Search Results

What wavelength will excite the stain in the FlashGel™ Cassettes? Can I use a UV transilluminator?

The FlashGel™ Dock has a blue light transilluminator that excites the fluorescent dye at 450 nm. The stain has a secondary excitation peak in the UV range (i.e., 302 nm and 312 nm). Therefore, the bands can also be detected on a UV transilluminator.

Where are Lonza’s dermal cells isolated from?

Lonza is offering different dermal cell types, which are either isolated from adult or from neonatal tissue.Lonza’s adult dermal cells (including keratinocytes, microvascular endothelial cells, fibroblasts, and melanocytes) are usually isolated from...

Where are Lonza’s PrSC cells isolated from? What cells will be in the PrSC population?

Lonza’s Prostate Stromal Cells (PrSC) are isolated from the stroma of the prostate. Lonza’s Prostate Stromal Cells (PrSC) are a mixed population of cells, however, the majority of the cells in this population are fibroblastic in origin. It is not...

What is the maximum well volume for the FlashGel™ Cassettes?

The maximum volume per well for the standard DNA and RNA cassettes is 5 µl. The recovery cassettes are designed to hold more and can be filled to 12 µl.

Where are Lonza’s PrEC cells isolated from?

Lonza obtains our Lonza’s Prostate Epithelial Cells (PrEC) from the glandular epithelium (parenchymal portion) of the prostate which spans the entire prostate and not from one specific region.

What happens if I load too much or too little sample on the FlashGel® Cassette?

The FlashGel® System is very flexible. It is best to experiment with the system and adjust the levels to optimize your results.Optimal DNA load levels are 5-20 ng per band in a 5 µl load.DNA fragments = 5 ng are visible on the...

How old are the donors for Lonza’s dermal cells from neonatal donors?

The neonatal foreskin tissue used to isolate Lonza’s dermal cells from neonatal donors is almost always collected within the first few days after birth, and at most a week or two after birth. At most U.S. hospitals, circumcisions are performed before...

Where are Lonza’s PrSMC cells isolated from?

Lonza’s Prostate Smooth Muscle Cells (PrSMC) are isolated from the stroma and the vasculature of the prostate. Unfortunately, there is no way to distinguish between smooth muscle cells from the stroma and smooth muscle cells from the vasculature and...

At what voltage do I need to run the FlashGel® Cassettes? Can I speed up or slow down the run?

We recommend that the FlashGel® DNA cassettes be run at 275 V and RNA cassettes at 225 V. We do not recommend running a gel at a higher voltage than 275 V.Runs at lower voltage for longer time periods may improve separation of larger fragments...

How do I promote keratinocyte terminal differentiation towards corneocytes?

Keratinocyte differentiation can be induced by increasing the concentration of calcium and/or by adding serum to the media. Cells should be initially plated using the standard culture media and conditions and allowed ample time to recover from...

Data Type

Category

Research Area