cells

One of four malaria parasites that infect murine rodents from Central Africa. It serves as a good model for the human parasites with which it shares high homology in most essential aspects of structure, biochemistry and life cycle.

Cell Type:

Plasmodium

Tissue Origin:

Species:

unicellular

Research Area:

Parasitology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Substrate		Format	Platform
T cell, human	U-033	1e7		Plasmid (general)	1 µg	100 µl	I/II/2b
T cell, human	U-033	2e7	10^4 times higher than electroporation	Plasmid (general)	5 µg	100 µl	I/II/2b
T cell, human	U-033	1e6	.1-1%	Plasmid (general)	5 µg	100 µl	I/II/2b
P5	FP-167	1e6	.1-1%	Plasmid (general)	1 µg		4D X-Unit

Citations (17)

Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes

Categories:

Transfection

Authors:

Bushell E, Gomes AR, Sanderson T, Anar B, Girling G, Herd C, Metcalf T, Modrzynska K, Schwach F, Martin RE, Mather MW, McFadden GI, Parts L, Rutledge GG, Vaidya AB, Wengelnik K, Rayner JC, Billker O.

In:

Cell (2017) 170(2): 260-272.e8.

A Plasmodium phospholipase is involved in disruption of the liver stage parasitophorous vacuole membrane.

Categories:

Transfection

Authors:

Burda PC, Roelli MA, Schaffner M, Khan SM, Janse CJ, Heussler VT

In:

PLoS Pathog (2015) 11(3): e1004760

The malaria secretome: from algorithms to essential function in blood stage infection

Categories:

Transfection

Authors:

van Ooij C, Tamez P, Bhattacharjee S, Hiller NL, Harrison T, Liolios K, Kooij T, Ramesar J, Balu B, Adams J, Waters A, Janse C, Haldar K

In:

PLoS Pathog (2008) 4(6): e1000084

A sporozoite asparagine-rich protein controls initiation of Plasmodium liver stage development

Categories:

Transfection

Authors:

Silvie O, Goetz K, Matuschewski K

In:

PLoS Pathog (2008) 4(6): e1000086

A Plasmodium falciparum host-targeting motif functions in export during blood stage infection of the rodent malarial parasite Plasmodium berghei

Categories:

Transfection

Authors:

MacKenzie JJ, Gomez ND, Bhattacharjee S, Mann S, Haldar K

In:

PLoS ONE (2008) 3(6): e2405

Depletion of Plasmodium berghei plasmoredoxin reveals a non-essential role for life cycle progression of the malaria parasite

Categories:

Transfection

Authors:

Buchholz K, Rahlfs S, Schirmer RH, Becker K, Matuschewski K

In:

PLoS ONE (2008) 3(6): e2474

A Murine Model for Assessment of Plasmodium falciparum Transmission Blocking Vaccine using Transgenic Plasmodium berghei Parasites Expressing the target antigen Pfs25

Categories:

Transfection

Authors:

Mlambo G, Maciel J, Kumar N

In:

Infect Immun (2008) 76(5): 2018-24

Critical role of a K+ channel in Plasmodium berghei transmission revealed by targeted gene disruption

Categories:

Transfection

Authors:

Ellekvist P, Maciel J, Mlambo G, Ricke CH, Colding H, Klaerke DA, Kumar N

In:

Proc Natl Acad Sci USA (2008) 105(17): 6398-402

Plasmodium berghei: plasmodium perforin-like protein 5 is required for mosquito midgut invasion in Anopheles stephensi

Categories:

Transfection

Authors:

Ecker A, Pinto SB, Baker KW, Kafatos FC, Sinden RE

In:

Exp Parasitol (2007) 116(4): 504-8

Female Inheritance of Malarial lap Genes Is Essential for Mosquito Transmission

Categories:

Transfection

Authors:

Raine JD, Ecker A, Mendoza J, Tewari R, Stanway RR, Sinden RE

In:

PLoS Pathog (2007) 3(3): e30

Plasmodium yoelii yoelii 17XNL constitutively expressing GFP throughout the life cycle

Categories:

Transfection

Authors:

Ono T, Tadakuma T, Rodriguez A

In:

Exp Parasitol (2007) 115(3): 310-3

High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei

Categories:

Transfection

Authors:

Janse CJ, Ramesar J, Waters AP

In:

Nat Protocols (2006) 1(1): 346-356

Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium berghei

Categories:

Transfection

Authors:

Janse CJ, Franke-Fayard B, Waters AP

In:

Nat Protocols (2006) 1(2): 614-623

Improved transfection and new selectable markers for the rodent malaria parasite Plasmodium yoelii

Categories:

Transfection

Authors:

Jongco AM, Ting LM, Thathy V, Mota MM, Kim K

In:

Mol Biochem Parasitol (2006) 146(2): 242-250

High efficiency transfection of Plasmodium berghei facilitates novel selection procedures

Categories:

Transfection

Authors:

Janse CJ, Franke-Fayard B, Mair GR, Ramesar J, Thiel C, Engelmann S, Matuschewski K, Gemert GJ, Sauerwein RW and Waters AP

In:

Mol Biochem Parasitol (2006) 145(1): 60-70

Towards systematic identification of Plasmodium essential genes by transposon shuttle mutagenesis

Categories:

Transfection

Authors:

Sakamoto H, Thiberge S, Akerman S, Janse CJ, Carvalho TG and Menard R

In:

Nucleic Acids Res (2005) 33(20): e174

A NIMA-related Protein Kinase Is Essential for Completion of the Sexual Cycle of Malaria Parasites

Categories:

Transfection

Authors:

Reininger L, Billker O, Tewari R, Mukhopadhyay A, Fennell C, Dorin-Semblat D, Doerig C, Goldring D, Harmse L, Ranford-Cartwright L, Packer J and Doerig C

In:

J Biol Chem (2005) 280(36): 31957-31964

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