Search Results

Does the recommended media for each of the diabetic cells contain glucose?

The recommended media does contain glucose. Please contact Scientific Support for glucose concentrations.

When is the ideal time to study Lonza’s Human Knee Articular Chondrocytes (NHAC-kn) after re-differentiation?

For maximum re-differentiation and therefore maximum collagen production, Lonza’s Human Knee Articular Chondrocytes should be studied within the first passage after re-differentiation. Serial expansion in monolayer culture after re-differentiation...

How would you describe the morphology of Lonza’s Normal Human Osteoblasts (NHOst)?

Lonza’s Normal Human Osteoblasts (NHOst) generally appear fibroblastic. They are cuboidal in lower densities and become spindle shaped as they become confluent.

Is the viability of cells from diseased or diabetic donors less than cells from normal donors?

We guarantee > 70 % viability for cells from both diseased/diabetic donors and normal donors.

What is the difference between the EBM™ and EBM™-2 Basal Media? Can the EBM™-2 Basal Media be substituted with the EBM™ phenol red free Basal Media?

The EBM™ and EBM™-2 Basal Media are essentially identical. The major difference between the EBM™ and EBM™-2 Basal Media is the concentration of phenol red (the EBM™-2 Basal Media has about 1/10th the amount of phenol red as the EBM™ Basal Media). The...

What is the difference between the EGM™ and EGM™-2 growth media? What is the difference between the EGM™-MV and EGM™-2MV growth media?

The EBM™ and EBM™-2 basal media are essentially identical. The difference between the EGM™ and EGM™-2 growth media (or the EGM™-MV and EGM™-2MV growth media) are in the supplements.EGM™ and EGM™-MV growth media will contain the undefined reagent...

What is the percentage of purity of Lonza’s Normal Human Osteoblasts (NHOst) cells?

It is not easy to put an exact number to the purity of the NHOst populations because the tests used are all functional assays: alkaline phosphatase production and bone mineralization, which unless you have synchronized cultures, not all the cells are...

Are Lonza’s Normal Human Osteoblasts (NHOst) contact inhibited?

No, Lonza’s Normal Human Osteoblasts (NHOst) are not contact inhibited and tend to grow on top of each other after becoming confluent.

Do we have any Diseased Human Bronchial/Tracheal Epithelial Cells (DHBE) screened for Air Liquid Interface (ALI) differentiation using the B-ALI™ BulletKit™?

Yes. All our DHBEs are also offered in a version screened for ALI differentiation. The lots which do pass this additional ALI testing are electronically designated by adding an “S” to the end of the original catalog number.

Why does Lonza recommend two different concentrations of the B-Glycerophosphate in the Normal Human Osteoblasts (NHOst) differentiation protocol?

Lonza R&D has found some NHOst cell strains to be more sensitive to B-Glycerophosphate than others. While it is necessary to differentiate, at too high of concentrations the cells will lift off dish, curl up and die. As such, Lonza recommends...

Data Type

Category

Research Area