cells

human acute myeloid leukemia

Cell Type:

Leukocyte (unspec.)

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Recommended Media

Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	T-019	2e6	43% ±3	65%	Plasmid (general)	1 µg	100 µl	I/II/2b
V	T-019	2e6	50% ±1	65%	Plasmid (general)	1 µg	100 µl	I/II/2b
SF	96-EN-138	4e5	58% ±5	61% ±7	Plasmid (general)	0.4 µg	20 µl	96-well
SF	96-EN-138	4e5			siRNA	8 ng	20 µl	96-well
SF	96-EN-138	4e5			siRNA	80 ng	20 µl	96-well
SF	EN-138	4e5	44.7% ±2	61.4% ±5	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	EN-138	2e6	48.2% ±1	49.3% ±1	Plasmid (general)	2 µg	100 µl	4D X-Unit

Citations (33)

The PCH Family Member Proline-Serine-Threonine Phosphatase-interacting Protein 1 Targets to the Leukocyte Uropod and Regulates Directed Cell Migration

Categories:

Transfection

Authors:

Cooper KM, Bennin DA, Huttenlocher A

In:

Mol Biol Cell (2008) 19(8): 3180-91

Vitamin D3 induces autophagy of human myeloid leukemia cells

Categories:

Transfection

Authors:

Wang J, Lian H, Zhao Y, Kauss MA, Spindel S

In:

J Biol Chem (2008) epub: online

Munc13-4 regulates granule secretion in human neutrophils

Categories:

Transfection

Authors:

Pivot-Pajot C, Varoqueaux F, de Saint Basile G, Bourgoin SG

In:

J Immunol (2008) 180(10): 6786-6797

Mechanisms of antileukemic activity of the novel Bcl-2 homology domain-3 mimetic GX15-070 (obatoclax).

Categories:

Transfection

Authors:

Konopleva M, Watt J, Contractor R, Tsao T, Harris D, Estrov Z, Bornmann W, Kantarjian H, Viallet J, Samudio I, Andreeff M

In:

Cancer Res (2008) 68(9): 3413-20

Epigenetic modification of CCAAT/enhancer binding protein alpha expression in acute myeloid leukemia

Categories:

Transfection

Authors:

Hackanson B, Bennett KL, Brena RM, Jiang J, Claus R, Chen SS, Blagitko-Dorfs N, Maharry K, Whitman SP, Schmittgen TD, Lübbert M, Marcucci G, Bloomfield CD, Plass C

In:

Cancer Res (2008) 68(9): 3142-51

Identification of a novel p53-dependent activation pathway of STAT1 by antitumour genotoxic agents

Categories:

Transfection

Authors:

Youlyouz-Marfak I, Gachard N, Le Clorennec C, Najjar I, Baran-Marszak F, Reminieras L, May E, Bornkamm GW, Fagard R, Feuillard J

In:

Cell Death Differ (2008) 15(2): 376-85

The MDM-2 antagonist nutlin-3 promotes the maturation of acute myeloid leukemic blasts

Categories:

Transfection

Authors:

Secchiero P, Zerbinati C, Melloni E, Milani D, Campioni D, Fadda R, Tiribelli M, Zauli G

In:

Neoplasia (2007) 9(10): 853-61

Novel RUNX1 isoforms determine the fate of acute myeloid leukemia cells by controlling CD56 expression

Categories:

Transfection

Authors:

Gattenloehner S, Chuvpilo S, Langebrake C, Reinhardt D, Muller-Hermelink HK, Serfling E, Vincent A, Marx A

In:

Blood (2007) 110(6): 2027-33

Sp110 transcription is induced and required by Anaplasma phagocytophilum for infection of human promyelocytic cells

Categories:

Transfection

Authors:

de la Fuente J, Manzano-Roman R, Blouin EF, Naranjo V, Kocan KM

In:

BMC Infect Dis (2007) 7(1): 110

Cytotoxic phospholipid oxidation products: Cell death from mitochondrial damage and the intrinsic caspase cascade

Categories:

Transfection

Authors:

Chen R, Yang L, McIntyre TM

In:

