Jurkat-modified

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having an integrated transgene

Cell Type:
T Cell
Tissue Origin:
Species:
human
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V I-010 2e6 37% 83% Plasmid (general) 2 µg 100 µl I/II/2b
V I-010 2e6 70% 75% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (40)

Naturally occurring T cell mutations enhance engineered T cell therapies
Categories:
Serum-free and Speciality Media, Transfection 
Authors:
Garcia J, Daniels J, Lee Y, Zhu I, Cheng K, Liu Q, Goodman D, Burnett C, Law C, Thienpont C, Alavi J, Azimi C, Montgomery G, Roybal KT, Choi J 
In:
Nature (2024) 626(7999): 626-634 
High-throughput T cell receptor engineering by functional screening identifies candidates with enhanced potency and specificity
Categories:
Transfection 
Authors:
Rodrigo Vazquez-Lombardi, Johanna S. Jung, Fabrice S. Schlatter, Anna Mei, Natalia Rodrigues Mantuano, Florian Bieberich, Kai-Lin Hong, Jakub Kucharczyk, Edo Kapetanovic, Erik Aznauryan, Cedric R. Weber, Alfred Zippelius, Heinz Laeubli, and Sai T. Reddy 
In:
Immunity (2022) 55: 1953-1966 
No advantage of cell-penetrating peptides over receptor-specific antibodies in targeting antigen to human dendritic cells for cross-presentation
Categories:
Transfection 
Authors:
Tacken PJ, Joosten B, Reddy A, Wu D, Eek A, Laverman P, Kretz-Rommel A, Adema GJ, Torensma R, Figdor CG 
In:
J Immunol (2008) 180(11): 7687-96 
CEACAM1 dynamics during neisseria gonorrhoeae suppression of CD4+ T lymphocyte activation
Categories:
Transfection 
Authors:
Lee HS, Ostrowski MA, Gray-Owen SD 
In:
J Immunol (2008) 180(10): 6827-35 
Chondrogenic differentiation of human bone marrow stem cells in transwell cultures: generation of scaffold-free cartilage.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Murdoch AD, Grady LM, Ablett MP, Katopodi T, Meadows RS, Hardingham TE 
In:
Stem Cells (2007) 25(11): 2786-96 
Signal-Transducing Adaptor Protein-2 Regulates Integrin-Mediated T Cell Adhesion through Protein Degradation of Focal Adhesion Kinase
Categories:
Transfection 
Authors:
Sekine Y, Tsuji S, Ikeda O, Sugiyma K, Oritani K, Shimoda K, Muromoto R, Ohbayashi N, Yoshimura A, Matsuda T 
In:
J Immunol (2007) 179(4): 2397-407 
The lymphocyte function-associated antigen-1 receptor costimulates plasma membrane Ras via phospholipase D2
Categories:
Transfection 
Authors:
Mor A, Campi G, Du G, Zheng Y, Foster DA, Dustin ML, Philips MR 
In:
Nat Cell Biol (2007) 9(6): 713-9 
Caveolin-1 triggers T-cell activation via CD26 in association with CARMA1
Categories:
Transfection 
Authors:
Ohnuma K, Uchiyama M, Yamochi T, Nishibashi K, Hosono O, Takahashi N, Kina S, Tanaka H, Lin X, Dang NH, Morimoto C 
In:
J Biol Chem (2007) 282(13): 10117-31 
A functional SNP of interferon- gene is important for interferon--induced and spontaneous recovery from hepatitis C virus infection
Categories:
Transfection 
Authors:
Huang Y, Yang H, Borg BB, Su X, Rhodes SL, Yang K, Tong X, Tang G, Howell CD, Rosen HR, Thio CL, Thomas DL, Alter HJ, Sapp RK, Liang TJ 
In:
Proc Natl Acad Sci USA (2007) 104(3): 985-90 
The marine product cephalostatin 1 activates an ER stress-specific and apoptosome-independent apoptotic signaling pathway
Categories:
Transfection 
Authors:
Lopez-Anton N, Rudy A, Barth N, Schmitz ML, Pettit GR, Schulze-Osthoff K, Dirsch VM, Vollmar AM 
In:
J Biol Chem (2006) 281(44): 33078-86 
CD28 interaction with filamin-A controls lipid raft accumulation at the T-cell immunological synapse
Categories:
Transfection 
Authors:
Tavano R, Contento RL, Baranda SJ, Soligo M, Tuosto L, Manes S, Viola A 
In:
Nat Cell Biol (2006) 8(11): 1270-6 
A Single Polymorphism Disrupts the Killer Ig-Like Receptor 2DL2/2DL3 D1 Domain
Categories:
Transfection 
