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Co-culture protocol for Hepatocytes and Non-Parenchymal Cells/NPC (2D) 

If you are searching for a 2D co-culture protocol for Hepatocytes and Non-Parenchymal Cells (NPC), please find our co-culture protocol on Hepatocyte and Non-Parenchymal Cell (NPC) 2D Co-CultureAnd in case you want to work high-throughput, here...
Missing: stable linesGuideline

Can I store my cryopreserved primary cell ampule at –80°C?

No, cryopreserved cells should be stored in liquid or vapor phase nitrogen. Storing the cells at higher temperatures can irreversibly damage the cells.
Missing: stable linesGuideline

What is the component labeled "MCGS" in the mesenchymal stem cell growth and differentiation media?

Mesenchymal Cell Growth Supplement (MCGS) is 100% fetal bovine serum prescreened to neither induce nor prevent differentiation of human bone marrow derived stem cells PT-2501
Missing: stable linesGuideline

Are the Clonetics™ embryonic rat/mouse neuronal cells from a single donor or pooled?

The cells are pooled from whole litters, including both males and females.
Missing: stable linesGuideline

Do you have any instructions available for using a hemocytometer for cell counting?

Yes. Our technical guideline "Cell Counting and Determination of Viability via Hemocytometer" can be found as associated pdf file in the link below.
Missing: stable linesGuideline

Are Lonza’s human mammary epithelial cells tested for estrogen receptor response?

Unfortunately, Lonza has not thoroughly investigated the estrogen receptor response/response to estrogen of the mammary epithelial cells being isolated in the HMEC product.
Missing: stable linesGuideline

What concentration of Trypsin/EDTA do you recommend for subculturing primary cells?

For most cell types, Lonza recommends the Trypsin/EDTA provided as one of the components in our ReagentPack™ Subculturing Reagents Kit (CC-5034).The concentration of this solution is proprietary.
Missing: stable linesGuideline

What medium and which cytokines are recommended for Natural Killer (NK) cell culture?

For the cultivation of Natural Killer (NK) cells we recommend using the X-VIVO 15 medium.In regard to cytokines, this varies depending upon the application, but many cited publications indicate using IL-2 for activation and longer term culture.
Missing: stable linesGuideline

How many mycoplasma species exist? Which are the usual suspects in cell culture?

More than 180 species have been identified of which 20 distinct Mycoplasma and Acholeplasma species from human, bovine and swine have been isolated from cell culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale...
Missing: stable linesGuideline

What is the shelf-life for all the media associated with Human mesenchymal stem cells?

The shelf-life of the MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ (PT-3001) once the SingleQuots Kit is added is 30 days. For the differentiation kits:hMSC differentiation BulletKit™ – osteogenic (PT-3002)hMSC differentiation...
Missing: stable linesGuideline
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