Skeletal Muscle Cells, (SkMC) human


Cell Type:
Skeletal muscle cell
Tissue Origin:
skeletal muscle
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

 SkGM was developed for the expansion and differentiation of myoblasts into skeletal muscle cells.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P4 DS-113 1e5 55% 80% Plasmid (general) 400 ng 20 µl Shuttle

Citations (19)

Categories:
Primary Cells and Media, Transfection 
Authors:
Sato M, Dehvari N, Oberg AI, Dallner OS, Sandström AL, Olsen JM, Csikasz RI, Summers RJ, Hutchinson DS, Bengtsson T 
In:
Diabetes (2014) Epub: ahead 
Categories:
Primary Cells and Media 
Authors:
Nowis D, Malenda A, Furs K, Oleszczak B, Sadowski R, Chlebowska J, Firczuk M, Bujnicki JM, Staruch AD, Zagozdzon R, Glodkowska-Mrowka E, Szablewski L, Golab J 
In:
Other (2014) 2(1): ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Di Camillo B, Eduati F, Nair SK, Avogaro A, Toffolo GM1. 
In:
BMC Cell Biol (2014) 15: 9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fornaro M, Hinken AC, Needle S, Hu E, Trendelenburg AU, Mayer A, Rosenstiel A, Chang C, Meier V, Billin AN, Becherer JD, Brace AD, Evans WJ, Glass DJ, Russell AJ. 
In:
Am J Physiol Cell Physiol (2014) 306(2): E150-6 
Categories:
Primary Cells and Media 
Authors:
Owens J1, Moreira K, Bain G. 
In:
In Vitro Cell Dev Biol Anim. (2013) 49(9): 695-705 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shigemoto T1, Kuroda Y, Wakao S, Dezawa M 
In:
STEM CELLS TRANSLATIONALMEDICINE (2013) 2(7): 488-98 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wen MS, Wang CY, Lin SL, Hung KC. 
In:
PLoS ONE (2013) 8(5): e64025 
Categories:
Primary Cells and Media 
Authors:
Suzuki M, Honda K, Fukazawa M, Ozawa K, Hagita H, Kawai T, Takeda M, Yata T, Kawai M, Fukuzawa T, Kobayashi T, Sato T, Kawabe Y, Ikeda S. 
In:
J Pharmacol Exp Ther (2012) 341(1): 692-701 
Categories:
Primary Cells and Media 
Authors:
Straub SV, Perez SM, Tan B, Coughlan KA, Trebino CE, Cosgrove P, Buxton JM, Kreeger JM, Jackson VM 
In:
Am J Physiol Endocrinol Metab (2011) 301(2): E380-90 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chung JH, Im EK, Jin TW, Lee SM, Kim SH, Choi EY, Shin MJ, Lee KH, Jang Y 
In:
Other (2011) 43(4): 179-88 
Categories:
Primary Cells and Media 
Authors:
Mammen AL, Casciola-Rosen LA, Hall JC, Christopher-Stine L, Corse AM, Rosen A. 
In:
Arthritis Rheum (2009) 60(12): 3784-93 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Canner JA, Sobo M, Ball S, Hutzen B, DeAngelis S, Willis W, Studebaker AW, Ding K, Wang S, Yang D, Lin J 
In:
Br J Cancer (2009) 101(5): 774-81 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Trendelenburg AU, Meyer A, Rohner D, Boyle J, Hatakeyama S, Glass DJ. 
In:
Am J Physiol Cell Physiol (2009) 296(6): C1258-70 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kubo M, Egashira K, Inoue T, Koga J, Oda S, Chen L, Nakano K, Matoba T, Kawashima Y, Hara K, Tsujimoto H, Sueishi K, Tominaga R, Sunagawa K. 
In:
Arterioscler Thromb Vasc Biol (2009) 29(6): 796-801 
Categories:
Primary Cells and Media 
Authors:
Grozovsky R, Ribich S, Rosene ML, Mulcahey MA, Huang SA, Patti ME, Bianco AC, Kim BW. 
In:
Endocrinology (2009) 150(4): 1976-83 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Garcia R, Grindlay J, Rath O, Fee F, Kolch W. 
In:
EMBO Rep (2009) 10(3): 278-84 
Categories:
Primary Cells and Media 
Authors:
Taniguchi E, Nishijo K, McCleish AT, Michalek JE, Grayson MH, Infante AJ, Abboud HE, Legallo RD, Qualman SJ, Rubin BP, Keller C 
In:
Oncogene (2008) 27(51): 6550-60 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Linardic CM, Counter CM 
In:
Methods Enzymol (2008) 438: 419-427 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Su Y, Vanderlaag K, Ireland C, Ortiz J, Grage H, Safe S, Frankel AE. 
In:
Breast Cancer Res (2007) 9(4): R56 
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