Data Type
Cell Information
(858)
Citations
(4967)
FAQ
(515)
Culture Media
(61)
Category
Transfection
(3842)
Primary Cells and Media
(1963)
Serum-free and Speciality Media
(1170)
Classical Media and Reagents
(1054)
Cell Lines and Primary Cancer Cells
(644)
Bioassay
(117)
Electrophoreses and Analysis
(99)
3D Cell Culture
(91)
Laboratory Instrumentation
(75)
Endotoxin Detection
(66)
Mycoplasma Detection and Prevention
(36)
Protozoa
(16)
Live Cell Imaging
(7)
Cell Services
(4)
Uncategorized
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
+ Show All
Research Area
Cancer Research/Cell Biology
(2174)
Basic Research
(1647)
Immunotherapy / Hematology
(1589)
Neurobiology
(617)
Stem Cells
(384)
Cardiovascular
(318)
Gene Expression
(277)
Dermatology/Tissue Engineering
(265)
Molecular Biology
(179)
Gastroenterology
(139)
Regenerative medicine
(95)
Drug Discovery
(93)
Endotoxin Testing
(90)
Toxicology
(81)
Respiratory Research
(71)
Parasitology
(69)
Uncategorized
(48)
+ Show All
Platform
Nucleofector® I/II/2b
(3039)
4D-Nucleofector® X-Unit
(595)
4D-Nucleofector® 96-well Systems
(245)
4D-Nucleofector® Y-Unit
(41)
384-well HT Nucleofector® System
(17)
4D-Nucleofector® LV-Unit
(13)
Topic
Signal transduction
(1269)
Microscopy of nucleofected cells
(746)
Cellular Function
(613)
Diseases (e.g. HIV)
(600)
Cell morphology/cytoskeleton
(405)
Apoptosis
(363)
Luciferase
(330)
Genome Editing
(311)
Intercellular interaction/communication
(293)
Differentiation of nucleofected cells
(229)
Expression of dominant-negative protein
(198)
Gene therapy
(179)
Hereditary diseases
(99)
Comparison to other transfection methods
(78)
Promoter analysis
(74)
RNA transfection
(57)
Antisense
(56)
Transplantation of nucleofected cells
(32)
Virus production
(19)
Infection of Nucleofected cells
(17)
Tissue transfection
(7)
Cryopreservation
(5)
+ Show All
Species
human
(524)
mouse
(183)
rat
(66)
monkey
(15)
bovine
(11)
hamster
(10)
unicellular
(9)
porcine
(8)
chicken
(7)
canine
(6)
rabbit
(3)
baboon
(2)
cell hybrid
(2)
fish
(2)
spodoptera frugiperda (army fall worm)
(2)
Trichopulsia ni (Cabbage Looper)
(1)
drosophila melanogaster (fruit fly)
(1)
equine
(1)
feline
(1)
ferret
(1)
potoroo
(1)
sheep
(1)
+ Show All
Characteristic
Adherent
(457)
Suspension
(400)
Format
Bullet Kit
(46)
Lonza Specialty Media
(14)
Classical Media
(1)
Origin
Animal
(42)
Non-Animal
(10)
6401 results sorted by
relevance
alphabetical
newest first
oldest first
Export Citations
W
h
i
c
h
p
i
p
e
t
t
e
t
i
p
s
a
r
e
c
o
m
p
a
t
i
b
l
e
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
N
u
c
l
e
o
c
u
v
e
t
t
e
®
P
l
a
t
e
s
?
We recommend using special tips, e.g. epT.I.P.S™ (Eppendorf, Hamburg, Germany) or TallTips™ (Matrix Technologies, Hudson, NH, USA). You can contact our Scientific Support Team for a list of compatible tips, including tips for pipetting robots.
A
r
e
t
h
e
r
e
a
n
y
r
e
c
o
m
m
e
n
d
a
t
i
o
n
s
t
o
a
v
o
i
d
e
x
c
e
s
s
i
v
e
m
o
r
t
a
l
i
t
y
d
u
r
i
n
g
T
c
e
l
l
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
Try to keep the DNA amount for Nucleofection® quite low (e.g. 1 µg plasmid DNA per 100 µl reaction) since higher DNA amounts might cause increasing toxicity and mortality. It is also important to use highly purified DNA, e.g. by using Endofree™...
