Plasmodium berghei

One of four malaria parasites that infect murine rodents from Central Africa. It serves as a good model for the human parasites with which it shares high homology in most essential aspects of structure, biochemistry and life cycle.
Cell Type:
Plasmodium
Tissue Origin:
Species:
unicellular
Research Area:
Parasitology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T cell, human U-033 1e7 Plasmid (general) 1 µg 100 µl I/II/2b
T cell, human U-033 2e7 10^4 times higher than electroporation Plasmid (general) 5 µg 100 µl I/II/2b
T cell, human U-033 1e6 .1-1% Plasmid (general) 5 µg 100 µl I/II/2b
P5

FP-167

1e6 .1-1%


Plasmid (general) 1 µg 4D X-Unit

Citations (16)

Categories:
Transfection 
Authors:
Burda PC, Roelli MA, Schaffner M, Khan SM, Janse CJ, Heussler VT 
In:
PLoS Pathog (2015) 11(3): e1004760 
Categories:
Transfection 
Authors:
van Ooij C, Tamez P, Bhattacharjee S, Hiller NL, Harrison T, Liolios K, Kooij T, Ramesar J, Balu B, Adams J, Waters A, Janse C, Haldar K 
In:
PLoS Pathog (2008) 4(6): e1000084 
Categories:
Transfection 
Authors:
Silvie O, Goetz K, Matuschewski K 
In:
PLoS Pathog (2008) 4(6): e1000086 
Categories:
Transfection 
Authors:
MacKenzie JJ, Gomez ND, Bhattacharjee S, Mann S, Haldar K 
In:
PLoS ONE (2008) 3(6): e2405 
Categories:
Transfection 
Authors:
Buchholz K, Rahlfs S, Schirmer RH, Becker K, Matuschewski K 
In:
PLoS ONE (2008) 3(6): e2474 
Categories:
Transfection 
Authors:
Ellekvist P, Maciel J, Mlambo G, Ricke CH, Colding H, Klaerke DA, Kumar N 
In:
Proc Natl Acad Sci USA (2008) 105(17): 6398-402 
Categories:
Transfection 
Authors:
Ecker A, Pinto SB, Baker KW, Kafatos FC, Sinden RE 
In:
Exp Parasitol (2007) 116(4): 504-8 
Categories:
Transfection 
Authors:
Raine JD, Ecker A, Mendoza J, Tewari R, Stanway RR, Sinden RE 
In:
PLoS Pathog (2007) 3(3): e30 
Categories:
Transfection 
Authors:
Ono T, Tadakuma T, Rodriguez A 
In:
Exp Parasitol (2007) 115(3): 310-3 
Categories:
Transfection 
Authors:
Janse CJ, Ramesar J, Waters AP 
In:
Nat Protocols (2006) 1(1): 346-356 
Categories:
Transfection 
Authors:
Janse CJ, Franke-Fayard B, Waters AP 
In:
Nat Protocols (2006) 1(2): 614-623 
Categories:
Transfection 
Authors:
Jongco AM, Ting LM, Thathy V, Mota MM, Kim K 
In:
Mol Biochem Parasitol (2006) 146(2): 242-250 
Categories:
Transfection 
Authors:
Janse CJ, Franke-Fayard B, Mair GR, Ramesar J, Thiel C, Engelmann S, Matuschewski K, Gemert GJ, Sauerwein RW and Waters AP 
In:
Mol Biochem Parasitol (2006) 145(1): 60-70 
Categories:
Transfection 
Authors:
Sakamoto H, Thiberge S, Akerman S, Janse CJ, Carvalho TG and Menard R 
In:
Nucleic Acids Res (2005) 33(20): e174 
Categories:
Transfection 
Authors:
Reininger L, Billker O, Tewari R, Mukhopadhyay A, Fennell C, Dorin-Semblat D, Doerig C, Goldring D, Harmse L, Ranford-Cartwright L, Packer J and Doerig C 
In:
J Biol Chem (2005) 280(36): 31957-31964 
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