cells

Most common neuron type in the cerebellar cortex of the vertebrate brain

Cell Type:

Neurons (Brain)

Tissue Origin:

brain

Species:

rat

Research Area:

Neurobiology

Cell Characteristics:

Suspension

Recommended Media

Primary Neuron Growth Medium - Adult

The PNGM-A has been discontinued, Lonza is still offering the PNGM.

Primary Neuron Growth Medium - Adult is specifically developed for culturing adult primary rat neurons. This serum-free medium maintains pure populations of neuronal cells and allows for long-term maintenance and growth of cells in culture.
It is formulated from PNGM by the addition of CC-4511.

The complete PNGM-A kit contains:
- CC-3256 (PNBM basal Medium)
- CC-4462 (PNGM SingleQuots)
- CC-4511 (to formulate PNGM-A from PNGM)

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
Neuron, rat	O-003	7e6	38%	50-70%	Plasmid (general)	3 µg	100 µl	I/II/2b
Neuron, rat	G-013	7e6	38%	50-70%	Plasmid (general)	3 µg	100 µl	I/II/2b
Neuron, rat	G-013	5e6	50-60%	50-60%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations (20)

A FOXO-Pak1 transcriptional pathway controls neuronal polarity

Categories:

Transfection

Authors:

de la Torre-Ubieta L, Gaudillière B, Yang Y, Ikeuchi Y, Yamada T, DiBacco S, Stegmüller J, Schüller U, Salih DA, Rowitch D, Brunet A, Bonni A

In:

Genes Dev (2010) 24: 799–813

The role of Kalirin9 in p75/NgR-mediated RhoA activation in cerebellar granule neurons

Categories:

Transfection

Authors:

Harrington AW, Li QM, Tep C, Park JB, He Z, Yoon SO

In:

J Biol Chem (2008) epub: online

Mitochondrial Ca2+ overload underlies Abeta oligomers neurotoxicity providing an unexpected mechanism of neuroprotection by NSAIDs

Categories:

Transfection

Authors:

Sanz-Blasco S, Valero RA, Rodriguez-Crespo I, Villalobos C, Nunez L

In:

PLoS ONE (2008) 3(7): e2718

Bax siRNA promotes survival of cultured and allografted granule cell precursors through blockade of caspase-3 cleavage

Categories:

Transfection

Authors:

Zhokhov SS, Desfeux A, Aubert N, Falluel-Morel A, Fournier A, Laudenbach V, Vaudry H, Gonzalez BJ

In:

Cell Death Differ (2008) 15(6): 1042-53

Clathrin-dependent endocytosis is required for TrkB-dependent Akt-mediated neuronal protection and dendritic growth

Categories:

Transfection

Authors:

Zheng J, Shen WH, Lu TJ, Zhou Y, Chen Q, Wang Z, Xiang T, Zhu YC, Zhang C, Duan S, Xiong ZQ

In:

J Biol Chem (2008) 283(19): 13280-8

Synergistic neuroprotective effects of lithium and valproic acid or other histone deacetylase inhibitors in neurons: roles of glycogen synthase kinase-3 inhibition

Categories:

Transfection

Authors:

Leng Y, Liang MH, Ren M, Marinova Z, Leeds P, Chuang DM

In:

J Neurosci (2008) 28(10): 2576-88

Activation of double-stranded RNA-activated protein kinase by mild impairment of oxidative metabolism in neurons

Categories:

Transfection

Authors:

Wang X, Fan Z, Wang B, Luo J, Ke ZJ

In:

J Neurochem (2007) 103(6): 2380-90

Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway

Categories:

Transfection

Authors:

Hansen RK, Christensen C, Korshunova I, Kriebel M, Burkarth N, Kiselyov VV, Olsen M, Ostergaard S, Holm A, Volkmer H, Walmod PS, Berezin V, Bock E

In:

J Neurochem (2007) 103(4): 1396-407

Proteolytic cleavage of ataxin-7 by caspase-7 modulates cellular toxicity and transcriptional dysregulation

Categories:

Transfection

Authors:

Young JE, Gouw L, Propp S, Sopher BL, Taylor J, Lin A, Hermel E, Logvinova A, Chen SF, Chen S, Bredesen DE, Truant R, Ptacek LJ, La Spada AR, Ellerby LM

In:

J Biol Chem (2007) 282(41): 30150-60

Intracellular sortilin expression pattern regulates proNGF-induced naturally occurring cell death during development

Categories:

Transfection

Authors:

Nakamura K, Namekata K, Harada C, Harada T

In:

Cell Death Differ (2007) 14(8): 1552-4

TRPC channels promote cerebellar granule neuron survival

Categories:

Transfection

Authors:

Jia Y, Zhou J, Tai Y, Wang Y

In:

Nat Neurosci (2007) 10(5): 559-567

Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 upregulates cyclic AMP levels and promotes neurite outgrowth

Categories:

Transfection

Authors:

Tanaka S, Ishii K, Kasai K, Yoon SO, Saeki Y

In:

J Biol Chem (2007) 282(14): 10506-15

PACAP and C2-ceramide generate different AP-1 complexes through a MAP-kinase-dependent pathway: involvement of c-Fos in PACAP-induced Bcl-2 expression

Categories:

Transfection

Authors:

Aubert N, Falluel-Morel A, Vaudry D, Xifro X, Rodriguez-Alvarez J, Fisch C, de Jouffrey S, Lebigot JF, Fournier A, Vaudry H, Gonzalez BJ

In:

J Neurochem (2006) 99(4): 1237-50

Modulation of Gq-protein-coupled inositol trisphosphate and Ca2+ signaling by the membrane potential

Categories:

Transfection

Authors:

Billups D, Billups B, Challiss RA, Nahorski SR

In:

J Neurosci (2006) 26(39): 9983-95

Endogenous alpha-synuclein is induced by valproic acid through histone deacetylase inhibition and participates in neuroprotection against glutamate-induced excitotoxicity

Categories:

Transfection

Authors:

Leng Y, Chuang DM

In:

J Neurosci (2006) 26(28): 7502-12

MAG inhibits microtubule assembly by a Rho-kinase dependent mechanism

Categories:

Transfection

Authors:

Mimura F, Yamagishi S, Arimura N, Fujitani M, Kubo T, Kaibuchi K, Yamashita T

In:

J Biol Chem (2006) 281(23): 15970-9

Nucleofection of primary neurons

Categories:

Transfection

Authors:

Gartner A, Collin L, Lalli G

In:

Methods Enzymol (2006) 406: 374-88

Essential role of TRPC channels in the guidance of nerve growth cones by brain-derived neurotrophic factor

Categories:

Transfection

Authors:

Li Y, Jia YC, Cui K, Li N, Zheng ZY, Wang YZ and Yuan XB

In:

Nature (2005) 434(7035): 894-898

An NCAM-derived FGF-receptor agonist, the FGL-peptide, induces neurite outgrowth and neuronal survival in primary rat neurons

Categories:

Transfection

Authors:

Neiiendam JL, Kohler LB, Christensen C, Li S, Pedersen MV, Ditlevsen DK, Kornum MK, Kiselyov VV, Berezin V, Bock E

In:

J Neurochem (2004) 91(4): 920-935

Mechanism of NF-kappaB inactivation induced by survival signal withdrawal in cerebellar granule neurons

Categories:

Transfection

Authors:

Kovacs AD, Chakraborty-Sett S, Ramirez SH, Sniderhan LF, Williamson AL and Maggirwar SB

In:

Eur J Neurosci (2004) 20(2): 345-352

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Granule cell (CGC), rat

Recommended Media

Transfection Information

Citations (20)