HeLa

cervical carcinoma (diagnosis later changed to adenocarcinoma) from 31 year old black woman, epitheloid

Cell Type:
Uterine/Vaginal Epith.
Tissue Origin:
cervix
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Basal Medium Eagle is a minimal medium suitable for a variety of cell types.  It is the historical precursor to Minimal Essential Medium (MEM) and was was formulated in 1955 for nutritional requirements of HeLa and mouse L cells.  BME can be used for culturing primary or diploid mammalian cells.

12-105 = BME with Earle's BSS, without L-glutamine 

Storage = 15ºC to 30ºC

Dulbecco's Modified Eagle Medium (DMEM) was developed in 1969 and is a modification of Basal Medium Eagle (BME) that differs from BME and MEM by the following characteristics:

  • Vitamins 4X greater than MEM. Vitamins and amino acids greater than BME
  • Types and quantities of amino acids greater than MEM and BME
  • Iron (ferric nitrate)

It is used in a wide range of mammalian cell culture applications.  The high glucose version is well suited to high density suspension culture. The low glucose formula is used for adherent dependent cells.

Storage = 2ºC to 8ºC

Liquid:
12-614 = 4.5 g/L glucose, without L-glutamine
12-604 = 4.5 g/L glucose, with L-glutamine
BE12-604/U1 = 4.5 g/L glucose, with Ultraglutamine I
12-914 = 4.5 g/L glucose, without L-glutamine (hybridoma screened)
12-917 = 4.5 g/L glucose, without L-glutamine or phenol red
12-709 = 4.5 g/L glucose and 25 mM HEPES, without L-glutamine
12-733= 4.5 g/L glucose, without L-glutamine or sodium pyruvate
12-741 = 4.5 g/L glucose, with L-glutamine, without sodium pyruvate

12-707 = 1.0 g/L glucose, without L-glutamine
12-708 = 1.0 g/L glucose and 25 mM HEPES, without L-glutamine

Powder:
15-614 = 4.5 g/L glucose, without L-glutamine or sodium pyruvate
15-604 = 4.5 g/L glucose, with L-glutamine or sodium pyruvate

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

PC-1™ is a low-protein, serum-free medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1 is formulated in a specially modified DMEM/F12 base and contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.
PC-1™ does not contain L-glutamine.

PC-1™ Liquid Base Medium is to be stored at 2°C-8°C.
The Supplement should be stored at -20°C.
When these two components are combined, the resulting PC-1™ Complete Medium is stable for 45 days at 2°C-8°C.

Once thawed, the appropriate volume of one vial of PC-1™ Supplement must be combined with the companion volume of PC-1™ Liquid Base Medium. Partial reconstitution or repeated freezing and thawing of the PC-1™ Supplement is not advised.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE CN-114 5e4 84.9% ±1 80.9% ±9 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE CN-114 3e5 83.6% ±1 83% ±12 Plasmid (general) 2 µg 100 µl 4D X-Unit
SE 96-CN-114 2e5 75% ±6 89% ±2 Plasmid (general) 0.4 µg 20 µl Shuttle
R I-013 5e5 20% ±2 85-90% Plasmid (general) 2 µg 100 µl I/II/2b
R I-013 5e5 70% ±3 85-90% Plasmid (general) 2 µg 100 µl I/II/2b
R A-028 1e6 13% ±7 95% Plasmid (general) 5 µg 100 µl I/II/2b
R A-028 1e6 57% ±7 95% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (41)

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Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
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In:
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Transfection 
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In:
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Transfection 
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In:
Nucleic Acids Res (2014) 42(21): 13440-51 
Categories:
Transfection 
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In:
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Categories:
Transfection 
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Categories:
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In:
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Categories:
Transfection 
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In:
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Categories:
Transfection 
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In:
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Categories:
Transfection 
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Categories:
Transfection 
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Categories:
Transfection 
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Categories:
Transfection 
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Categories:
Transfection 
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In:
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Categories:
Transfection 
Authors:
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In:
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Categories:
Transfection 
Authors:
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In:
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Categories:
Transfection 
Authors:
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J Immunol (2006) 177(9): 6471-6479 
Categories:
Transfection 
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Categories:
Transfection 
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Categories:
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Authors:
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Transfection 
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Categories:
Transfection 
Authors:
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Categories:
Transfection 
Authors:
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Categories:
Transfection 
Authors:
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EMBO J (2006) 25(1): 1-12 
Categories:
Transfection 
Authors:
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In:
Cell (2005) 123(7): 1293-305 
Categories:
Transfection 
Authors:
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In:
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Categories:
Transfection 
Authors:
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EMBO J (2005) 24(14): 2688-2699 
Categories:
Transfection 
Authors:
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Categories:
Transfection 
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Categories:
Transfection 
Authors:
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Categories:
Transfection 
Authors:
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J Biol Chem (2005) 280(17): 17371-17379 
Categories:
Transfection 
Authors:
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Categories:
Transfection 
Authors:
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Categories:
Transfection 
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Categories:
Transfection 
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Jager S, Bucci C, Tanida I, Ueno T, Kominami E, Saftig P and Eskelinen EL 
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J Cell Sci (2004) 117(Pt 20): 4837-4848 
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
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Categories:
Primary Cells and Media 
Authors:
Peng Zhou,#1 Xing-Lou Yang,#1 Xian-Guang Wang,#2 Ben Hu,1 Lei Zhang,1 Wei Zhang,1 Hao-Rui Si,1,3 Yan Zhu,1 Bei Li,1 Chao-Lin Huang,2 Hui-Dong Chen,2 Jing Chen,1,3 Yun Luo,1,3 Hua Guo,1,3 Ren-Di Jiang,1,3 Mei-Qin Liu,1,3 Ying Chen,1,3 Xu-Rui Shen,1,3 Xi Wang,1,3 Xiao-Shuang Zheng,1,3 Kai Zhao,1,3 Quan-Jiao Chen,1 Fei Deng,1 Lin-Lin Liu,4 Bing Yan,1 Fa-Xian Zhan,4 Yan-Yi Wang,1 Geng-Fu Xiao,1 and Zheng-Li Shi1 
In:
Nature () 579: 7798 
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