The EBM™ and EBM™-2 basal media are essentially identical. The difference between the EGM™ and EGM™-2 growth media (or the EGM™-MV and EGM™-2MV growth media) are in the supplements.

EGM™ and EGM™-MV growth media will contain the undefined reagent Bovine Brain Extract (BBE).

In EGM™-2 and EGM™-2MV growth media, the BBE has been removed and replaced by the defined growth factors fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), Insulin-like growth factor (R3-IGF-1), and epidermal growth factor (hEGF).

Cells tend to proliferate better in the EGM™-2 and EGM™-2MV growth media versions, however some customers have indicated that the original EGM™ and EGM™MV growth media works better for VEGF, FGF and angiogenesis research. Additionally, the EGM™ and EGM™MV growth media works better for bovine derived endothelial cells.

• In summary:

  • EGM™ / EGM™-MV : contain BBE (undefined component).

  • EGM™-2 / EGM™-2MV : use defined growth factors (no BBE).

  • EGM™-Plus : enhanced version of EGM™, optimized for HUVECs, no exogenous VEGF.

  • –MV versions : higher serum concentration (5%) for microvascular cells.

The EGM-MV BulletKit CC-3125 has been discontinued in 2023