Data Type

Category

+ Show All

Research Area

+ Show All
94 results sorted by

For hepatocytes, is plating density a concern post Nucleofection®?

Yes, the optimized protocols for the standard Nucleofector® recommend the use of 6 well plates. Plating into a 12-well plate will pose a problem as less cells will attach at high densities.
Missing: mycoalert

Why can’t I obtain a Certificate of Analysis (COA) for a MycoAlert®/MycoAlert® PLUS/ToxiLight®/ViaLight® Kit via your Webpage-tool?

Lonza’s luminescent assays (including MycoAlert® Mycoplasma detection assay, MycoAlert® PLUS Mycoplasma detection assay, MycoAlert® Control set, ToxiLight® Non-Destructive Cytotoxicity Assay, and ViaLight® Plus Cell Proliferation...
Missing: plate

Can I use the ViaLight™ Plus Assay also, if my cells are cultured in 24well plates, 6well plates or 12 well plates?

To do the VialIght™ Plus Assay on larger volume tissue culture ( eg 6 well , 24 well) add the cell lysis reagent equal to half the volume in the plate ( ie 100 µl culture use 50 µl cell lysis). Wait 10 minutes for the lysis . Then follow...
Missing: mycoalert

Is it possible to quantify the mycoplasma with the MycoAlert® Mycoplasma Detection Assay?

No, the MycoAlert® Mycoplasma Detection Assay (as well as the MycoAlert® PLUS Mycoplasma Detection Assay) is not quantitative; it has been designed to give a simple Yes or No answer, pertaining to the presence of Mycoplasma.
Missing: plate

What temperature should the MycoAlert® Assay be run at?

Optimal assay working temperature for all components is 18°C-22°C (for MycoAlert® Assay) and 20-22°C (for MycoAlert® PLUS Assay). In case room temperature significantly deviates from this optimal temperature range, it is recommended to use...
Missing: plate

Do bacteria lead to false positive MycoAlert® Results?

...is characterized by rapid appearance of turbidity in cell culture media. One of the dangers of mycoplasma infection is that they do not produce turbidity, hence the need for a test such as the MycoAlert® Assay....
Missing: plate

Does the MycoAlert® Assay recognize the whole spectrum of mycoplasma?

The mycoplasma specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in cell culture). 95...
Missing: plate

When should I use the MycoAlert® Assay Control Set?

The positive control should be always included as reference for a positive signal. It serves as control for the performance of the MycoAlert® Assay and the MycoAlert® PLUS Assay and the luminescence measurement.
Missing: plate

For Lonza’s MycoAlert® Mycoplasma Detection Assays is it possible to convert the B/A value of positive control included in the MycoAlert® Assay Control Set to CFU of mycoplasma?

The MycoAlert® and MycoAlert® PLUS Assay are not quantitative so you cannot relate the ratio to an actual value. Different mycoplasma species will give different ratios due to different levels of expression of the enzymes we measure...
Missing: plate

Do you have recommendations for the type of samples used for the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit? And do you have recommendations related to sample storage?

The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Assay require the use of cell free supernatant only! Cells MUST be removed at 200xg for 5 mins.For optimal assay performance, supernatant should be tested...
Missing: plate
PAGE 2

Privacy | Legal | About Lonza

© Lonza. All rights reserved.