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How is the B-ALI™ Media System performance tested?

The media system is performance tested with our pre-screened NHBE cells and that system is guaranteed to form ciliated, mucous-producing cells after 3 weeks of culture.
Missing: stable lines

What is the amount of DNA needed per well when using the 96-well Shuttle® System?

The amount depends on the cell type and on the DNA construct. As a rule of thumb 400 ng of DNA per well should be applied. However, for some cells or constructs even 100 ng may be sufficient.
Missing: stable lines

What is the advantage of the Nucleofector® Technology over common electroporation (systems)?

High cell viability and high transfection efficiency with the Nucleofection® Technology are the most prominent features. Nucleofection®, i.e., the transfer of DNA into the nucleus and not only the cytosol, is essential when working...
Missing: stable lines

What is the best way to centrifuge Human Hepatocytes prior to Nucleofection™?

In order to avoid cell compaction in the pellet and difficulties in cell resuspension, we recommend using round bottom 2 ml vials or 50 ml BD Falcon™ tubes for all centrifugation steps prior to Nucleofection™.
Missing: stable lines

What are mycoplasmas?

...and Acholeplasma species from human, bovine and swine have been isolated from cell culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale, M.arginii, M.fermentans, M.salivarum, M.hyorhinis and A.laidlawii).Mycoplasmas are widespread...
Missing: stable lines

Can serum be used with Lonza's SAEC’s?

No. Serum will induce squamous differentiation of primary airway epithelial cells. Our SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit CC-3118 does not contain FBS or any other serum.
Missing: stable lines

Why do plasmids which contain IRES sequences often have lower transfection efficiency?

...Since the downstream gene is usually GFP, the expression level of the GFP reporter will be lower than would be normally seen when compared to plasmids without an IRES-sequence. Thus the percentage of cells expressing detectable GFP fluorescence...
Missing: stable lines

I transfected neurons using the 4D-Nucleofector® Y-Unit. After Nucleofection®, there were precipitates in my culture. What can I do?

Most likely there was too much Nucleofector® Solution left before medium addition. Please try to aspirate solution after Nucleofection® as complete as possible without disturbing cell adherence, but add medium quickly to avoid cells...
Missing: stable lines

How does TheraPEAK 293-GT® Media System compare to other AAV production media on the market?

Download our application note or product sheet to see cell growth, cell viability, viral titer and viral capsid data for TheraPEAK 293-GT® Media System as compared to the industry leading complete production kit and the industry leading media...
Missing: stable lines

What is the shelf life of the PyroCell® MAT System Kits?

Except for the pMAT cells, the shelf life of each component is indicated on the vial label or the kit box label. The pMAT cells can be used for up to 6 months as of receiving.PyroCell® MAT System Kits component should be used beyond its expiry...
Missing: stable lines
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