Authors:
Vandenbussche CJ, Dakshanamurthy S, Posch PE, Hurley CK 
In:
J Immunol (2006) 177(8): 5347-57 
The TRAF3-binding site of human molluscipox virus FLIP molecule MC159 is critical for its capacity to inhibit Fas-induced apoptosis
Categories:
Transfection 
Authors:
Thurau M, Everett H, Tapernoux M, Tschopp J, Thome M 
In:
Cell Death Differ (2006) 13(9): 1577-85 
9-tetrahydrocannabinol-induced apoptosis in jurkat leukemia T cells is regulated by translocation of bad to mitochondria
Categories:
Transfection 
Authors:
Jia W, Hegde VL, Singh NP, Sisco D, Grant S, Nagarkatti M, Nagarkatti PS 
In:
Mol Cancer Res (2006) 4(8): 549-62 
The SWI/SNF chromatin-remodeling complex is a cofactor for tat transactivation of the HIV promoter
Categories:
Transfection 
Authors:
Mahmoudi T, Parra M, Vries RG, Kauder SE, Verrijzer CP, Ott M, Verdin E 
In:
J Biol Chem (2006) 281(29): 19960-8 
Identification of an Intracellular Trafficking and Assembly Pathway for HIV-1 Gag
Categories:
Transfection 
Authors:
Perlman M, Resh MD 
In:
Traffic (2006) 7(6): 731-45 
Protein Kinase C (PKC) and PKC Are the Major PKC Isotypes Involved in TCR Down-Regulation
Categories:
Transfection 
Authors:
von Essen M, Nielsen MW, Bonefeld CM, Boding L, Larsen JM, Leitges M, Baier G, Odum N, Geisler C 
In:
J Immunol (2006) 176(12): 7502-10 
HTLV-1 tax protects against CD95-mediated apoptosis by induction of the cellular FLICE-inhibitory protein (c-FLIP)
Categories:
Transfection 
Authors:
Krueger A, Fas SC, Giaisi M, Bleumink M, Merling A, Stumpf C, Baumann S, Holtkotte D, Bosch V, Krammer PH and Li-Weber M 
In:
Blood (2006) 107(10): 3933-9 
HIV-1 GP120-mediated apoptosis of T cells is regulated by the membrane tyrosine phosphatase, CD45
Categories:
Transfection 
Authors:
Anand AR, Ganju RK 
In:
J Biol Chem (2006) 281(18): 12289-99 
Transcriptional repression of human immunodeficiency virus type 1 by AP-4
Categories:
Transfection 
Authors:
Imai K, Okamoto T 
In:
J Biol Chem (2006) 281(18): 12495-505 
Loss of Siglec expression on T lymphocytes during human evolution
Categories:
Transfection 
Authors:
Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A 
In:
Proc Natl Acad Sci USA (2006) 103(20): 7765-70 
Long-term engagement of CD6 and ALCAM is essential for T cell proliferation induced by dendritic cells
Categories:
Transfection 
Authors:
Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN and Figdor CG 
In:
Blood (2006) 107(8): 3212-20 
A Novel NF-B Binding Site Controls Human Granzyme B Gene Transcription
Categories:
Transfection 
Authors:
Huang C, Bi E, Hu Y, Deng W, Tian Z, Dong C, Hu Y, Sun B 
In:
J Immunol (2006) 176(7): 4173-81 
The adaptor protein Nck interacts with Fas ligand: Guiding the death factor to the cytotoxic immunological synapse
Categories:
Transfection 
Authors:
Lettau M, Qian J, Linkermann A, Latreille M, Larose L, Kabelitz D, Janssen O 
In:
Proc Natl Acad Sci USA (2006) 103(15): 5911-6 
Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane
Categories:
Transfection 
Authors:
Booth AM, Fang Y, Fallon JK, Yang JM, Hildreth JE, Gould SJ 
In:
J Cell Biol (2006) 172(6): 923-35 
The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells
Categories:
Transfection 
Authors:
Weinberg MS, Villeneuve LM, Ehsani A, Amarzguioui M, Aagaard L, Chen ZX, Riggs AD, Rossi JJ and Morris KV 
In:
RNA (2006) 12(2): 256-262 
The human CD94 gene encodes multiple, expressible transcripts including a new partner of NKG2A/B
Categories:
Transfection 
Authors:
Lieto LD, Maasho K, West D, Borrego F and Coligan JE 
In:
Genes Immun (2006) 7(1): 36-43 
Role for the Abi/wave protein complex in T cell receptor-mediated proliferation and cytoskeletal remodeling
Categories:
Transfection 
Authors:
