No. SDS has a negative influence on Adenylate Kinase (AK) activity.
SDS is a cationic detergent, it is a very powerful protein denaturing agent, you will be aware of its use in SDS-PAGE for those reasons. ToxiLight™ works by measuring the activity of the enzyme adenylate kinase released from cells undergoing necrosis, this will obviously be denatured by the SDS.
ToxiLight™ is a bioluminescent based assay which uses the firefly enzyme luciferase, the luciferase will also be denatured by the SDS. You will therefore see exactly what you have done, increasing the SDS concentration, while actually inducing more cell death, will result in lower RLUs from the assay that would seem to indicate the exact opposite. So SDS is not the best choice for the combination with Toxilight™ or LDH. The alternative detergents are like camptothecin or ideally our Toxilight™ 100% lysis control set (order number LT07-517).