Plasmodium yoelii yoelii 17XNL constitutively expressing GFP throughout the life cycle

Ono T, Tadakuma T, Rodriguez A
Source: Exp Parasitol
Publication Date: (2007)
Issue: 115(3): 310-3
Research Area:
Cells used in publication:
Plasmodium berghei
Species: unicellular
Tissue Origin:
Plasmodium yoelii
Species: unicellular
Tissue Origin: blood
Plasmodium yoelii is a rodent parasite commonly used as a model to study malaria infection. It is the preferred model parasite for liver-stage immunological studies and is also widely used to study hepatocyte, erythrocyte and mosquito infection. We have generated a P. yoelii yoelii 17XNL line that is stably transfected with the green fluorescent protein (gfp) gene. This parasite line constitutively expresses high levels of GFP during the complete parasite life cycle including liver, blood and mosquito stages. These fluorescent parasites can be used in combination with fluorescence activated cell sorting or live microscopy for a wide range of experimental applications.