Zipfel PA, Bunnell SC, Witherow DS, Gu JJ, Chislock EM, Ring C, Pendergast AM 
In:
Curr Biol (2006) 16(1): 35-46 
Human T-lymphotropic virus, type 1, tax protein triggers microtubule reorientation in the virological synapse
Categories:
Transfection 
Authors:
Nejmeddine M, Barnard AL, Tanaka Y, Taylor GP and Bangham CR 
In:
J Biol Chem (2005) 280(33): 29653-29660 
CUL1, a component of E3 ubiquitin ligase, alters lymphocyte signal transduction with possible effect on rheumatoid arthritis
Categories:
Transfection 
Authors:
Kawaida R, Yamada R, Kobayashi K, Tokuhiro S, Suzuki A, Kochi Y, Chang X, Sekine A, Tsunoda T, Sawada T, Furukawa H, Nakamura Y and Yamamoto K 
In:
Genes Immun (2005) 6(3): 194-202 
Identification of proteoglycans as the APRIL-specific binding partners
Categories:
Transfection 
Authors:
Ingold K, Zumsteg A, Tardivel A, Huard B, Steiner QG, Cachero TG, Qiang F, Gorelik L, Kalled SL, Acha-Orbea H, Rennert PD, Tschopp J, Schneider P 
In:
J Exp Med (2005) 201(9): 1375-1383 
EBV-Induced Gene 3 Transcription Is Induced by TLR Signaling in Primary Dendritic Cells via NF-(kappa)B Activation
Categories:
Transfection 
Authors:
Wirtz S, Becker C, Fantini MC, Nieuwenhuis EE, Tubbe I, Galle PR, Schild HJ, Birkenbach M, Blumberg RS and Neurath MF 
In:
J Immunol (2005) 174(5): 2814-2824 
Triterpenoid electrophiles (avicins) suppress heat shock protein-70 and x-linked inhibitor of apoptosis proteins in malignant cells by activation of ubiquitin machinery: implications for proapoptotic activity
Categories:
Transfection 
Authors:
Gaikwad A, Poblenz A, Haridas V, Zhang C, Duvic M and Gutterman J 
In:
Clin Cancer Res (2005) 11(5): 1953-1962 
Human Fas-associated factor 1, interacting with ubiquitinated proteins and valosin-containing protein, is involved in the ubiquitin-proteasome pathway
Categories:
Transfection 
Authors:
Song EJ, Yim SH, Kim E, Kim NS and Lee KJ 
In:
Mol Cell Biol (2005) 25(6): 2511-2524 
Decrease of Lewis frequency in HIV-infected patients: possible competition of fucosylated antigens with HIV for binding to DC-SIGN
Categories:
Transfection 
Authors:
Puissant B, Roubinet F, Dellacasagrande J, Massip P, Abbal M, Pasquier C, Izopet J, Blancher A 
In:
AIDS (2005) 19(6): 627-30 
Unexpected side chain effects at residue 8 of cyclosporin a derivatives allow photoswitching of immunosuppression
Categories:
Transfection 
Authors:
Zhang Y, Erdmann F, Baumgrass R, Schutkowski M and Fischer G 
In:
J Biol Chem (2005) 280(6): 4842-4850 
Kinase-Independent Functions for Itk in TCR-Induced Regulation of Vav and the Actin Cytoskeleton
Categories:
Transfection 
Authors:
Dombroski D, Houghtling RA, Labno CM, Precht P, Takesono A, Caplen NJ, Billadeau DD, Wange RL, Burkhardt JK and Schwartzberg PL 
In:
J Immunol (2005) 174(3): 1385-1392 
PTEN regulates motility but not directionality during leukocyte chemotaxis
Categories:
Transfection 
Authors:
Lacalle RA, Gomez-Mouton C, Barber DF, Jimenez-Baranda S, Mira E, Martinez-A C, Carrera AC, Manes S 
In:
J Cell Sci (2004) 117(Pt 25): 6207-6215 
Nicotine Activates Nuclear Factor of Activated T Cells c2 (NFATc2) and Prevents Cell Cycle Entry in T Cells
Categories:
Transfection 
Authors:
Frazer-Abel AA, Baksh S, Fosmire SP, Willis D, Pierce AM, Meylemans H, Linthicum DS, Burakoff SJ, Coons T, Bellgrau D and Modiano JF 
In:
J Pharmacol Exp Ther (2004) 311(2): 758-769 
Mechanism of prostaglandin (PG)E2-induced prolactin expression in human T cells: cooperation of two PGE2 receptor subtypes, E-prostanoid (EP) 3 and EP4, via calcium- and cyclic adenosine 5'-monophosphate-mediated signaling pathways
Categories:
Transfection 
Authors:
Gerlo S, Verdood P, Gellersen B, Hooghe-Peters EL and Kooijman R 
In:
J Immunol (2004) 173(10): 5952-5962 
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