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Can Lonza’s HSMM or SkMC cells be maintained/subcultured/cryopreserved as myotubes after they have been differentiated?

The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts. Differentiated HSMM myotubes can be maintained in...

Why does Lonza suggest multiple concentrations of Hydrocortisone-21-hemisuccinate and ß-Glycerophosphate when differentiating Normal Human Osteoblasts (NHOst)?

While both hydrocortisone-21-hemisuccinate and ß-glycerophosphate are necessary when differentiating Lonza’s Normal Human Osteoblasts (NHOst), Lonza has noted that some lots of NHOst may be more sensitive to the concentration of...

Why must Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) be re-differentiated to express collagen?

Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) are differentiated cells which express collagen when they are initially isolated, however, they lose their differentiated phenotype after serial expansion in monolayer culture so that by the 2nd...

With regards to mesenchymal stem cells, what are "Dexter cultures"?

Dexter culture refers to the method of isolating stem cells proposed by Dexter et al in their 1977 paper: Dexter TM, Allen TD, Lajtha LG. Conditions controlling the proliferation of haemopoietic stem cells in vitro. JCell Physiol 1977; 91: 335–344....

Will Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) express collagen Type X? Are Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) hypertrophic following re-differentiation?

Lonza guarantees that the Normal Human Articular Chondrocytes (NHAC-kn) will express collagen Type II and sulfated proteoglycans (can be stained with Alcian Blue) upon re-differentiation. Lonza’s recommended differentiation protocol does induce...

384-well Nucleofector® System: Can I apply 384 different programs to a 384-well Nucleocuvette® Plate?

Yes, you can. Each well is pulsed individually.

Where are Lonza's Bronchial Smooth Muscle Cells isolated from?

Lonza’s Bronchial Smooth Muscle Cells (both normal and diseased) are isolated from the bronchial tubes. The region used is around the bifurcation of the bronchial tubes to the lungs and small sections of the bronchia and trachea just off of the...

Do you also sell the boxes or cases for the Nucleofector® Core, X, Y and LV Units?

Yes, see this list of part codes for the different types of transport boxes: Part code: AAK-1001Items: 4D-Nucleofector® Demo Suitcase (2 units)Suited for: Core + X or Core + Y or X + YPart...

Is the classical Lucetta® Luminometer compatible with MycoAlert® PLUS Assay?

Yes. You can use the MycoAlert® mode of the classical Lucetta® Luminometer AAL-1001 for running MycoAlert® PLUS Assay. However, as the MycoAlert® mode was programmed based on standard MycoAlert® Assay and has not be adapted...

Where are Lonza's normal Adipose Derived Stem Cells isolated from?

Lonza's normal (non-diabetic) Adipose Derived Stem Cells (ADSCs) are isolated from lipoaspirate obtained from liposuction surgeries. As liposuction surgeries typically pool lipoaspirate from multiple locations, a single isolation source is not able...

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