Search Results

What is the difference between a Tissue Acquisition Number (TAN) and a lot/batch number?

Each time Lonza receives a tissue sample, the entire tissue sample is assigned a unique Tissue Acquisition Number (TAN).However, each collection/production of final, prepared product will be assigned a unique lot/batch number.For many productions,...

Can I plate cells at a higher initial concentration than the Clonetics™ and Poietics™ plating protocol recommends? What is the minimum dilution of DMSO most cells can tolerate?

Lonza's recommended plating density for primary cells is established for two main reasons: To ensure residual DMSO is sufficiently diluted while cells are initially attaching and To allow a maximum number of population doublings per passage. With...

What is the principle behind the ViaLight™ Plus Cell Proliferation and Cytotoxicity Assay?

ViaLight™ Plus BioAssay Kits use bioluminescence catalysed by the firefly luciferase enzyme to measure intracellular ATP. Since ATP is central to the metabolism of all cells, the intracellular concentration is very precisely...

Do intracellular ATP levels differ between different cell types?

Healthy cells in culture contain maximal amounts of ATP and a wide range of metabolic stimuli do not increase these ATP levels. Any increase in the ATP concentration of a culture can therefore be associated with an increase in the number of...

Can the ViaLight™ Plus Kits be used for both proliferation and cytotoxicity assay

Yes. Since the ATP levels are precisely maintained in healthy cells, the level of ATP accurately reflects the number of viable cells in a culture. In response to a proliferative signal (e.g. a growth factor) the number of cells in a...

What instrumentation is needed to run the ViaLight™ Plus Assay?

To achieve optimal performance and convenience a microplate luminometer should be used. If reagent injectors are available on the instrument, these should be used since they enable full automation of the assay. If reagent injectors are...

Does Lonza have a suggested TRAP kit for testing Osteoclast differentiation/functionality?

The TRAP kit Lonza used in the past when testing Osteoclast differentiation/functionality is the Acid Phosphatase, Leukocyte (TRAP) Kit from Sigma-Aldrich (Sigma-Aldrich catalog no. 386A). Now, we are using our OsteoLyse Assay Kit PA-1500 for testing...

How can I submit references to Lonza for inclusion in the Lonza Citation Database?

Lonza welcomes submissions of published scholarly article from researchers detailing the use of Lonza products. Please email reference submissions to Scientific.Support.eu@lonza.com. Please indicate if you would like to make the reference publically...

What plate format is the protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for?

The protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for plating cells into a 96-well plate.

Where can I obtain the Safety Data Sheet (SDS) for a Lonza product?

Safety Data Sheet (SDS) [also called Material Safety Data Sheet (MSDS)] for all Lonza research products can be obtained by going to the product page.From any page on the Lonza Bioscience website (https://bioscience.lonza.com/), search for the product...

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