If you are searching for a 2D co-culture protocol for Hepatocytes and Non-Parenchymal Cells (NPC), please find our co-culture protocol on Hepatocyte and Non-Parenchymal Cell (NPC) 2D Co-Culture
And in case you want to work high-throughput, here is also a specific protocol Hepatocyte and Non-Parenchymal Cell (NPC) 2D High-Throughput Co-Culture
Furthermore, please find our White Paper showing informative data on Hepatocytes and Non-parenchymal Cells - Development and Optimization of a Comprehensive Co-culture