Jurkat-modified

having an integrated transgene

Cell Type:
T Cell
Tissue Origin:
Species:
human
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V I-010 2e6 37% 83% Plasmid (general) 2 µg 100 µl I/II/2b
V I-010 2e6 70% 75% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (38)

Categories:
Transfection 
Authors:
Tacken PJ, Joosten B, Reddy A, Wu D, Eek A, Laverman P, Kretz-Rommel A, Adema GJ, Torensma R, Figdor CG 
In:
J Immunol (2008) 180(11): 7687-96 
Categories:
Transfection 
Authors:
Lee HS, Ostrowski MA, Gray-Owen SD 
In:
J Immunol (2008) 180(10): 6827-35 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Murdoch AD, Grady LM, Ablett MP, Katopodi T, Meadows RS, Hardingham TE 
In:
Stem Cells (2007) 25(11): 2786-96 
Categories:
Transfection 
Authors:
Sekine Y, Tsuji S, Ikeda O, Sugiyma K, Oritani K, Shimoda K, Muromoto R, Ohbayashi N, Yoshimura A, Matsuda T 
In:
J Immunol (2007) 179(4): 2397-407 
Categories:
Transfection 
Authors:
Mor A, Campi G, Du G, Zheng Y, Foster DA, Dustin ML, Philips MR 
In:
Nat Cell Biol (2007) 9(6): 713-9 
Categories:
Transfection 
Authors:
Ohnuma K, Uchiyama M, Yamochi T, Nishibashi K, Hosono O, Takahashi N, Kina S, Tanaka H, Lin X, Dang NH, Morimoto C 
In:
J Biol Chem (2007) 282(13): 10117-31 
Categories:
Transfection 
Authors:
Huang Y, Yang H, Borg BB, Su X, Rhodes SL, Yang K, Tong X, Tang G, Howell CD, Rosen HR, Thio CL, Thomas DL, Alter HJ, Sapp RK, Liang TJ 
In:
Proc Natl Acad Sci USA (2007) 104(3): 985-90 
Categories:
Transfection 
Authors:
Lopez-Anton N, Rudy A, Barth N, Schmitz ML, Pettit GR, Schulze-Osthoff K, Dirsch VM, Vollmar AM 
In:
J Biol Chem (2006) 281(44): 33078-86 
Categories:
Transfection 
Authors:
Tavano R, Contento RL, Baranda SJ, Soligo M, Tuosto L, Manes S, Viola A 
In:
Nat Cell Biol (2006) 8(11): 1270-6 
Categories:
Transfection 
Authors:
Vandenbussche CJ, Dakshanamurthy S, Posch PE, Hurley CK 
In:
J Immunol (2006) 177(8): 5347-57 
Categories:
Transfection 
Authors:
Thurau M, Everett H, Tapernoux M, Tschopp J, Thome M 
In:
Cell Death Differ (2006) 13(9): 1577-85 
Categories:
Transfection 
Authors:
Jia W, Hegde VL, Singh NP, Sisco D, Grant S, Nagarkatti M, Nagarkatti PS 
In:
Mol Cancer Res (2006) 4(8): 549-62 
Categories:
Transfection 
Authors:
Mahmoudi T, Parra M, Vries RG, Kauder SE, Verrijzer CP, Ott M, Verdin E 
In:
J Biol Chem (2006) 281(29): 19960-8 
Categories:
Transfection 
Authors:
Perlman M, Resh MD 
In:
Traffic (2006) 7(6): 731-45 
Categories:
Transfection 
Authors:
von Essen M, Nielsen MW, Bonefeld CM, Boding L, Larsen JM, Leitges M, Baier G, Odum N, Geisler C 
In:
J Immunol (2006) 176(12): 7502-10 
Categories:
Transfection 
Authors:
Krueger A, Fas SC, Giaisi M, Bleumink M, Merling A, Stumpf C, Baumann S, Holtkotte D, Bosch V, Krammer PH and Li-Weber M 
In:
Blood (2006) 107(10): 3933-9 
Categories:
Transfection 
Authors:
Anand AR, Ganju RK 
In:
J Biol Chem (2006) 281(18): 12289-99 
Categories:
Transfection 
Authors:
Imai K, Okamoto T 
In:
J Biol Chem (2006) 281(18): 12495-505 
Categories:
Transfection 
Authors:
Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A 
