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No, the cells secrete fluid at the apical side but also moisture is drawn through the cell layer from the basal layer.
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No, the cells will proliferate and differentiate simultaneously upon being placed in culture. Varying the cytokine cocktail can influence the differentiation and proliferation of the cells.
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Lonza’s Bronchial/Tracheal Epithelial Cells (both with and without Retinoic Acid; both normal and diseased) are isolated from the epithelial cells that line the airway of the bifurcation of the lungs and small sections of the bronchia and trachea...
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?
Lonza's human adipose derived stem cells (ADSC) are guarantee 5 passages prior to differentiation based on our existing data.
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The cryopreserved epithelial cells, that are screened and guaranteed for differentiation in B-ALI™ (Bronchial Air Liquid Interface) are in P1, the cells plated in transwells are P2. We do not recommend expansion of the cells before...
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d
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m
?
Lonza’s Small Airway Epithelial cells (both normal and diseased) are isolated from the distal portion of the lung in the 1 mm bronchiole area. These cells are isolated from airway tubules 1mm in diameter and smaller. Anything with a diameter greater...
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-
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e
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d
c
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l
l
s
?
All of Lonza’s airway epithelial cells receive standard quality control testing in monolayer culture. After cells have passed Lonza’s initial monolayer quality control testing, a few lots receive additional quality control testing to monitor...
W
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t
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s
a
B
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t
K
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®
M
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a
?
A BulletKit® Media contains a bottle of basal medium and a SingleQuots® Kit. It is possible to purchase each of these components separately. Once the basal medium is supplemented with the SingleQuots® Kit, it becomes complete growth medium.
H
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™
M
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d
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a
K
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t
k
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t
p
r
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v
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?
1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.
W
h
a
t
a
r
e
S
i
n
g
l
e
Q
u
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t
s
™
K
i
t
s
?
A SingleQuots™ Supplements and Growth Factor Kit contains several vials that may include growth factors, FBS, and antibiotics in pre-measured aliquots - designed to be added to a bottle of basal medium. The concentrations of these factors are...
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