J Biol Chem (2007) 282(34): 24842-50

NB1 mediates surface expression of the ANCA antigen proteinase 3 on human neutrophils

Categories:

Transfection

Authors:

von Vietinghoff S, Tunnemann G, Eulenberg C, Wellner M, Cardoso MC, Luft FC, Kettritz R

In:

Blood (2007) 109(10): 4487-93

Cross-talk between IRAK-4 and the NADPH oxidase

Categories:

Transfection

Authors:

Pacquelet S, Johnson JL, Ellis BA, Brzezinska AA, Lane WS, Munafo DB, Catz SD

In:

Biochem J (2007) 403(3): 451-61

MIF is a noncognate ligand of CXC chemokine receptors in inflammatory and atherogenic cell recruitment

Categories:

Transfection

Authors:

Bernhagen J, Krohn R, Lue H, Gregory JL, Zernecke A, Koenen RR, Dewor M, Georgiev I, Schober A, Leng L, Kooistra T, Fingerle-Rowson G, Ghezzi P, Kleemann R, McColl SR, Bucala R, Hickey MJ, Weber C

In:

Nat Med (2007) 13(5): 587-596

Honokiol Induces a Necrotic Cell Death through the Mitochondrial Permeability Transition Pore

Categories:

Transfection

Authors:

Li L, Han W, Gu Y, Qiu S, Lu Q, Jin J, Luo J, Hu X

In:

Cancer Res (2007) 67(10): 4894-903

Iron deprivation blocks multilineage haematopoietic differentiation by inhibiting induction of p21(WAF1/CIP1).

Categories:

Primary Cells and Media

Authors:

Alcantara O, Boldt DH

In:

Br J of Haematol (2007) 137(3): 252-61

Identification of molecular predictors of response in a study of tipifarnib treatment in relapsed and refractory acute myelogenous leukemia

Categories:

Transfection

Authors:

Raponi M, Harousseau JL, Lancet JE, Lowenberg B, Stone R, Zhang Y, Rackoff W, Wang Y, Atkins D

In:

Clin Cancer Res (2007) 13(7): 2254-60

Rab27a is a key component of the secretory machinery of azurophilic granules in granulocytes

Categories:

Transfection

Authors:

Munafo DB, Johnson JL, Ellis BA, Rutschmann S, Beutler B, Catz SD

In:

Biochem J (2007) 402(2): 229-39

Early growth response transcriptional regulators are dispensable for macrophage differentiation

Categories:

Transfection

Authors:

Carter JH, Tourtellotte WG

In:

J Immunol (2007) 178(5): 3038-47

Knockdown of XAB2 Enhances All-Trans Retinoic Acid-Induced Cellular Differentiation in All-Trans Retinoic Acid-Sensitive and -Resistant Cancer Cells

Categories:

Transfection

Authors:

Ohnuma-Ishikawa K, Morio T, Yamada T, Sugawara Y, Ono M, Nagasawa M, Yasuda A, Morimoto C, Ohnuma K, Dang NH, Hosoi H, Verdin E, Mizutani S

In:

Cancer Res (2007) 67(3): 1019-29

DNA repair contributes to the drug-resistant phenotype of primary acute myeloid leukaemia cells with FLT3 internal tandem duplications and is reversed by the FLT3 inhibitor PKC412

Categories:

Transfection

Authors:

Seedhouse CH, Hunter HM, Lloyd-Lewis B, Massip AM, Pallis M, Carter GI, Grundy M, Shang S, Russell NH

In:

Leukemia (2006) 20(12): 2130-2136

Histone deacetylase inhibitors deplete enhancer of zeste 2 and associated polycomb repressive complex 2 proteins in human acute leukemia cells

Categories:

Transfection

Authors:

Fiskus W, Pranpat M, Balasis M, Herger B, Rao R, Chinnaiyan A, Atadja P, Bhalla K

In:

Mol Cancer Ther (2006) 5(12): 3096-104

The collapsin response mediator protein 1 (CRMP-1) and the promyelocytic leukemia zinc finger protein (PLZF) bind to UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE), the key enzyme of sialic acid biosynthesis

Categories:

Transfection

Authors:

