cells

Isolated from the blood of shiny thicket rats from the Central African Republic, from Brazzaville and from Western Nigeria, it is commonly used as a malaria model organism in mice and rats, where it shows a preference for immature red blood cells.

Cell Type:

Plasmodium

Tissue Origin:

blood

Species:

unicellular

Research Area:

Immunotherapy / Hematology

Parasitology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	T cell, human	U-033				Plasmid (general)	5 µg	100 µl	I/II/2b
	T cell, human	U-033	1e7	100-1000 times higher than other electroporation methods	40-60%	cDNA	5 µg	100 µl	I/II/2b

Citations (7)

The CRISPR/Cas9 system sheds new lights on the biology of protozoan parasites.

Categories:

Transfection

Authors:

Grzybek M1, Golonko A2, Górska A3, Szczepaniak K3, Strachecka A4, Lass A5, Lisowski P6,7.

In:

Biotechnology Journal (2018) 102: 11

Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection

Categories:

Transfection

Authors:

Aly AS, Mikolajczak SA, Rivera HS, Camargo N, Jacobs-Lorena V, Labaied M, Coppens I, Kappe SH

In:

Mol Microbiol (2008) 69(1): 152-63

Plasmodium yoelii sporozoites with simultaneous deletion of P52 and P36 are completely attenuated and confer sterile immunity against infection

Categories:

Transfection

Authors:

Labaied M, Harupa A, Dumpit RF, Coppens I, Mikolajczak SA, Kappe SH

In:

Infect Immun (2007) 75(8): 3758-68

Plasmodium yoelii yoelii 17XNL constitutively expressing GFP throughout the life cycle

Categories:

Transfection

Authors:

Ono T, Tadakuma T, Rodriguez A

In:

Exp Parasitol (2007) 115(3): 310-3

Improved transfection and new selectable markers for the rodent malaria parasite Plasmodium yoelii

Categories:

Transfection

Authors:

Jongco AM, Ting LM, Thathy V, Mota MM, Kim K

In:

Mol Biochem Parasitol (2006) 146(2): 242-250

High efficiency transfection of Plasmodium berghei facilitates novel selection procedures

Categories:

Transfection

Authors:

Janse CJ, Franke-Fayard B, Mair GR, Ramesar J, Thiel C, Engelmann S, Matuschewski K, Gemert GJ, Sauerwein RW and Waters AP

In:

Mol Biochem Parasitol (2006) 145(1): 60-70

A NIMA-related Protein Kinase Is Essential for Completion of the Sexual Cycle of Malaria Parasites

Categories:

Transfection

Authors:

Reininger L, Billker O, Tewari R, Mukhopadhyay A, Fennell C, Dorin-Semblat D, Doerig C, Goldring D, Harmse L, Ranford-Cartwright L, Packer J and Doerig C

In:

J Biol Chem (2005) 280(36): 31957-31964

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