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How can I best clean used dipping electrodes and store unused for later use? Can I wash used dipping electrodes in an ethanol bath?

After withdrawing the dipping electrode array from the plate you can wipe off residual liquid with a tissue. Alternatively, you can wash used electrodes with ethanol which won't damage the electrodes. To avoid cross-contamination of non-used...

What are Lonza's human Mesenchymal Stem Cells (PT-2501) cryopreserved in?

The cryopreservation medium is: 85% MSCBM 10% DMSO 5% Human Serum Albumin (w/v)

Can the ProSieve Molecular Weight Markers be used on native gels?

No, they are suitable for use on denaturing gels only.

How can I find out if other 24-well culture plates than Greiner or Nunc are compatible to the dipping electrode array for adherent Nucleofection® using the 4D-Nucleofector® Y Unit?

Please find attached a list with all plate types that have been checked for compatibility so far. If your plate type is not on this list, please contact our Scientific Support Team we can investigate compatibility.

What is the percentage of CD34+ cells in the bone marrow mononuclear cell population?

CD34+ cells make up about 2% of the population in bone marrow mononuclear cells.

I am using your ProSieve Color Protein Markers and the molecular weights (MW) of my protein of interest is not what I expected when compared to the ProSieve Markers. Why is this?

The proteins in our ProSieve™ Color Markers are made of proteins that are individually prestained using colored dyes.The level of dye bound can affect the migration of the protein and this can vary slightly lot to lot. Therefore, it is important to...

Can I expand the mononuclear cells, CD34+ progenitors, without differentiating them?

No, the cells will proliferate and differentiate simultaneously upon being placed in culture. Varying the cytokine cocktail can influence the differentiation and proliferation of the cells.

Can Lonza's Human Adipose-Derived Stem Cells (hADSC) (PT-5006) be expanded prior to differentiation?

Lonza's human adipose derived stem cells (ADSC) are guarantee 5 passages prior to differentiation based on our existing data.

What is a BulletKit® Media?

A BulletKit® Media contains a bottle of basal medium and a SingleQuots® Kit. It is possible to purchase each of these components separately. Once the basal medium is supplemented with the SingleQuots® Kit, it becomes complete growth medium.

I transfected neurons using the 4D-Nucleofector® Y unit. During analysis I observed areas with low or no cell density, even in my no pulse control sample.

Cells might have been disturbed or ran dry during pipetting steps. Please perform liquid removal and additions well-by-well. Avoid leaving neurons without any liquid coverage (medium or Nucleofector® Solution). To keep a small liquid film on the...
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