Q:

What selection markers can I use for the generation of stable cell lines?

A:

The most commonly used marker is the neomycin phosphotransferase (neo) gene that confers resistance to G418 to eukaryotic cells.

Other markers are Puromycin, Hygromycin, Zeocin, or the HPRT gene that can be used in HPRT-deficient cells.

Related Literature:
Lonza_BenchGuides_Guideline_for_Generation_of_Stable_Cell_Lines__Technical_Reference_Guide.pdf
Categories:
Transfection
Research Areas:
Gene Expression

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