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Can I store my cryopreserved primary cell ampule at –80°C?

No, cryopreserved cells should be stored in liquid or vapor phase nitrogen. Storing the cells at higher temperatures can irreversibly damage the cells.
Missing: stable linesGuideline

What is the percentage of purity of Lonza’s Normal Human Osteoblasts (NHOst) cells?

It is not easy to put an exact number to the purity of the NHOst populations because the tests used are all functional assays: alkaline phosphatase production and bone mineralization, which unless you have synchronized cultures, not all the cells...
Missing: stable linesGuideline

Co-culture protocol for Hepatocytes and Non-Parenchymal Cells/NPC (2D) 

If you are searching for a 2D co-culture protocol for Hepatocytes and Non-Parenchymal Cells (NPC), please find our co-culture protocol on Hepatocyte and Non-Parenchymal Cell (NPC) 2D Co-CultureAnd in case you want to work high-throughput, here...
Missing: stable linesGuideline

What is the component labeled "MCGS" in the mesenchymal stem cell growth and differentiation media?

Mesenchymal Cell Growth Supplement (MCGS) is 100% fetal bovine serum prescreened to neither induce nor prevent differentiation of human bone marrow derived stem cells PT-2501
Missing: stable linesGuideline

What growth factor is pertinent in chondrogenic differentiation of mesenchymal stem cells?

TGF-B3 has worked best in our hands so that is what we recommend.
Missing: stable linesGuideline

What is the primary differentiation reagent for chondrogenic differentiation of mesenchymal stem cells?

Lonza recommends the addition of TGF-B3 (PT-4124) to the Chondrogenic Differentiation Media (PT-3003) to promote chondrogenic differentiation of MSCs. Please see the MSC differenation protocol for complete details on differentiating MSCs down a...
Missing: stable linesGuideline

Where are Lonza’s NHOst CC-2538 cells isolated from?

Lonza obtains our Normal Human Osteoblasts (NHOst) from the spongy section of the bone head of ribs, femur, radius, fibula or tibia.If you would like to know, from which specific bone your lot of NHOst has been isolated, just contact our Scientific...
Missing: stable linesGuideline

Are the Clonetics™ embryonic rat/mouse neuronal cells from a single donor or pooled?

The cells are pooled from whole litters, including both males and females.
Missing: stable linesGuideline

Do you have any instructions available for using a hemocytometer for cell counting?

Yes. Our technical guideline "Cell Counting and Determination of Viability via Hemocytometer" can be found as associated pdf file in the link below.
Missing: stable linesGuideline

Are Lonza’s human mammary epithelial cells tested for estrogen receptor response?

Unfortunately, Lonza has not thoroughly investigated the estrogen receptor response/response to estrogen of the mammary epithelial cells being isolated in the HMEC product.
Missing: stable linesGuideline
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