In:
Proc Natl Acad Sci USA (2006) 103(20): 7765-70 
Categories:
Transfection 
Authors:
Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN and Figdor CG 
In:
Blood (2006) 107(8): 3212-20 
Categories:
Transfection 
Authors:
Huang C, Bi E, Hu Y, Deng W, Tian Z, Dong C, Hu Y, Sun B 
In:
J Immunol (2006) 176(7): 4173-81 
Categories:
Transfection 
Authors:
Lettau M, Qian J, Linkermann A, Latreille M, Larose L, Kabelitz D, Janssen O 
In:
Proc Natl Acad Sci USA (2006) 103(15): 5911-6 
Categories:
Transfection 
Authors:
Booth AM, Fang Y, Fallon JK, Yang JM, Hildreth JE, Gould SJ 
In:
J Cell Biol (2006) 172(6): 923-35 
Categories:
Transfection 
Authors:
Weinberg MS, Villeneuve LM, Ehsani A, Amarzguioui M, Aagaard L, Chen ZX, Riggs AD, Rossi JJ and Morris KV 
In:
RNA (2006) 12(2): 256-262 
Categories:
Transfection 
Authors:
Lieto LD, Maasho K, West D, Borrego F and Coligan JE 
In:
Genes Immun (2006) 7(1): 36-43 
Categories:
Transfection 
Authors:
Zipfel PA, Bunnell SC, Witherow DS, Gu JJ, Chislock EM, Ring C, Pendergast AM 
In:
Curr Biol (2006) 16(1): 35-46 
Categories:
Transfection 
Authors:
Nejmeddine M, Barnard AL, Tanaka Y, Taylor GP and Bangham CR 
In:
J Biol Chem (2005) 280(33): 29653-29660 
Categories:
Transfection 
Authors:
Kawaida R, Yamada R, Kobayashi K, Tokuhiro S, Suzuki A, Kochi Y, Chang X, Sekine A, Tsunoda T, Sawada T, Furukawa H, Nakamura Y and Yamamoto K 
In:
Genes Immun (2005) 6(3): 194-202 
Categories:
Transfection 
Authors:
Ingold K, Zumsteg A, Tardivel A, Huard B, Steiner QG, Cachero TG, Qiang F, Gorelik L, Kalled SL, Acha-Orbea H, Rennert PD, Tschopp J, Schneider P 
In:
J Exp Med (2005) 201(9): 1375-1383 
Categories:
Transfection 
Authors:
Wirtz S, Becker C, Fantini MC, Nieuwenhuis EE, Tubbe I, Galle PR, Schild HJ, Birkenbach M, Blumberg RS and Neurath MF 
In:
J Immunol (2005) 174(5): 2814-2824 
Categories:
Transfection 
Authors:
Gaikwad A, Poblenz A, Haridas V, Zhang C, Duvic M and Gutterman J 
In:
Clin Cancer Res (2005) 11(5): 1953-1962 
Categories:
Transfection 
Authors:
Song EJ, Yim SH, Kim E, Kim NS and Lee KJ 
In:
Mol Cell Biol (2005) 25(6): 2511-2524 
Categories:
Transfection 
Authors:
Puissant B, Roubinet F, Dellacasagrande J, Massip P, Abbal M, Pasquier C, Izopet J, Blancher A 
In:
AIDS (2005) 19(6): 627-30 
Categories:
Transfection 
Authors:
Zhang Y, Erdmann F, Baumgrass R, Schutkowski M and Fischer G 
In:
J Biol Chem (2005) 280(6): 4842-4850 
Categories:
Transfection 
Authors:
Dombroski D, Houghtling RA, Labno CM, Precht P, Takesono A, Caplen NJ, Billadeau DD, Wange RL, Burkhardt JK and Schwartzberg PL 
In:
J Immunol (2005) 174(3): 1385-1392 
Categories:
Transfection 
Authors:
Lacalle RA, Gomez-Mouton C, Barber DF, Jimenez-Baranda S, Mira E, Martinez-A C, Carrera AC, Manes S 
In:
J Cell Sci (2004) 117(Pt 25): 6207-6215 
Categories:
Transfection 
Authors:
Frazer-Abel AA, Baksh S, Fosmire SP, Willis D, Pierce AM, Meylemans H, Linthicum DS, Burakoff SJ, Coons T, Bellgrau D and Modiano JF 
In:
J Pharmacol Exp Ther (2004) 311(2): 758-769 
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