(54)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
3D Cell Culture
(6)
Classical Media and Reagents
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(135)
Cancer Research/Cell Biology
(77)
Immunotherapy / Hematology
(63)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Toxicology
(22)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(14)
Drug Discovery
(7)
Parasitology
(2)
Gastroenterology
(1)
+ Show All
515 results sorted by
relevance
alphabetical
newest first
oldest first
I
s
i
t
n
e
c
e
s
s
a
r
y
t
o
r
e
m
o
v
e
a
n
t
i
b
i
o
t
i
c
s
f
o
r
s
o
m
e
t
i
m
e
f
r
o
m
t
h
e
c
e
l
l
c
u
l
t
u
r
e
m
e
d
i
u
m
b
e
f
o
r
e
t
a
k
i
n
g
s
a
m
p
l
e
t
o
t
e
s
t
m
e
d
i
u
m
f
o
r
p
r
e
s
e
n
c
e
o
f
m
y
c
o
p
l
a
s
m
a
u
s
i
n
g
t
h
e
M
y
c
o
A
l
e
r
t
®
M
y
c
o
p
l
a
s
m
a
D
e
t
e
c
t
i
o
n
K
i
t
?
The MycoAlert® Mycoplasma Detection Assay was designed to “fit” in with normal tissue culturing practices. It was developed in fully supplemented culture media containing antibiotics and DOES NOT require a period of culture without antibiotics...
I
s
i
t
p
o
s
s
i
b
l
e
t
o
s
t
o
r
e
s
u
p
e
r
n
a
t
a
n
t
s
a
m
p
l
e
s
f
o
r
t
e
s
t
i
n
g
w
i
t
h
t
h
e
T
o
x
i
L
i
g
h
t
™
B
i
o
A
s
s
a
y
a
t
a
l
a
t
e
r
d
a
t
e
?
Yes, store supernatant at either -20 or -80 for up to three months.
C
a
n
m
y
c
o
p
l
a
s
m
a
g
r
o
w
o
u
t
s
i
d
e
o
f
a
c
e
l
l
?
This is an area that is up for debate. There is evidence that mycoplasma can survive in the absence of cells, and some may proliferate, but as a rule they are much happier if cells are present.
H
o
w
t
o
d
e
t
e
r
m
i
n
e
i
f
a
c
o
m
p
o
u
n
d
i
n
t
e
r
f
e
r
e
s
w
i
t
h
T
o
x
i
l
i
g
h
t
™
N
o
n
-
D
i
s
t
r
u
c
t
i
v
e
C
y
t
o
t
o
x
i
c
i
t
y
B
i
o
A
s
s
a
y
#1-2 is to determine if the media contains Adenylate Kinase (AK), the enzyme detected in Toxilight™ Assay1. Run compound in buffer with ToxiLight™ Reagents.There should be no light output.2. Run compound/Media with ToxiLight™ Reagents.If...
W
h
a
t
i
s
H
E
P
E
S
?
W
h
y
i
s
i
t
a
d
d
e
d
t
o
c
e
l
l
c
u
l
t
u
r
e
m
e
d
i
a
?
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH...
W
h
a
t
i
s
t
h
e
p
u
r
p
o
s
e
o
f
E
D
T
A
i
n
t
r
y
p
s
i
n
?
EDTA is a chelator of metal ions like calcium and magensium. It is used with trypsin because it helps bind up the divalent cations in the medium. It helps to keep the cells from sticking to each other. If its not there….you would need a higher...
W
h
i
c
h
c
e
l
l
n
u
m
b
e
r
s
s
h
o
u
l
d
I
u
s
e
w
h
e
n
r
u
n
n
i
n
g
t
h
e
T
o
x
i
L
i
g
h
t
™
N
o
n
-
D
e
s
t
r
u
c
t
i
v
e
C
y
t
o
t
o
x
i
c
i
t
y
B
i
o
A
s
s
a
y
Toxilight works much better at low cell numbers, for adherent cell lines < 10,000 in one well of a 96well plate and < 50,000 for suspension.We would normally seed 10,000 cells/well when using adherent cells to allow enough room in the well for...
C
a
n
I
u
s
e
t
h
e
V
i
a
L
i
g
h
t
™
P
l
u
s
A
s
s
a
y
a
l
s
o
,
i
f
m
y
c
e
l
l
s
a
r
e
c
u
l
t
u
r
e
d
i
n
2
4
w
e
l
l
p
l
a
t
e
s
,
6
w
e
l
l
p
l
a
t
e
s
o
r
1
2
w
e
l
l
p
l
a
t
e
s
?
To do the VialIght™ Plus Assay on larger volume tissue culture ( eg 6 well , 24 well) add the cell lysis reagent equal to half the volume in the plate ( ie 100 µl culture use 50 µl cell lysis). Wait 10 minutes for the lysis . Then follow the protocol...
I
a
m
u
s
i
n
g
t
h
e
a
c
u
t
e
l
e
u
k
e
m
i
a
H
L
-
6
0
c
e
l
l
l
i
n
e
f
o
r
C
A
R
-
T
c
e
l
l
s
e
x
p
e
r
i
m
e
n
t
s
.
D
o
y
o
u
h
a
v
e
a
t
r
a
n
s
f
e
c
t
i
o
n
p
r
o
t
o
c
o
l
f
o
r
g
e
n
o
m
e
e
d
i
t
i
n
g
?
The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors).Transfection protocols can be found...
D
o
y
o
u
k
n
o
w
i
f
t
r
e
a
t
i
n
g
c
e
l
l
s
w
i
t
h
1
0
m
M
M
g
C
l
2
w
o
u
l
d
i
n
t
e
r
f
e
r
e
w
i
t
h
t
h
e
T
o
x
i
L
i
g
h
t
™
N
o
n
-
D
e
s
t
r
u
c
t
i
v
e
C
y
t
o
t
o
x
i
c
i
t
y
B
i
o
A
s
s
a
y
?
Magnesium (Mg) is a cofactor for both luciferase and Adenylate Kinase (AK) and will speed up their reactions, but this amount of MgCl2 wouldn't make a significant difference.
PREVIOUS
PAGE
25
PAGE
26
PAGE
27
PAGE
28
PAGE
29
PAGE
30
PAGE
31
PAGE
32
PAGE
33
NEXT