F
o
l
l
o
w
i
n
g
N
u
c
l
e
o
f
e
c
t
i
o
n
®
,
I
c
a
n
n
o
t
o
b
t
a
i
n
s
t
a
b
l
e
c
l
o
n
e
s
f
r
o
m
s
i
n
g
l
e
c
e
l
l
s
.
W
h
a
t
c
o
u
l
d
b
e
t
h
e
r
e
a
s
o
n
?
Some cell lines need certain cell densities to proliferate. For those cells you can try using conditioned medium for setting up limiting dilution or culturing single cells together with feeder cells.
C
a
n
I
f
r
e
e
z
e
t
h
e
9
6
-
w
e
l
l
N
u
c
l
e
o
c
u
v
e
t
t
e
®
P
l
a
t
e
s
?
Yes. Freezing of the plates at -20°C does not alter their properties.
W
h
a
t
a
n
t
i
b
i
o
t
i
c
c
o
n
c
e
n
t
r
a
t
i
o
n
s
h
o
u
l
d
I
u
s
e
f
o
r
s
e
l
e
c
t
i
n
g
s
t
a
b
l
e
t
r
a
n
s
f
e
c
t
a
n
t
s
?
We highly recommend to perform a titration for G418 in a range of 0,1 mg/ml to 1,5 mg/ml; in serum-free culture expand range down to 20 µg/ml).Choose the concentration which is 0,1 or 0,2 mg/ml above the one which shows complete cell death as...
H
o
w
m
u
c
h
D
N
A
s
h
o
u
l
d
I
u
s
e
i
n
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
®
R
e
a
c
t
i
o
n
t
o
o
b
t
a
i
n
s
t
a
b
l
e
c
l
o
n
e
s
?
You should use the amount of DNA recommended in the optimized protocol for transient transfection. If obtaining a very high number of clones is important for your experiments, you can try increasing the amount of DNA. For further tips see our...
H
o
w
c
a
n
I
i
n
c
r
e
a
s
e
c
e
l
l
v
i
a
b
i
l
i
t
y
o
f
D
e
n
d
r
i
t
i
c
c
e
l
l
s
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
DNA amount and quality are very critical for Nucleofection® of Dendritic Cells (recommended 0.5-1 µg; maximal 2 µg). LPS (lipopolysaccharides) have a strong negative effect on cell viability. Please make sure that all components you use for dendritic...
C
a
n
I
i
n
t
e
g
r
a
t
e
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
D
e
v
i
c
e
i
n
t
o
a
l
i
q
u
i
d
h
a
n
d
l
i
n
g
s
y
s
t
e
m
?
Yes. The 96-well Shuttle® Device is designed in such a way that it is addressable by robot grippers. Furthermore, the software contains the required interface to be connected with the LHS software. However, the practical implementation for each...
I
w
a
n
t
t
o
s
t
i
m
u
l
a
t
e
H
u
m
a
n
T
c
e
l
l
s
p
o
s
t
N
u
c
l
e
o
f
e
c
t
i
o
n
®
.
A
r
e
t
h
e
r
e
a
n
y
p
r
e
c
a
u
t
i
o
n
s
t
o
k
e
e
p
i
n
m
i
n
d
?
After Nucleofection®, wait at least four hours before stimulation. Stimulating immediately after Nucleofection® may lead to increased cell mortality and poor activation of cells. For more details on the stimulation of Human T cells post...
C
a
n
I
u
s
e
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
2
b
S
o
l
u
t
i
o
n
s
a
n
d
O
p
t
i
m
i
z
e
d
P
r
o
t
o
c
o
l
s
w
i
t
h
t
h
e
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
®
X
-
U
n
i
t
o
r
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
S
y
s
t
e
m
?
No. The 4D-Nucleofector and 96-well Shuttle® System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle® Solutions are available for primary cells. For cell lines, three...
PREVIOUS
PAGE
274
PAGE
275
PAGE
276
PAGE
277
PAGE
278
PAGE
279
PAGE
280
PAGE
281
PAGE
282
NEXT