Weidemann W, Stelzl U, Lisewski U, Bork K, Wanker EE, Hinderlich S, Horstkorte R

In:

FEBS Lett (2006) 580(28-29): 6649-54

Phagocyte cell migration is mediated by phospholipases PLD1 and PLD2

Categories:

Transfection

Authors:

Lehman N, Di Fulvio M, McCray N, Campos I, Tabatabaian F, Gomez-Cambronero J

In:

Blood (2006) 108(10): 3564-72

Mechanisms of apoptosis sensitivity and resistance to the BH3 mimetic ABT-737 in acute myeloid leukemia

Categories:

Transfection

Authors:

Konopleva M, Contractor R, Tsao T, Samudio I, Ruvolo PP, Kitada S, Deng X, Zhai D, Shi YX, Sneed T, Verhaegen M, Soengas M, Ruvolo VR, McQueen T, Schober WD, Watt JC, Jiffar T, Ling X, Marini FC, Harris D, Dietrich M, Estrov Z, McCubrey J, May WS, Reed JC, Andreeff M

In:

Cancer Cell (2006) 10(5): 375-88

Integrin inhibition through Lyn-dependent cross talk from CXCR4 chemokine receptors in normal human CD34+ marrow cells

Categories:

Transfection

Authors:

Nakata Y, Tomkowicz B, Gewirtz AM, Ptasznik A

In:

Blood (2006) 107(11): 4234-9

The role of transcriptional coactivator TRAP220 in myelomonocytic differentiation

Categories:

Transfection

Authors:

Urahama N, Ito M, Sada A, Yakushijin K, Yamamoto K, Okamura A, Minagawa K, Hato A, Chihara K, Roeder RG and Matsui T

In:

Genes Cells (2005) 10(12): 1127-1137

Transcriptional Profiling Identifies Altered Intracellular Labile Iron Homeostasis as a Contributing Factor to the Toxicity of Adaphostin: Decreased Vascular Endothelial Growth Factor Secretion Is Independent of Hypoxia-Inducible Factor-1 Regulation

Categories:

Transfection

Authors:

Hose C, Kaur G, Sausville EA and Monks A

In:

Clin Cancer Res (2005) 11(17): 6370-6381

Arginine methylation provides epigenetic transcription memory for retinoid-induced differentiation in myeloid cells

Categories:

Transfection

Authors:

Balint BL, Szanto A, Madi A, Bauer UM, Gabor P, Benko S, Puskas LG, Davies PJ and Nagy L

In:

Mol Cell Biol (2005) 25(13): 5648-5663

RNAi of FACE1 protease results in growth inhibition of human cells expressing lamin A: implications for Hutchinson-Gilford progeria syndrome

Categories:

Transfection

Authors:

Gruber J, Lampe T, Osborn M and Weber K

In:

J Cell Sci (2005) 118(Pt 4): 689-696

Inositol hexakisphosphate kinase-2: A physiologic mediator of cell death

Categories:

Transfection

Authors:

Nagata E, Luo HR, Saiardi A, Bae BI, Suzuki N and Snyder SH

In:

J Biol Chem (2005) 280(2): 1634-1640

IRF-2 is involved in up-regulation of nonmuscle myosin heavy chain II-A gene expression during phorbol ester-induced promyelocytic HL-60 differentiation

Categories:

Transfection

Authors:

Chung MC and Kawamoto S

In:

J Biol Chem (2004) 279(53): 56042-56052

PTEN regulates motility but not directionality during leukocyte chemotaxis

Categories:

Transfection

Authors:

Lacalle RA, Gomez-Mouton C, Barber DF, Jimenez-Baranda S, Mira E, Martinez-A C, Carrera AC, Manes S

In:

J Cell Sci (2004) 117(Pt 25): 6207-6215

Short interfering RNA (siRNA) targeting the Lyn kinase induces apoptosis in primary, and drug-resistant, BCR-ABL1(+) leukemia cells

Categories:

Transfection

Authors:

Ptasznik A, Nakata Y, Kalota A, Emerson SG and Gewirtz AM

In:

Nat Med (2004) 10(11): 1187-1189

+ Show All