Q:

Does it matter if the PBS used for monocyte enrichment contains calcium and magnesium, if the cells should be used later in Nucleofection®?
A:

The PBS should be calcium and magnesium free to prevent clumping of cells.

We routinely use PBS without calcium and magnesium for example Lonza Cat. No. BEBP17-516Q
Categories:
Transfection
Research Areas:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Basic Research

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