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When running a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection assay why should I use glass accesory products instead of plastic?

Endotoxin adheres to plastic surfaces more strongly than to glass surfaces. Therefore, we recommend that you use only borosilicate glass dilution tubes when preparing your Control Standard Endotoxin (CSE) dilutions. In some cases, certain plastics...

Why are Lonzas adult melanocytes cultured with Endothelin-3 (ET-3) while the neonatal melanocytes are not cultured with this reagent?

Adult melanocytes typically have a very slow doubling time, especially in comparison to neonatal melanocytes. Endothelin-3 (ET-3) is added to adult melanocytes only to improve the doubling time and, in the process, help to maintain the cells as...

What is the distance between the two combs on Lonza's Reliant Gel, catalog number 57236?

From the bottom of one well to the top of the next, the distance is 31mm.

Are Lonzas human mammary epithelial cells tested for estrogen receptor response?

Unfortunately, Lonza has not thoroughly investigated the estrogen receptor response/response to estrogen of the mammary epithelial cells being isolated in the HMEC product.

When running a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection kit why is it important to vortex my control standard endotoxin (CSE) dilutions?

Endotoxin will adhere to glass surfaces, but this can be counteracted with proper vortexing to ensure that the solution you aliquot into your reaction tubes or microplate has the proper EU/ml concentration. As our package inserts state, the CSE vial...

Can plasma or serum be run on Lonza's FlashGel System without prior purification?

No. The DNA or RNA will need to be extracted and purified before the samples can be loaded and run on the FlashGel™ Cassettes.

How are Lonza's mononuclear cells isolated?

Lonza's mononuclear cells are isolated from bone marrow, cord blood, or peripheral blood via density gradient centrifugation

What cells make up the mononuclear cell population?

Mononuclear cells are any blood cells with a rounded nucleus including lymphocytes (T-cells, B-cells, and Natural Killer [NK] Cells), monocytes, and dendritic cells.

Do the FlashGel Visualization Glasses protect against UV light?

Yes. They block UV exposure up to 400nm and “blue light” between 400 and 510nm. They meet OSHA requirements and ANSI Z87.1.

How are Lonza's CD14+ monocytes isolated?

Lonza's CD14+ monocytes are isolated first by obtaining mononuclear cells from peripheral blood via density gradient centrifugation. The CD14+ cells are then isolated form the mononuclear cell population using either positive or negative...
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