Q:

Can I use the ViaLight™ Plus Assay also, if my cells are cultured in 24well plates, 6well plates or 12 well plates?

A:

To do the VialIght™ Plus Assay on larger volume tissue culture ( eg 6 well , 24 well) add the cell lysis reagent equal to half the volume in the plate ( ie 100 µl culture use 50 µl cell lysis). Wait 10 minutes for the lysis . Then follow the protocol and transfer 100 µl of cell lysate into a luminescent compatible plate, add 100 µl AMR Plus, incubate for 2 minutes and then measure.

Categories:
Bioassay

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