Q:

How long does it take to obtain stable transfectants?
A:

Depending on individual cell type and doubling rate, selection of stable transfectants will take between 7 and 28 days.

Expansion and characterization of single cell clones will take several weeks in addition.
Related Literature:
Lonza_BenchGuides_Guideline_for_Generation_of_Stable_Cell_Lines__Technical_Reference_Guide.pdf
Categories:
Transfection
Research Areas:
Gene Expression

You may also want to know:

Generation of a stable cell line: A positive control plasmid works, but I cannot obtain stable clones with my construct of interest. What could be the reason?
What selection markers can I use for the generation of stable cell lines?
Is your pmaxGFP control vector supplied with the Nucleofector® Kits suitable for stable expression?
How many stable clones will I get from one transfection?
Why is the Nucleofector® Technology ideal for protein production from stable clones ?
In stable cell line generation, I have a very high transfection efficiency, but most of my cells die during selection. Is this to be expected?
Following Nucleofection®, I cannot obtain stable clones from single cells. What could be the reason?
How long is the MycoAlert® Mycoplasma Detection Assay signal stable?
What antibiotic concentration should I use for selecting stable transfectants?
How much DNA should I use in my Nucleofection® Reaction to obtain stable clones?
See More

Privacy | Legal | About Lonza

© Lonza. All rights reserved.