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The ReagentPack™ Subculturing Reagents Kit is composed of three components: - 100 ml of HEPES Buffered Saline Solution, - 100 ml of Trypsin/EDTA- 100 ml of Trypsin Neutralizing Solution (TNS).
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No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...
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No, we do not recommend heat inactivating the FBS provided in the SingleQuots™ Kit. Heat inactivating the serum can alter cell performance and denature essential components in the serum.
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We do not recommend reusing the conductive polymer cuvettes as performance decreases when cuvettes are used more than once.
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The SingleQuots™ Kit provided in the BulletKit™ Media have been premeasured for the bottle of basal medium also provided in the kit. For most BulletKit™ Media, simply thaw the SingleQuots™ and add them to the bottle of basal medium. The complete...
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This depends on the cell type you are working with. In our Optimized Protocols the cell numbers range from 2.5 x 10^4 cells (mouse DC) up to 1x 10^6 cells (human T cells). Detailed information is provided in our 4D-Nucleofector® Optimized Protocols.
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Please be aware that cryopreservation may compromise cell quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™ Cells that have been cryopreserved outside of Lonza. To avoid loss of cells and forfeiture of your...
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Cells might have been disturbed or ran dry during pipetting steps. Please perform liquid removal and additions well-by-well. Avoid leaving neurons without any liquid coverage (medium or Nucleofector® Solution). To keep a small liquid film on the...
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For most cell types, Lonza recommends the Trypsin/EDTA provided as one of the components in our ReagentPack™ Subculturing Reagents Kit (CC-5034).The concentration of this solution is proprietary.
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No, each well can be addressed separately. You may apply up to 16 different programs to one 16-well Nucleocuvette® Strip.
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