The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts.

Differentiated HSMM myotubes can be maintained in SkGM™-2 medium for approximately 2-3 weeks post differentiation with media changes every other day. Once the cells have differentiated, they should be used in the culture flasks or dishes into which they have been seeded for differentiation. These cultures are post-mitotic and they will not trypsinize and replate well. Terminally differentiated cells stripped off the culture surface then cryopreserved most likely will result in massive cell death and/or poor cell adherence upon thaw.