Plasmodium yoelii

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Isolated from the blood of shiny thicket rats from the Central African Republic, from Brazzaville and from Western Nigeria, it is commonly used as a malaria model organism in mice and rats, where it shows a preference for immature red blood cells.

Cell Type:
Plasmodium
Tissue Origin:
blood
Species:
unicellular
Research Area:
Immunotherapy / Hematology
Parasitology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T cell, human U-033 Plasmid (general) 5 µg 100 µl I/II/2b
T cell, human U-033 1e7 100-1000 times higher than other electroporation methods 40-60% cDNA 5 µg 100 µl I/II/2b

Citations (7)

The CRISPR/Cas9 system sheds new lights on the biology of protozoan parasites.
Categories:
Transfection 
Authors:
Grzybek M1, Golonko A2, Górska A3, Szczepaniak K3, Strachecka A4, Lass A5, Lisowski P6,7. 
In:
Biotechnology Journal (2018) 102: 11 
Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
Categories:
Transfection 
Authors:
Aly AS, Mikolajczak SA, Rivera HS, Camargo N, Jacobs-Lorena V, Labaied M, Coppens I, Kappe SH 
In:
Mol Microbiol (2008) 69(1): 152-63 
Plasmodium yoelii sporozoites with simultaneous deletion of P52 and P36 are completely attenuated and confer sterile immunity against infection
Categories:
Transfection 
Authors:
Labaied M, Harupa A, Dumpit RF, Coppens I, Mikolajczak SA, Kappe SH 
In:
Infect Immun (2007) 75(8): 3758-68 
Plasmodium yoelii yoelii 17XNL constitutively expressing GFP throughout the life cycle
Categories:
Transfection 
Authors:
Ono T, Tadakuma T, Rodriguez A 
In:
Exp Parasitol (2007) 115(3): 310-3 
Improved transfection and new selectable markers for the rodent malaria parasite Plasmodium yoelii
Categories:
Transfection 
Authors:
Jongco AM, Ting LM, Thathy V, Mota MM, Kim K 
In:
Mol Biochem Parasitol (2006) 146(2): 242-250 
High efficiency transfection of Plasmodium berghei facilitates novel selection procedures
Categories:
Transfection 
Authors:
Janse CJ, Franke-Fayard B, Mair GR, Ramesar J, Thiel C, Engelmann S, Matuschewski K, Gemert GJ, Sauerwein RW and Waters AP 
In:
Mol Biochem Parasitol (2006) 145(1): 60-70 
A NIMA-related Protein Kinase Is Essential for Completion of the Sexual Cycle of Malaria Parasites
Categories:
Transfection 
Authors:
Reininger L, Billker O, Tewari R, Mukhopadhyay A, Fennell C, Dorin-Semblat D, Doerig C, Goldring D, Harmse L, Ranford-Cartwright L, Packer J and Doerig C 
In:
J Biol Chem (2005) 280(36): 31957-31964 
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