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PyroTec Pro: Is it possible to reload the instrument during a run with reagents, consumables, samples? Can you continuously load? Is an automatic retest supported?

After the last microplate is placed in the reader and control is transferred to the WinKQCL™ Software to begin reading the plate, the instrument’s work is complete. At that time, it would be possible to start preparing the deck for the next run....

PyroTec Pro: Has any stability testing been performed for the endotoxin and lysate that stay on the instrument before use?

Our data indicate that the reagents should be placed on the deck prior to running the automated assay. This would be similar to the methodology when performing the assay on the bench. Customers should follow procedures similar to those in a manual...

PyroTec Pro: How long does the assay take, and can an assay be run overnight?

Typical runtime from start of the automated microplate setup to completion of WinKQCL™ Software reading the plate for a single assay is 1.5 hours – 2.5 hours, depending on the number of samples and the complexity of the dilutions. Therefore, a...

How long does it take to perform the PyroTec Pro installation, operational and performance (IOPQ)?

There are many components that need to be installed and validated. Depending on the customizations needed, estimated time for installation and operational qualification is one week.

Pathogenic human coronaviruses such as severe acute respiratory syndrome CoV (SARS-CoV) predominantly infect lower airways and can cause fatal pneumonia. What kind of products you have to support this research area?

SARS-Cov-2 enters target cells through the (angiotensin converting enzyme) - receptor leading to infections of ACE-2 expressing target cells1 . ACE2 is an important regulator of the immune response and inflammation. It is expressed in...

What components make up the PyroTec™ PRO robotic solution?

PyroTec™ PRO is comprised of a liquid handling robot developed from the Tecan Freedom EVO® 150 Series with Lonza’s proprietary dynamic scripting for endotoxin testing. The system can be equipped with up to two Sunrise microplate...

Are there any consumables that need to be purchased to run PyroTec™ PRO Robotic Solution other than standard reagents, dilution tubes, and microplates?

Yes, specific pipette tips (P/N 0000229884) and troughs (P/N 0000229888) for the robotic system are purchased from Lonza. These consumables are also tested to be free of detectable endotoxin and coa can be provided.

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